Signaling pathways bridging fate determination of neural crest cells to glial lineages in the developing peripheral nervous system

2014 ◽  
Vol 26 (4) ◽  
pp. 673-682 ◽  
Author(s):  
Maulilio John Kipanyula ◽  
Wahabu Hamisi Kimaro ◽  
Faustin N. Yepnjio ◽  
Yousef H. Aldebasi ◽  
Mohammed Farahna ◽  
...  
Keyword(s):  
Nervous System ◽  
Neural Crest ◽  
Signaling Pathways ◽  
Peripheral Nervous System ◽  
Neural Crest Cells ◽  
Fate Determination

Fate determination of neural crest cells by NOTCH-mediated lateral inhibition and asymmetrical cell division during gangliogenesis

Development ◽  
2000 ◽  
Vol 127 (13) ◽  
pp. 2811-2821 ◽  
Author(s):  
Y. Wakamatsu ◽  
T.M. Maynard ◽  
J.A. Weston
Keyword(s):  
Cell Division ◽  
Neural Crest ◽  
Glial Cells ◽  
Dorsal Root Ganglia ◽  
Lateral Inhibition ◽  
Dorsal Root ◽  
Neural Crest Cells ◽  
Environmental Cues ◽  
Fate Determination

Avian trunk neural crest cells give rise to a variety of cell types including neurons and satellite glial cells in peripheral ganglia. It is widely assumed that crest cell fate is regulated by environmental cues from surrounding embryonic tissues. However, it is not clear how such environmental cues could cause both neurons and glial cells to differentiate from crest-derived precursors in the same ganglionic locations. To elucidate this issue, we have examined expression and function of components of the NOTCH signaling pathway in early crest cells and in avian dorsal root ganglia. We have found that Delta1, which encodes a NOTCH ligand, is expressed in early crest-derived neuronal cells, and that NOTCH1 activation in crest cells prevents neuronal differentiation and permits glial differentiation in vitro. We also found that NUMB, a NOTCH antagonist, is asymmetrically segregated when some undifferentiated crest-derived cells in nascent dorsal root ganglia undergo mitosis. We conclude that neuron-glia fate determination of crest cells is regulated, at least in part, by NOTCH-mediated lateral inhibition among crest-derived cells, and by asymmetric cell division.

scholarly journals Investigating Primary Cilia during Peripheral Nervous System Formation

2021 ◽  
Author(s):  
Elkhan Yusifov ◽  
Alexandre Dumoulin ◽  
Esther T. Stoeckli
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Neural Crest ◽  
Peripheral Nervous System ◽  
Primary Cilium ◽  
Developmental Disorders ◽  
Neural Crest Cells ◽  
Neuronal Cultures ◽  
System Formation

The primary cilium plays a pivotal role during embryonic development of vertebrates. It acts as a somatic signaling hub for specific pathways, such as sonic hedgehog signaling. In humans, mutations in genes that cause dysregulation of ciliogenesis or ciliary function lead to severe developmental disorders called ciliopathies. Beyond its obvious role in early morphogenesis, growing evidence points towards an essential function of the primary cilium in neural circuit formation in the central nervous system. However, very little is known about a potential role in the formation of the peripheral nervous system. Here, we investigated the presence of the primary cilium in neural crest cells and their derivatives in the trunk of the developing chicken embryo in vivo. We found that neural crest cells, sensory neurons, and boundary cap cells all bear a primary cilium during key stages of early peripheral nervous system formation. Moreover, we described differences in the ciliation of neuronal cultures of different populations from the peripheral and central nervous system. Our results offer a framework for further in vivo and in vitro investigations on specific roles that the primary cilium might play during peripheral nervous system formation.

scholarly journals Investigating Primary Cilia during Peripheral Nervous System Formation

2021 ◽  
Vol 22 (6) ◽  
pp. 3176
Author(s):  
Elkhan Yusifov ◽  
Alexandre Dumoulin ◽  
Esther T. Stoeckli
Keyword(s):  
Nervous System ◽  
Neural Crest ◽  
Peripheral Nervous System ◽  
Primary Cilium ◽  
Developmental Disorders ◽  
Neural Circuit ◽  
Neural Crest Cells ◽  
Neuronal Cultures ◽  
System Formation

The primary cilium plays a pivotal role during the embryonic development of vertebrates. It acts as a somatic signaling hub for specific pathways, such as Sonic Hedgehog signaling. In humans, mutations in genes that cause dysregulation of ciliogenesis or ciliary function lead to severe developmental disorders called ciliopathies. Beyond its role in early morphogenesis, growing evidence points towards an essential function of the primary cilium in neural circuit formation in the central nervous system. However, very little is known about a potential role in the formation of the peripheral nervous system. Here, we investigate the presence of the primary cilium in neural crest cells and their derivatives in the trunk of developing chicken embryos in vivo. We found that neural crest cells, sensory neurons, and boundary cap cells all bear a primary cilium during key stages of early peripheral nervous system formation. Moreover, we describe differences in the ciliation of neuronal cultures of different populations from the peripheral and central nervous systems. Our results offer a framework for further in vivo and in vitro investigations on specific roles that the primary cilium might play during peripheral nervous system formation.

A member of the RXR nuclear receptor family is expressed in neural-crest-derived cells of the developing chick peripheral nervous system

Development ◽  
1991 ◽  
Vol 111 (3) ◽  
pp. 771-778
Author(s):  
A. Rowe ◽  
N.S. Eager ◽  
P.M. Brickell
Keyword(s):  
Nervous System ◽  
Neural Crest ◽  
Peripheral Nervous System ◽  
Expression Patterns ◽  
Neural Crest Cells ◽  
Enteric Ganglia ◽  
Nerve Tracts ◽  
Alpha Beta ◽  
Posterior Trunk ◽  
Nuclear Receptor Family

Retinoic acid (RA) affects differentiation and morphogenesis in various developmental systems and is believed to act through nuclear RA receptors that belong to the steroid/thyroid hormone family of ligand-binding transcription factors. Three closely related receptors, RAR-alpha, -beta and -gamma, with distinct expression patterns, have been identified and a fourth receptor, hRXR-alpha, which responds to RA but which has low homology to RAR-alpha, -beta and -gamma, was recently discovered. Here we report the isolation of a cDNA clone encoding a chicken homologue of hRXR-alpha (cRXR) and show that a cRXR transcript of 2.5 kb is expressed in a range of embryonic chick tissues. By in situ hybridization to sections from stage 24 and stage 27 chick embryos, we show that cRXR transcripts are expressed at high levels in the liver and in elements of the developing peripheral nervous system derived from the neural crest, including dorsal root ganglia, cranial ganglia, enteric ganglia and peripheral nerve tracts. At stage 16, in the posterior trunk region, cRXR transcripts are expressed by cells in the neural crest and in neural crest cells migrating into the sclerotome, indicating that neural crest cells express cRXR transcripts before overt differentiation into peripheral nervous tissue. This distribution suggests a novel role for RA in the developing peripheral nervous system, mediated by cRXR. In addition, it identifies cRXR as a marker for a specific population of neural-crest-derived cells.

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