EFFECTS OF THE REUSE OF UNTREATED WATER FROM THE AVO’O RIVER (CAMEROON) CONTAMINATED BY FECAL SLUDGE FOR THE GROWTH OF SOLANUM NIGRUM L.

Objective: The main objective of this research was to evaluate the growth parameters and sanitary quality of Solanum nigrum L. irrigated with polluted water from Avo’o river in Nomayos. Methods: Faecal sludge, water from Avo’o River and Solanum nigrum L., were analysed with respect to microbiological and parasitological parameters. Four points (S0: Control site, located in Mbankomo at 3 km away from faecal sludge discharge area; S1: located at 810 m before the faecal sludge discharge area; S2: located at 100 m away from the faecal sludge discharge area; and S3: located at 350 m after the faecal sludge discharge area) were considered for sampling events. For faecal indicator (FC: Faecal coliforms and FS: Faecal Streptococci), the membrane filtration method was used. Helminthes eggs were determined using the method of Bailenger, modified by Rodier. The growth parameters of S. nigrum L., measured in different site mentioned above, were plant height, number, length and width of fresh leaves. Results: High concentration of FC (6857 ± 5180) and FS (2500 ± 1876 CFU/100 ml), as well as helminths eggs (273 ± 186 eggs/L) were obtained in Avo’o water samples. The strong correlations (r=0.9) clearly show that the waters of the Avo’o stream influence the growth of S. nigrum L. Conclusion: Despite the best growth of Solanum Nigrum L. irrigated with water from Avo’o River, high concentration of parasites was obtained on their leaves (43 eggs/100 g) highlight the necessity of a better management of faecal sludge in this city.

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Manipulating Some Culturing Conditions Enhances Production of Solanine in Microshoots, Callus and Cell suspension Cultures of Solanum nigrum L.: A Wild Medicinal Plant

Journal of Agricultural and Marine Sciences [JAMS] ◽

10.24200/jams.vol24iss0pp51-62 ◽

2020 ◽

Vol 24 ◽

pp. 51

Author(s):

Rida A Shibli

Keyword(s):

Light Intensity ◽

Medicinal Plant ◽

Cell Suspension ◽

Growth Parameters ◽

Cell Suspension Cultures ◽

Solanum Nigrum ◽

Callus Growth ◽

Culture Techniques ◽

Solanum Nigrum L

Solanum nigrum L. is a medicinal plant of solanaceae family with distinguished therapeutic properties. Traditionally, S. nigrum. had been used as an anti-tumorgenic, antioxidant, hepatoprotective, diuretic, and antipyretic agent. The most important alkaloid member in this plant is solanine. Therefore, this study was conducted to utilize tissue culture techniques for the enhancement of solanine production in the in vitro grown cultures of this promising neglected plant. For callus growth and development experimental part, the highest callus growth parameters (callus diameter (21.4 mm) and callus fresh weight (2202.4 mg) were obtained in callus grown on Murashige & Skoog MS media supplemented with 2,4-Dichlorophenoxyacetic acid (2.0 mg·L-1) plus 1.5 mg·L-1 Thidiazuron. Similar trend was also obtained in cell suspension culture experiment, as maximum growth was recorded at similar hormone combination. Moreover, High-performance liquid chromatography analysis revealed that, solanine was affected by growth regulator type and concentration. The highest solanine levels were obtained when the explants were treated with 6-benzylaminopurine at level of 2.0 mg·L-1, as solanine content reached up to (2.61, 1.53 mg.g-1) for callus and cell suspension, respectively, while, microshoot contained the highest solanine (4.52 mg.g-1 DW) at 6-benzylaminopurine level of 1.6 mg.L-1. Additionally, carbon source had positively affected solanine level, where 0.2 M sucrose resulted in production of the highest amounts (3.13, 2.03 and 1.20 mg.g-1 DW) of solanine in microshoots, callus and cell suspension, respectively. Also, exposing microshoots and callus to light intensity of (100 µmol.m -2 s-1) yielded the highest solanine content (4.03 and 1.26 mg.g-1 DW, respectively),while the lowest solanine levels (1.50 and 0.48 mg.g-1 DW) were observed in plant material exposed to the lowest light intensity treatment (25 µmol.m -2 s-1) .

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Effect of endophyte-infection on growth parameters and Cd-induced phytotoxicity of Cd-hyperaccumulator Solanum nigrum L.

Chemosphere ◽

10.1016/j.chemosphere.2012.07.005 ◽

2012 ◽

Vol 89 (6) ◽

pp. 743-750 ◽

Cited By ~ 70

Author(s):

Yong Wan ◽

Shenglian Luo ◽

Jueliang Chen ◽

Xiao Xiao ◽

Liang Chen ◽

...

