Bone Marrow Mesenchymal Stem Cells (BMSCs) Transplantation Ameliorates Joint Severity and Inflammation in Rats with Arthritis

This study analyzed the action of Bone marrow mesenchymal stem cells (BMSCs) transplantation on arthritis rat model. Arthritis rat model was established using bovine type II collagen and CFA. BMSCs phenotype was assessed by flow cytometry and pathological changes was analyzed by H&E staining along with analysis of joint severity by AI score, inflammation by ELISA as well as level of NPY, MMP-2, and MMP-9. The form of passaged BMSCs was spindle shaped with positive expression of CD29 and CD44. The structure of articular cavity in arthritis rats was disordered with infiltration of inflammatory cells which were ameliorated by BMSCs transplantation. In addition, BMSCs treatment also significantly reduced AI value, the level of VEGF, IL-17 and TNF-α as well as decreased RANK/RANKL expression and increased OPG level. In conclusion, BMSCs transplantation ameliorates inflammation and severity in arthritis rats possibly through regulation of RANK/OPG, indicating that it might be used for the treatment of arthritis patients.

Post operative echocardiographic follow up after mitral valve replacement by different types of biological valves

Objectives This retrospective study compares porcine with bovine bioprosthesis in mitral position with regard to valvular degeneration. Methods Between 2005 and 2019, 15 patients underwent mitral valve replacement with porcine Epic biological valve (St-Jude) and 15 patients underwent mitral valve replacement by bovine Carpentier –Edwards Perimount biological valve and follow up ecocardiogarphy was done to evaluate valvular degeneration of both types Results Bovine type showed longer durability than porcine and less valvular degeneration within 14 years of follow up regardless age of patient, cause of valve replacement, or lesion of valve Coclusion: Bovine bioprothesis are better than porcine regarding durability

Single-Stage Repair of Interrupted Aortic Arch With Aortopulmonary Window and Bovine-Type Aortic Arch Complicated by Left Subclavian Artery Origin Stenosis—A Case Report

An aortopulmonary window is known to be associated in 5% of interrupted aortic arch cases. The combination of these lesions with a bovine-type aortic arch is much more uncommon. We report its embryological explanation and successful single-stage surgical repair in a three-month-old infant.

Citrullinated fibrinogen is a target of auto-antibodies in interstitial lung disease in mice with collagen-induced arthritis

Interstitial lung disease (ILD) is a very common and lethal complication of rheumatoid arthritis (RA), yet its pathogenesis is not well understood, in part due to the lack of adequate animal models. Although collagen-induced arthritis (CIA) is the most widely used animal model for RA, the lung involvement occurring in this model has scarcely been studied. To evaluate the suitability of CIA as a model for RA-associated ILD (RA-ILD), we immunized DBA/1 mice with bovine type II collagen and characterized lung disease in this model. Histologic analyses revealed patchy interstitial infiltration of inflammatory cells in the peripheral regions of the lung, notably in the subpleural region, in mice with CIA. This pattern resembled usual interstitial pneumonia in humans, which is the most prevalent pattern in RA-ILD. Among infiltrates in the lung, CD11bhi macrophages of the M2 phenotype were most prominently increased. IgG and C3 were deposited in the subpleural region where inflammatory cells infiltrated. The sera from CIA mice contained auto-antibodies against citrullinated proteins, which are specific and predictive markers for RA. Protein citrullination was enhanced in the lung of CIA mice compared with naive mice, and citrullinated fibrinogen was primarily targeted by these auto-antibodies. The elevation of auto-antibodies against citrullinated proteins and their deposition in the lung with patchy subpleural preponderance suggest that CIA can serve as a model to study the pathogenesis of RA-ILD.

THE DYNAMICS OF EPIDEMIOLOGICAL SITUATION ON TUBERCULOSIS IN THE REPUBLIC OF DAGESTAN AND PERSPECTIVES OF ITS FURTHER AMELIORATION

During last ten years, in the Republic of Dagestan a steady decreasing of main epidemiological indices of tuberculosis (morbidity, mortality, etc.) is observed. The efficiency of treatment of patients with tuberculosis increased significantly. Besides, the new problems moved to forefront which don't fit with this trend. They are high contamination, presence of significant percentage of secondary forms of tuberculosis among children and adolescents, preservation of high morbidity of tuberculosis among individuals detected from contact with patients with BK+, annual increasing of of number of late recurrence and medication-resistant forms of tuberculosis of lungs, increasing of percentage of patients with detected mycobacterium of tuberculosis of bovine type - M.bovinus. Every one of these problems requires profound investigation for making address organizational decisions in groups of increased risk of development of tuberculosis.

Acellular Fish Skin Grafts and Pig Urinary Bladder Matrix Assessed in the Collagen-Induced Arthritis Mouse Model

It is vital that cellular- and tissue-based products (CTPs) used for wound treatment do not provoke autoimmunity. In this study, the immunogenic response to extracts of 2 CTPs of piscine and porcine origin was assessed in the collagen-induced arthritis model. Male DBA/1J mice were divided into 4 groups, each composed of 7 to 9 animals. Each animal was injected with one of following to assess their immune responses: (1) bovine type II collagen (100 µg) in Freund’s adjuvant, (2) extract of piscine skin (100 µg) in Freund’s adjuvant, (3) extract of porcine urinary bladder matrix (100 µg) in Freund’s adjuvant, or (4) Freund’s adjuvant alone (control) at the beginning of the experiment and 3 weeks later. Clinical signs of arthritis were assessed from week 5 onwards, and anti-type II and anti-type I collagen antibody immunoglobulin G (IgG) serum levels were measured before injections and 8 weeks after exposure using enzyme-linked immunosorbent assays. Only the mice exposed to bovine type II collagen developed clinical arthritis accompanied by very high anti-type II collagen IgG serum levels. Anti-type II collagen IgG serum levels were also detected in the porcine group but were undetectable in the piscine skin and control groups after 8 weeks. There were no significant differences in anti-type I collagen IgG serum levels among the groups. The results showed that piscine skin did not provoke systemic autoimmunity against type II collagens in DBA/1J mice.