Immunomodulatory effects of bone marrow mesenchymal stem cells overexpressing heme oxygenase-1: Protective effects on acute rejection following reduced-size liver transplantation in a rat model

2017 ◽  
Vol 313 ◽  
pp. 10-24 ◽  
Author(s):  
Zhong-Yang Shen ◽  
Bin Wu ◽  
Tao Liu ◽  
Yang Yang ◽  
Ming-Li Yin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Liver Transplantation ◽  
Mesenchymal Stem Cells ◽  
Acute Rejection ◽  
Rat Model ◽  
Heme Oxygenase 1 ◽  
Protective Effects ◽  
Immunomodulatory Effects ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Improvement of Liver Transplantation Outcome by Heme Oxygenase-1-Transduced Bone Marrow Mesenchymal Stem Cells in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Bin Wu ◽  
Hong-Li Song ◽  
Yang Yang ◽  
Ming-Li Yin ◽  
Bo-Ya Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Liver Transplantation ◽  
Mesenchymal Stem Cells ◽  
Heme Oxygenase ◽  
Transforming Growth Factor ◽  
The Other ◽  
Heme Oxygenase 1 ◽  
Immunomodulatory Effects ◽  
Marrow Mesenchymal Stem Cells

Bone marrow mesenchymal stem cells (BMMSCs) exert immunosuppressive activity in transplantation, and heme oxygenase-1 (HO-1) enhances their immunomodulatory effects. The aim of this study was to determine whether HO-1-transduced BMMSCs (HO-1/MSCs) improve rat liver transplantation (LTx) outcomes. Orthotopic LTx rejection models were treated with HO-1/MSCs, BMMSCs, HO-1, or normal saline, respectively. Our results showed a significant improvement in survival rates in the HO-1/BMMSCs group compared to the control groups. At all time points, liver function marker levels in the HO-1/MSCs group were significantly lower than in the other three groups; on POD 1, 7, and 14, the degree of rejection and apoptotic cells was significantly less in the HO-1/MSCs group than in the other three groups. Interleukin- (IL-) 10 and transforming growth factor-βlevels were significantly increased, while IL-2, IL-6, IL-17, IL-23, tumor necrosis factor-α, and interferon-γlevels were significantly decreased in the HO-1/MSCs group when compared to the other groups. Splenocyte Tregs were significantly increased by HO-1/MSCs compared with controls on POD 3, 5, 7, 10, 14, and 28. Summarily, we provide evidence that HO-1/MSCs improved allogeneic LTx outcomes by attenuating inflammatory responses and acute cellular rejection, as well as enhanced immunomodulatory effects compared with BMMSCs.

Bone marrow mesenchymal stem cells can improve the motor function of a Huntington's disease rat model

2011 ◽  
Vol 33 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Yufeng Jiang ◽  
Hailong Lv ◽  
Shanshan Huang ◽  
Huiping Tan ◽  
Yinong Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Motor Function ◽  
Rat Model ◽  
Marrow Mesenchymal Stem Cells

Transplantation of Bone Marrow Mesenchymal Stem Cells (BMSCs) Overexpressing Circular Zinc Finger Protein 609 Alleviates Diabetic Retinopathy

2021 ◽  
Vol 11 (12) ◽  
pp. 2497-2501
Author(s):  
Sheng Chen ◽  
Meiwen Tian ◽  
Shenwen Liu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Diabetic Retinopathy ◽  
Rat Model ◽  
Zinc Finger Protein ◽  
Retinal Thickness ◽  
Vitreous Body ◽  
Macular Volume ◽  
Marrow Mesenchymal Stem Cells

Diabetic retinopathy (DR) is a chronic complications and its pathogenesis remains unclear. This study aims to elucidate the underlying mechanism by how bone marrow mesenchymal stem cells (BMSCs) affects DR development in a rat model. A rat model of DR was established and injected with BMSCs overexpressing Cir-ZNF609 and shRNA Cir-ZNF609 to vitreous body followed by analysis of the retinal vascular permeability and macular retinal layers thickness, and the levels of HIF-1α, ICAM-1 and VEGF in rat retina by ELISA and immunohistochemistry. Injection of BMSCs overexpressing Cir-ZNF609 resulted in decreased HIF-1α ICAM-1 and VEGF expression, amelioration of retinal ganglion choriocapillaris injury and reducing ganglion cells. Twelve weeks after treatment, neovascularization took place and fibroblasts appeared with some nucleus disappearing and pigment taking off. Besides, permeability also elevated in the presence of overexpressing Cir-ZNF609 and penetration rate for Evans blue (16.36+3.25, 15.45±3.46 μg/g) was lower than healthy rats (28.66±2.08, 32.24±4.36 μg/g) and controls (26.93±3.03, 33.49±5.02 μg/g) (p < 0.01). Moreover, upregulation of Cir-ZNF609 decreased retinal thickness and macular volume in DR rats (p < 0.05). In conclusion, intravitreal injection of mouse BMSCs overexpressing Cir-ZNF609 alleviates retinal injury and decreases retinal thickness and macular volume, and enhances neovascularization. These evidence provides a novel insight into gene therapy for DR.

Bone Marrow Mesenchymal Stem Cells (BMSCs) Transplantation Ameliorates Joint Severity and Inflammation in Rats with Arthritis

2022 ◽  
Vol 12 (5) ◽  
pp. 1028-1033
Author(s):  
Liangbang Wu ◽  
Zhenhai Hou ◽  
Longbao Zheng ◽  
Zenghui Gu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Rat Model ◽  
Inflammatory Cells ◽  
Type Ii Collagen ◽  
Rankl Expression ◽  
Positive Expression ◽  
Marrow Mesenchymal Stem Cells ◽  
Bovine Type

This study analyzed the action of Bone marrow mesenchymal stem cells (BMSCs) transplantation on arthritis rat model. Arthritis rat model was established using bovine type II collagen and CFA. BMSCs phenotype was assessed by flow cytometry and pathological changes was analyzed by H&E staining along with analysis of joint severity by AI score, inflammation by ELISA as well as level of NPY, MMP-2, and MMP-9. The form of passaged BMSCs was spindle shaped with positive expression of CD29 and CD44. The structure of articular cavity in arthritis rats was disordered with infiltration of inflammatory cells which were ameliorated by BMSCs transplantation. In addition, BMSCs treatment also significantly reduced AI value, the level of VEGF, IL-17 and TNF-α as well as decreased RANK/RANKL expression and increased OPG level. In conclusion, BMSCs transplantation ameliorates inflammation and severity in arthritis rats possibly through regulation of RANK/OPG, indicating that it might be used for the treatment of arthritis patients.

Bone Marrow Mesenchymal Stem Cells (BMSCs) Restore Functional Endometrium in the Rat Model for Severe Asherman Syndrome

2018 ◽  
Vol 26 (3) ◽  
pp. 436-444 ◽  
Author(s):  
Lufen Gao ◽  
Zhongwei Huang ◽  
Haiyingjie Lin ◽  
Yuke Tian ◽  
Ping Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Rat Model ◽  
Marrow Mesenchymal Stem Cells
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