scholarly journals Characterization of Microbial Communities in Pilot-Scale Constructed Wetlands with Salicornia for Treatment of Marine Aquaculture Effluents

Archaea ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Xiaona Ma ◽  
Xingqiang Song ◽  
Xian Li ◽  
Songzhe Fu ◽  
Meng Li ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Constructed Wetlands ◽  
High Throughput Sequencing ◽  
Northern China ◽  
Pilot Scale ◽  
Ammonia Nitrogen ◽  
Marine Aquaculture ◽  
Salicornia Bigelovii ◽  
Recirculating Aquaculture Systems ◽  
Total Ammonia

Microorganisms play an essential role in the performance of constructed wetlands (CWs) for wastewater treatment. However, there has been limited discussion on the characteristics of microbial communities in CWs for treatment of effluents from marine recirculating aquaculture systems (RAS). This study is aimed at characterizing the microbial communities of pilot-scale CWs with Salicornia bigelovii for treatment of saline wastewater from a land-based Atlantic salmon RAS plant located in Northern China. Illumina high-throughput sequencing was employed to identify the profile of microbial communities of three CWs receiving wastewater under different total ammonia nitrogen (TAN) concentrations. Results of this study showed remarkable spatial variations in diversity and composition of microbial communities between roots and substrates in three CWs, with distinct response to different TAN concentrations. In particular, Proteobacteria, Firmicutes, Cyanobacteria, and Bacteroidetes were predominant in roots, while Cyanobacteria, Proteobacteria, Firmicutes, Verrucomicrobia, and Bacteroidetes were prevalent in substrates. Moreover, redundancy analysis indicated that specific functional genera, such as Nitrosopumilus, Vibrio, Pseudoalteromonas, Nitrospina, and Planctomyces, played key roles in the removal of nitrogen/phosphorus pollutants and growth of wetland plants. From a microorganism perspective, the findings of this study could contribute to better understanding of contaminants’ removal mechanism and improved management of CWs for treatment of effluents from land-based marine aquaculture.

scholarly journals Characteristics of Ammonia Removal and Nitrifying Microbial Communities in a Hybrid Biofloc-RAS for Intensive Litopenaeus vannamei Culture: A Pilot-Scale Study

Water ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3000
Author(s):  
Wujie Xu ◽  
Yu Xu ◽  
Haochang Su ◽  
Xiaojuan Hu ◽  
Keng Yang ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Litopenaeus Vannamei ◽  
Removal Rate ◽  
Pilot Scale ◽  
Ammonia Nitrogen ◽  
Ammonia Removal ◽  
Shrimp Culture ◽  
Recirculating Aquaculture ◽  
Total Ammonia ◽  
High Feed

Ammonia is the main pollution factor of the aquatic environment in marine shrimp culture systems. In order to demonstrate the feasibility of the combination of biofloc technology and nitrifying biofilter for the ammonia removal, a 70-day production trial was conducted in a simplified pilot-scale hybrid biofloc-based recirculating aquaculture system (biofloc-RAS) with the intensive culture of Litopenaeus vannamei. Nitrogen dynamics and nitrifying microbial communities were investigated in three replicated systems simultaneously under the conditions of high feed loading and zero water exchange. Along with biofloc development in the culture tank and biofilm formation in the nitrifying biofilter during the trial, nitrification could be fastly and effectively established in the system, which was indicated by the dynamics of total ammonia nitrogen (TAN), NO2–-N, NO3–-N, and total nitrogen (TN) concentrations. Meanwhile, similar nitrifying microorganisms could be found between biofloc and biofilm, despite some differences in abundance, diversity, and composition of ammonia-oxidizing archaea and bacteria and nitrite-oxidizing bacteria. High TAN removal rate could be achieved and was significantly and positively correlated with abundances of these nitrifying microbial communities in both biofloc and biofilm, further indicating that both biofloc and biofilm could contribute highly to nitrification performance of the biofloc-RAS. The results of this study indicate a potential application of the biofloc-RAS in coastal intensive aquaculture.

