Effect of endophyte-infection on growth parameters and Cd-induced phytotoxicity of Cd-hyperaccumulator Solanum nigrum L.

Solanum nigrum L. is a medicinal plant of solanaceae family with distinguished therapeutic properties. Traditionally, S. nigrum. had been used as an anti-tumorgenic, antioxidant, hepatoprotective, diuretic, and antipyretic agent. The most important alkaloid member in this plant is solanine. Therefore, this study was conducted to utilize tissue culture techniques for the enhancement of solanine production in the in vitro grown cultures of this promising neglected plant. For callus growth and development experimental part, the highest callus growth parameters (callus diameter (21.4 mm) and callus fresh weight (2202.4 mg) were obtained in callus grown on Murashige & Skoog MS media supplemented with 2,4-Dichlorophenoxyacetic acid (2.0 mg·L-1) plus 1.5 mg·L-1 Thidiazuron. Similar trend was also obtained in cell suspension culture experiment, as maximum growth was recorded at similar hormone combination. Moreover, High-performance liquid chromatography analysis revealed that, solanine was affected by growth regulator type and concentration. The highest solanine levels were obtained when the explants were treated with 6-benzylaminopurine at level of 2.0 mg·L-1, as solanine content reached up to (2.61, 1.53 mg.g-1) for callus and cell suspension, respectively, while, microshoot contained the highest solanine (4.52 mg.g-1 DW) at 6-benzylaminopurine level of 1.6 mg.L-1. Additionally, carbon source had positively affected solanine level, where 0.2 M sucrose resulted in production of the highest amounts (3.13, 2.03 and 1.20 mg.g-1 DW) of solanine in microshoots, callus and cell suspension, respectively. Also, exposing microshoots and callus to light intensity of (100 µmol.m -2 s-1) yielded the highest solanine content (4.03 and 1.26 mg.g-1 DW, respectively),while the lowest solanine levels (1.50 and 0.48 mg.g-1 DW) were observed in plant material exposed to the lowest light intensity treatment (25 µmol.m -2 s-1) .

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EFFECTS OF THE REUSE OF UNTREATED WATER FROM THE AVO’O RIVER (CAMEROON) CONTAMINATED BY FECAL SLUDGE FOR THE GROWTH OF SOLANUM NIGRUM L.

Innovare Journal of Agricultural Sciences ◽

10.22159/ijags.2021.v9i3.40833 ◽

2021 ◽

pp. 4-7

Author(s):

TSAMA VN ◽

KOM FM ◽

DJUYOM WV ◽

KONE N ◽

FONKOU T

Keyword(s):

Membrane Filtration ◽

Growth Parameters ◽

Control Site ◽

Solanum Nigrum ◽

Polluted Water ◽

High Concentration ◽

Filtration Method ◽

Discharge Area ◽

Solanum Nigrum L ◽

Faecal Sludge

Objective: The main objective of this research was to evaluate the growth parameters and sanitary quality of Solanum nigrum L. irrigated with polluted water from Avo’o river in Nomayos. Methods: Faecal sludge, water from Avo’o River and Solanum nigrum L., were analysed with respect to microbiological and parasitological parameters. Four points (S0: Control site, located in Mbankomo at 3 km away from faecal sludge discharge area; S1: located at 810 m before the faecal sludge discharge area; S2: located at 100 m away from the faecal sludge discharge area; and S3: located at 350 m after the faecal sludge discharge area) were considered for sampling events. For faecal indicator (FC: Faecal coliforms and FS: Faecal Streptococci), the membrane filtration method was used. Helminthes eggs were determined using the method of Bailenger, modified by Rodier. The growth parameters of S. nigrum L., measured in different site mentioned above, were plant height, number, length and width of fresh leaves. Results: High concentration of FC (6857 ± 5180) and FS (2500 ± 1876 CFU/100 ml), as well as helminths eggs (273 ± 186 eggs/L) were obtained in Avo’o water samples. The strong correlations (r=0.9) clearly show that the waters of the Avo’o stream influence the growth of S. nigrum L. Conclusion: Despite the best growth of Solanum Nigrum L. irrigated with water from Avo’o River, high concentration of parasites was obtained on their leaves (43 eggs/100 g) highlight the necessity of a better management of faecal sludge in this city.

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Mechanical Factors of Infl uence on Biological Effi ciency of Solanum Nigrum L.

Agricultural science and practice ◽

10.15407/agrisp1.02.020 ◽

2014 ◽

Vol 1 (2) ◽

pp. 20-23

Author(s):

O. Ivashchenko ◽

O. Ivashchenko

Keyword(s):

Solanum Nigrum ◽

Black Nightshade ◽

Effi Ciency ◽

Weed Plants ◽

Sensitivity Level ◽

Solanum Nigrum L ◽

Small Plot ◽

Mechanical Factors ◽

The Moment ◽

Working Out

Aim. Studying of black nightshade young plants’ response to the induced mechanical stresses. Methods. Researches conducted in small plot fi eld experiments. Results. Change of sensitivity level of Solanum nigrum L. plants depending on phases of their development at the moment of damage of elevated parts has been proven. Owing to loss of the surface capable to photosynthesis, there is an essential decrease in volumes of photosynthesis at plants of weed survived and their possibilities of ontogeny passage. The deep induced dis- stresses reduce biological effi ciency of plants, their ability to accumulate weight and to form seeds and even lead them to death. Conclusions. The defi ned principles of response of weed plants to the induced mechanical dis-stresses are can be used for working out and ecological receptions of crops protection from weeds.

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