Studies on the restoration succession of PFU microbial communities in a pilot-scale microcosm

Ammonia is the main pollution factor of the aquatic environment in marine shrimp culture systems. In order to demonstrate the feasibility of the combination of biofloc technology and nitrifying biofilter for the ammonia removal, a 70-day production trial was conducted in a simplified pilot-scale hybrid biofloc-based recirculating aquaculture system (biofloc-RAS) with the intensive culture of Litopenaeus vannamei. Nitrogen dynamics and nitrifying microbial communities were investigated in three replicated systems simultaneously under the conditions of high feed loading and zero water exchange. Along with biofloc development in the culture tank and biofilm formation in the nitrifying biofilter during the trial, nitrification could be fastly and effectively established in the system, which was indicated by the dynamics of total ammonia nitrogen (TAN), NO2–-N, NO3–-N, and total nitrogen (TN) concentrations. Meanwhile, similar nitrifying microorganisms could be found between biofloc and biofilm, despite some differences in abundance, diversity, and composition of ammonia-oxidizing archaea and bacteria and nitrite-oxidizing bacteria. High TAN removal rate could be achieved and was significantly and positively correlated with abundances of these nitrifying microbial communities in both biofloc and biofilm, further indicating that both biofloc and biofilm could contribute highly to nitrification performance of the biofloc-RAS. The results of this study indicate a potential application of the biofloc-RAS in coastal intensive aquaculture.

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Comparison of microbial communities in pilot-scale bioreactors treating Bayer liquor organic wastes

Biodegradation ◽

10.1007/s10532-010-9412-6 ◽

2010 ◽

Vol 22 (2) ◽

pp. 397-407 ◽

Cited By ~ 7

Author(s):

Naomi J. McSweeney ◽

Jason J. Plumb ◽

Amanda L. Tilbury ◽

Hugh J. Nyeboer ◽

Matt E. Sumich ◽

...

Keyword(s):

Microbial Communities ◽

Pilot Scale ◽

Organic Wastes ◽

Bayer Liquor

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Secondary Effluent Treatment by Pilot-Scale Constructed Wetland and the Character of Its Microbial Communities

2009 3rd International Conference on Bioinformatics and Biomedical Engineering ◽

10.1109/icbbe.2009.5163365 ◽

2009 ◽

Author(s):

Jifeng Guo ◽

Yanjun Lu

Keyword(s):

Microbial Communities ◽

Constructed Wetland ◽

Pilot Scale ◽

Effluent Treatment ◽

Secondary Effluent

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Characterization of Microbial Communities Removing Nitrogen Oxides from Flue Gas: the BioDeNOx Process

Applied and Environmental Microbiology ◽

10.1128/aem.71.10.6345-6352.2005 ◽

2005 ◽

Vol 71 (10) ◽

pp. 6345-6352 ◽

Cited By ~ 30

Author(s):

Rajkumari Kumaraswamy ◽

Udo van Dongen ◽

J. Gijs Kuenen ◽

Wiebe Abma ◽

Mark C. M. van Loosdrecht ◽

...

Keyword(s):