Tilapia rearing with high rate algal pond effluent: ammonia surface loading rates and stocking densities effects

2018 ◽  
Vol 78 (1) ◽  
pp. 49-56
Author(s):  
I. A. Sánchez ◽  
R. K. X. Bastos ◽  
E. A. T. Lana
Keyword(s):  
Weight Gain ◽  
Oxygen Demand ◽  
Pilot Scale ◽  
Ammonia Nitrogen ◽  
High Rate ◽  
Surface Loading ◽  
Loading Rates ◽  
Total Weight Gain ◽  
Low Weight ◽  
Total Ammonia

Abstract In two pilot-scale experiments, fingerlings and juvenile of tilapia were reared in high rate algal pond (HRAP) effluent. The combination of three different total ammonia nitrogen (TAN) surface loading rates (SLR1 = 0.6, SLR2 = 1.2; SLR3 = 2.4 kg TAN·ha−1·d−1) and two fish stocking densities (D1 = 4 and D2 = 8 fish per tank) was evaluated during two 12-week experiments. Fingerlings total weight gain varied from 4.9 to 18.9 g, with the highest value (equivalent to 0.225 g·d−1) being recorded in SLR2-D1 treatment; however, high mortality (up to 67%) was recorded, probably due to sensitivity to ammonia and wide daily temperature variations. At lower water temperatures, juvenile tilapia showed no mortality, but very low weight gain. The fish rearing tanks worked as wastewater polishing units, adding the following approximate average removal figures on top of those achieved at the HRAP: 63% of total Kjeldahl nitrogen; 54% of ammonia nitrogen; 42% of total phosphorus; 37% of chemical oxygen demand; 1.1 log units of Escherichia coli.

scholarly journals Bacterial community shift and effects of the inclusion of a mixed psychrotrophic bacteria strain for sewage treatment in constructed wetland in winter seasons

2021 ◽  
Author(s):  
xiaoyan xu ◽  
Jie Jiang ◽  
Zhinan Guo ◽  
Lianglun Sun ◽  
Meizhen Tang
Keyword(s):  
Low Temperature ◽  
Constructed Wetlands ◽  
High Throughput Sequencing ◽  
Sewage Treatment ◽  
High Capacity ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Nitrogen And Phosphorus ◽  
Degradation Kinetic ◽  
Psychrotrophic Bacteria

Abstract The mechanism of wastewater treatment based on psychrophilic strains to improve the denitrification efficiency of constructed wetlands at low temperatures has already become a new hotspot. In this study, three mixed psychrophilic strains (Psychrobacter TM-1, Sphingobacterium TM-2 and Pseudomonas TM-3) with high capacity of denitrification were added into a vertical-flow constructed wetlands (CWs), and the effect of the mixed strains on CWs sewage treatment was evaluated. The removal efficiency of chemical oxygen demand (COD), ammonia nitrogen (NH4+-N), total nitrogen (TN) and total phosphorus (TP) was quantified to establish the degradation kinetic model and determine the best dosage of the mixed strains. The effect mechanism of the mixed strains on indigenous microbial community and the change of sewage treatment performance in low temperature constructed wetlands was clarified by high-throughput sequencing technology. The results showed that the mixed strains can effectively remove the organic pollutants (nitrogen and phosphorus) and the optimum dosage of the mixed strain was 2.5%,with average removal rates of 1.52, 2.12, 2.07 and 1.29 times than those of the control. Meanwhile, the dominant strains in the CWs were Proteobacteria (31.23–44.34%), Chloroflexi (12.04–19.05%), Actinobacteria (10.6-20.62%), Acidobacteria (8.23–11.65%), Firmicutes (2.23–15.95%) and Bacteroidetes (4.01–18.9%). These findings provide a basis for the removal of pollutants in constructed wetlands at low temperature.

scholarly journals Retrofitting Sea Cucumber Nursery Tanks to Recirculating Aquaculture Systems for Highly Intensive Litopenaeus vannamei Aquaculture

2021 ◽  
Vol 11 (20) ◽  
pp. 9478
Author(s):  
Yishuai Du ◽  
Jianping Xu ◽  
Li Zhou ◽  
Fudi Chen ◽  
Tianlong Qiu ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Sea Cucumber ◽  
Stocking Density ◽  
Ammonia Nitrogen ◽  
Average Weight ◽  
Recirculating Aquaculture ◽  
Nitrite Nitrogen ◽  
Innovative Methodology ◽  
Recirculating Aquaculture Systems ◽  
Total Ammonia