Keyword(s):

Growth Parameters ◽

Solanum Nigrum ◽

Endophyte Infection ◽

Solanum Nigrum L

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Microbiological Estimation of Nasal Spray Containing Naltrexone Hydrochloride

Drug development & registration ◽

10.33380/2305-2066-2020-9-4-116-120 ◽

2020 ◽

Vol 9 (4) ◽

pp. 116-120

Author(s):

Yu. M. Domnina ◽

V. V. Suslov ◽

N. E. Grammatikova ◽

S. A. Kedik

Keyword(s):

Nasal Spray ◽

Membrane Filtration ◽

Antimicrobial Effect ◽

The State ◽

High Concentration ◽

Optimal Test ◽

Filtration Method ◽

Naltrexone Hydrochloride ◽

Test Conditions ◽

Qualitative Nature

Introduction. In order to standardize the quality indicators during the expected shelf life of the developed drug naltrexone hydrochloride in the form of a nasal spray containing a high concentration of poloxamer and benzalkonium chloride as a preservative, a microbiological study was carried out. The possibility of using the membrane filtration method for testing prototypes recommended by the State Pharmacopoeia XIV.Aim. Study and selection of test conditions for the «microbiological purity» indicator of nasal spray samples containing naltrexone hydrochloride.Materials and methods. As an object of research, a naltrexone hydrochloride nasal spray was used. When analyzing the microbiological purity, the membrane filtration method recommended in the State Pharmacopoeia XIV.Results and discussion. As part of the study, it was found that the samples of the drug meet the requirements for a microbiological indicator for drugs of category 2. Testing the suitability of the method for samples of the dosage form showed that the antimicrobial effect of the drug was completely removed by washing the filter, which was proved by inoculation of indicator test microorganisms, the quantitative and qualitative nature of growth, which did not differ from the control without the drug.Conclusion. As a result of the studies carried out, the optimal test conditions for the «Microbiological purity» indicator for the nasal spray containing naltrexone hydrochloride were selected and substantiated.

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Mechanical Factors of Infl uence on Biological Effi ciency of Solanum Nigrum L.

Agricultural science and practice ◽

10.15407/agrisp1.02.020 ◽

2014 ◽

Vol 1 (2) ◽

pp. 20-23

Author(s):

O. Ivashchenko ◽

O. Ivashchenko

Keyword(s):

Solanum Nigrum ◽

Black Nightshade ◽

Effi Ciency ◽

Weed Plants ◽

Sensitivity Level ◽

Solanum Nigrum L ◽

Small Plot ◽

Mechanical Factors ◽

The Moment ◽

Working Out

Aim. Studying of black nightshade young plants’ response to the induced mechanical stresses. Methods. Researches conducted in small plot fi eld experiments. Results. Change of sensitivity level of Solanum nigrum L. plants depending on phases of their development at the moment of damage of elevated parts has been proven. Owing to loss of the surface capable to photosynthesis, there is an essential decrease in volumes of photosynthesis at plants of weed survived and their possibilities of ontogeny passage. The deep induced dis- stresses reduce biological effi ciency of plants, their ability to accumulate weight and to form seeds and even lead them to death. Conclusions. The defi ned principles of response of weed plants to the induced mechanical dis-stresses are can be used for working out and ecological receptions of crops protection from weeds.

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An Improved Membrane Filtration Method for Enumeration of Faecal Coliforms and E. Coli by a Fluorogenic β-Glucuronidase Assay

Water Science & Technology ◽

10.2166/wst.1993.0357 ◽

1993 ◽

Vol 27 (3-4) ◽

pp. 267-270 ◽

Cited By ~ 4

Author(s):

M. T. Augoustinos ◽

N. A. Grabow ◽

B. Genthe ◽

R. Kfir

Keyword(s):

Escherichia Coli ◽

Water Samples ◽

Membrane Filtration ◽

Faecal Coliforms ◽

Environmental Waters ◽

Filtration Method ◽

E Coli ◽

Wide Range ◽

Pure Cultures ◽

Glucuronidase Assay

A fluorogenic β-glucuronidase assay comprising membrane filtration followed by selective enumeration on m-FC agar at 44.5°C and further confirmation using tlie 4-metliylumbelliferyl-β-D-glucuronide (MUG) containing medium was evaluated for the detection of Escherichia coli in water. A total of 200 typical blue and non-typical blue colonies were isolated from sea and fresh water samples using initial selective enumeration on m-FC agar. Pure cultures of the selected colonies were further tested using the MUG assay and identified using the API 20E method. Of the colonies tested which were shown to be positive using the MUG assay 99.4% were Escherichia coli. The results of this study indicate the combination of the m-FC method followed by the MUG assay to be highly efficient for the selection and confirmation of E. coli from a wide range of environmental waters.

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