Nitrogen Oxides ◽

16S Rrna ◽

Microbial Communities ◽

Flue Gas ◽

Iron Reduction ◽

Denaturing Gradient Gel Electrophoresis ◽

Pilot Scale ◽

Rrna Gene ◽

16S Rrna Analysis ◽

Gradient Gel Electrophoresis

ABSTRACT BioDeNOx is an integrated physicochemical and biological process for the removal of nitrogen oxides (NOx) from flue gases. In this process, the flue gas is purged through a scrubber containing a solution of Fe(II)EDTA2−, which binds the NOx to form an Fe(II)EDTA·NO2− complex. Subsequently, this complex is reduced in the bioreactor to dinitrogen by microbial denitrification. Fe(II)EDTA2−, which is oxidized to Fe(III)EDTA− by oxygen in the flue gas, is regenerated by microbial iron reduction. In this study, the microbial communities of both lab- and pilot-scale reactors were studied using culture-dependent and -independent approaches. A pure bacterial strain, KT-1, closely affiliated by 16S rRNA analysis to the gram-positive denitrifying bacterium Bacillus azotoformans, was obtained. DNA-DNA homology of the isolate with the type strain was 89%, indicating that strain KT-1 belongs to the species B. azotoformans. Strain KT-1 reduces Fe(II)EDTA·NO2− complex to N2 using ethanol, acetate, and Fe(II)EDTA2− as electron donors. It does not reduce Fe(III)EDTA−. Denaturing gradient gel electrophoresis analysis of PCR-amplified 16S rRNA gene fragments showed the presence of bacteria closely affiliated with members of the phylum Deferribacteres, an Fe(III)-reducing group of bacteria. Fluorescent in situ hybridization with oligonucleotide probes designed for strain KT-1 and members of the phylum Deferribacteres showed that the latter were more dominant in both reactors.

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Bioaugmentation of microbial communities in laboratory and pilot scale sequencing batch biofilm reactors using the TOL plasmid

Bioresource Technology ◽

10.1016/j.biortech.2008.09.048 ◽

2009 ◽

Vol 100 (5) ◽

pp. 1746-1753 ◽

Cited By ~ 48

Author(s):

S. Venkata Mohan ◽

Christina Falkentoft ◽

Y. Venkata Nancharaiah ◽

Belinda S. McSwain Sturm ◽

Pierre Wattiau ◽

...

Keyword(s):

Microbial Communities ◽

Pilot Scale ◽

Tol Plasmid ◽

Biofilm Reactors ◽

Sequencing Batch Biofilm Reactors

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Characterization of Microbial Communities in Pilot-Scale Constructed Wetlands with Salicornia for Treatment of Marine Aquaculture Effluents

Archaea ◽

10.1155/2018/7819840 ◽

2018 ◽

Vol 2018 ◽

pp. 1-12 ◽

Cited By ~ 6

Author(s):

Xiaona Ma ◽

Xingqiang Song ◽

Xian Li ◽

Songzhe Fu ◽

Meng Li ◽

...

Keyword(s):

Microbial Communities ◽

Constructed Wetlands ◽

High Throughput Sequencing ◽

Northern China ◽

Pilot Scale ◽

Ammonia Nitrogen ◽

Marine Aquaculture ◽

Salicornia Bigelovii ◽

Recirculating Aquaculture Systems ◽

Total Ammonia

Microorganisms play an essential role in the performance of constructed wetlands (CWs) for wastewater treatment. However, there has been limited discussion on the characteristics of microbial communities in CWs for treatment of effluents from marine recirculating aquaculture systems (RAS). This study is aimed at characterizing the microbial communities of pilot-scale CWs with Salicornia bigelovii for treatment of saline wastewater from a land-based Atlantic salmon RAS plant located in Northern China. Illumina high-throughput sequencing was employed to identify the profile of microbial communities of three CWs receiving wastewater under different total ammonia nitrogen (TAN) concentrations. Results of this study showed remarkable spatial variations in diversity and composition of microbial communities between roots and substrates in three CWs, with distinct response to different TAN concentrations. In particular, Proteobacteria, Firmicutes, Cyanobacteria, and Bacteroidetes were predominant in roots, while Cyanobacteria, Proteobacteria, Firmicutes, Verrucomicrobia, and Bacteroidetes were prevalent in substrates. Moreover, redundancy analysis indicated that specific functional genera, such as Nitrosopumilus, Vibrio, Pseudoalteromonas, Nitrospina, and Planctomyces, played key roles in the removal of nitrogen/phosphorus pollutants and growth of wetland plants. From a microorganism perspective, the findings of this study could contribute to better understanding of contaminants’ removal mechanism and improved management of CWs for treatment of effluents from land-based marine aquaculture.

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