Designing good recirculating aquaculture systems (RASs) is challenging in shrimp aquaculture. In this study, two sets of RASs were constructed using sea cucumber nursery tanks for rearing Litopenaeus vannamei. Recirculating aquaculture was supported by key technologies such as sewage collection and aeration systems adapted to the rectangular tanks and technologies for the removal of sewage, shrimp shells, and dead individuals. Six-hundred and eighty-five thousand juveniles were selected for rearing in the newly constructed RASs, where the average stocking density was 1013 shrimp/m3. During the recirculating aquaculture period of 53 days, the water temperature of the tanks was 24–31 °C, the salinity was 25–32‰, the pH was 6.4–8.2, the DO was ≥ 4.9 mg/L, the concentration of total ammonia nitrogen (TAN) was maintained between 0.17 and 4.9 mg/L, the concentration of nitrite nitrogen (NO2-N) was between 0.12 and 4.7 mg/L, and the total number of Vibrio bacteria remained between 330 and 9700 cfu/mL. At the end of the experiment, the final average weight of individual shrimp was 13.43 g, and the average yield reached 12.92 kg/m3. The great improvement in growth performance marks a breakthrough in RAS technology of shrimp, and it supports the use of an innovative methodology for the retrofitting and utilization of idle sea cucumber nursery tanks.

scholarly journals Research about Organic Matter Removal and Biofilms Development of Pilot-Scale UV/H2O2-BAC Process

Water ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 565
Author(s):  
Wuchang Song ◽  
Congcong Li ◽  
Zhenqi Du ◽  
Jiangang Yue ◽  
Wenjun Sun ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Organic Matter ◽  
Organic Carbon ◽  
Microbial Communities ◽  
High Throughput Sequencing ◽  
Drinking Water Treatment ◽  
Pilot Scale ◽  
Process Efficiency ◽  
Organic Matter Removal ◽  
One Year

As a green advanced process for drinking water treatment, the UV/hydrogen peroxide (UV/H2O2) process has been gradually applied in China. To study the effect and mechanism of organic matter removal and the development of microbial communities in the UV/H2O2-biological activated carbon (UV/H2O2-BAC) process, a pilot-scale UV/H2O2-BAC system was built and operated over one year. Low water temperature affects the UV/H2O2 process efficiency, the biofilms in the BAC system were mature and stable after 240 days, and the contribution rate of BAC adsorption to dissolved organic carbon (DOC) removal was approximately 14.2% after one year of operation. The liquid chromatography-organic carbon detection (LC-OCD) analysis shows that UV/H2O2 process can increase the amounts of Low Molecular Weight (LMW) neutrals, and the specific UV absorbance (SUVA254) value is not suitable for predicting Trihalomethanes (THMs) precursor contents in water after UV/H2O2 treatment. High-throughput sequencing results prove that microbial species in the middle section are the most abundant compared to those in the influent and effluent sections, hydrogen peroxide has lower inhibition on the development of microbial community than ozone and the low concentration of hydrogen peroxide (<0.25 mg/L) promotes the development of the microbial communities, hydrogen peroxide can reduce Proteobacteria abundance by inhibiting the growth of anaerobes. Acidobacteria may have a certain contribution to the degradation of soil organic matter (SOM), and the effluent section of BAC with low DOC concentration cannot form the dominant species of Rhodobacter.

scholarly journals Upgrading northern municipal lagoons: Total nitrogen removal and phosphorus assimilation at ultra-low temperatures

2021 ◽  
Author(s):  
Patrick M. D'Aoust ◽  
Simon Vincent ◽  
Guillaume Leblond ◽  
Raheleh Arabgol ◽  
Richard Hérard ◽  
...  
Keyword(s):  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Low Temperatures ◽  
Pilot Scale ◽  
Ammonia Nitrogen ◽  
Biofilm Reactor ◽  
Sulfide Toxicity ◽  
Total Ammonia ◽  
Treatment Train ◽  
Total Nitrogen Removal

In this study, a municipal lagoon with high wintertime effluent total ammonia nitrogen (TAN) concentrations was upgraded with a pilot-scale NIT-NIT-DENIT moving bed biofilm reactor (MBBR) treatment train to characterize its effluent over wintertime operation, investigate the feasibility of upgrading lagoons to achieve substantial biological total nitrogen removal across ultra-low temperatures (0.6 – 3.0°C) and investigate nitrification inhibition pathways in facultative lagoon systems at ultra-low temperatures. Throughout the study, it was observed that the system substantially reduced total nitrogen (TN) and total phosphorus (TP) effluent concentrations by an average of 69.0 ± 24.5% and 74.7 ± 20.1%, respectively, with average TN and TP concentrations exiting the treatment train of 7.60 ± 5.60 mg-N/L and 0.05 ± 0.02 mg-P/L, respectively, indicating the feasibility of upgrading municipal lagoons to meet increasing stringent effluent standards to ensure the perenniality of water resources. Furthermore, it was observed that sulfide toxicity may play an important role in the inhibition of nitrifying organisms in lagoons.

scholarly journals Application of hybrid electrocoagulation–filtration methods in the pretreatment of marine aquaculture wastewater

2021 ◽  
Vol 83 (6) ◽  
pp. 1315-1326
Author(s):  
Jianping Xu ◽  
Yishuai Du ◽  
Tianlong Qiu ◽  
Li Zhou ◽  
Ye Li ◽  
...  
Keyword(s):  
Pore Size ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Marine Aquaculture ◽  
Recirculating Aquaculture ◽  
System A ◽  
Filtration System ◽  
Total Ammonia ◽  
Combination Modes ◽  
Aquaculture Wastewater

Abstract The aim of this study was to provide technical means and data support for enhancing the filtration pretreatment capacity of a recirculating aquaculture system. A continuous flow electrocoagulation (EC)–filtration system was designed and its application in the pretreatment of marine aquaculture wastewater was studied. The influences of anode combination modes, hydraulic retention times (HRTs) of the EC reactor and filter pore sizes on the water treatment capacity were investigated. Results showed that EC could significantly enhance the treatment efficiency of the filtration equipment used in subsequent steps. Al-Fe electrodes used as anode led to better processing capacity of this system, and the optimum anode was 3Al + Fe. With the increase of HRT and decrease of filter pore size, the enhanced effect of the EC process on the filter was more obvious. When the current density was 19.22 A/m2, the anode was 3Al + Fe, the HRT was 4.5 min and the filter pore size was 45 μm, the removal efficiency of the system for Vibrio, chemical oxygen demand, total ammonia nitrogen, nitrite nitrogen (NO2−-N), nitrate nitrogen (NO3−-N) and total nitrogen was 69.55 ± 0.93%, 48.99 ± 1.42%, 57.06 ± 1.28%, 34.09 ± 2.27%, 18.47 ± 1.88% and 55.26 ± 1.42%, respectively, and the energy consumption was (26.25 ± 4.95) × 10−3kWh/m3.

scholarly journals Ammonia Recovery from Digestate Using Gas-Permeable Membranes: A Pilot-Scale Study

Environments ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 133
Author(s):  
Berta Riaño ◽  
Beatriz Molinuevo-Salces ◽  
Matías B. Vanotti ◽  
María Cruz García-González
Keyword(s):  
Pilot Scale ◽  
Ammonia Nitrogen ◽  
Land Application ◽  
Frequent Change ◽  
Permeable Membrane ◽  
A Value ◽  
Trapping Solution ◽  
Ammonia Recovery ◽  
Total Ammonia ◽  
Eu Project

The reduction and recovery of nitrogen (N) from anaerobically digested manure (digestate) is desirable to mitigate N-related emissions, mainly ammonia and nitrate, derived from digestate land application in nutrient-saturated zones. This work reports the results of a gas-permeable membrane (GPM) pilot-scale plant to recover ammonia from digestate in the framework of the EU project Ammonia Trapping. The total ammonia nitrogen (TAN) concentration in digestate was reduced by 34.2% on average (range 9.4–57.4%). The recovery of TAN in the trapping solution in the form of a (NH4)2SO4 solution averaged 55.3% of the removed TAN, with a TAN recovery rate of 16.2 g N m−2 d−1 (range between 14.5 and 21.0 g N m−2 d−1). The TAN concentration in the trapping solution achieved a value of up to 35,000 mg N L−1. The frequent change of the trapping solution has been proven as an efficient strategy to improve the overall performance of the GPM technology.

Sign in / Sign up

Export Citation Format

Share Document