Effects of chlorpyrifos on in vitro sex steroid production and thyroid follicular development in adult and larval Lake Sturgeon, Acipenser fulvescens

Chemosphere ◽  
2015 ◽  
Vol 132 ◽  
pp. 179-187 ◽  
Author(s):  
Catherine Brandt ◽  
Duncan C. Burnett ◽  
Liane Arcinas ◽  
Vince Palace ◽  
W. Gary Anderson
Keyword(s):  
Follicular Development ◽  
Lake Sturgeon ◽  
Sex Steroid ◽  
Acipenser Fulvescens ◽  
Steroid Production

Follicular steroidogenesis in vitro in cyclic and PMSG/hCG-treated gilts

1991 ◽  
Vol 71 (4) ◽  
pp. 1257-1260
Author(s):  
T. Wiesak ◽  
R. T. Hardin ◽  
G. R. Foxcroft ◽  
M. G. Hunter
Keyword(s):  
Key Words ◽  
Follicular Development ◽  
Steroid Production ◽  
Functional Differences ◽  
Key Words Pig

Basal steroid production in vitro by comparable follicles from cyclic and PMSG/hCG-treated gilts was similar. Follicular responses to LH in vitro varied, however, confirming earlier evidence for functional differences in the pattern of follicular development in immature gilts in response to exogenous gonadotropins compared with naturally cyclic gilts. Key words: Pig, gonadotrophin, follicle, steroidogenesis, steroids

scholarly journals Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro

2021 ◽  
Vol 10 (15) ◽  
pp. e53101522488
Author(s):  
Rebeca Magalhães Pedrosa Rocha ◽  
Marcello Rubessa ◽  
Laritza Ferreira de Lima ◽  
Ana Flávia Bezerra da Silva ◽  
Rebecca Winters ◽  
...  
Keyword(s):  
Formation Rate ◽  
Follicular Development ◽  
Hormone Production ◽  
Steroid Production ◽  
Preantral Follicles ◽  
North Carolina State University ◽  
Base Medium ◽  
The Media ◽  
Survival And Growth

To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (α-MEM) as a base medium, and to evaluate the effects of Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating Hormone (FSH) on the in vitro development and steroid production of isolated porcine preantral follicles. Porcine secondary follicles were cultured in NCSU23 or α-MEM media for 4 days. Once α-MEM was determined to be the optimal culture medium, secondary follicles were cultured in α-MEM alone or supplemented with FSH (1.5 ng/mL), AMH (50 ng/mL) or the combination of the two hormones. Follicle development was evaluated by measuring follicular growth, morphology and hormone production. There was no difference between the media NCSU23 and α-MEM in terms of follicle survival and growth (P > 0.05). However, at day 2, the antrum formation rate tended to be (P < 0.074) higher in α-MEM than NCSU23. At day 4 of culture, the estradiol and progesterone secretion were higher in α-MEM than NCSU23 (P < 0.01), while the opposite was observed for testosterone (P < 0.01). The addition of AMH and/or FSH did not affect follicular survival and growth. Nevertheless, the secretion of estradiol and progesterone induced by FSH was reduced with AMH (P < 0.01). α-MEM is a more effective base medium than NCSU23 for the in vitro follicular development of pig preantral follicles and AMH reduces the steroid production induced by FSH.

Retinoic acid hydroxylation and teratogenesis in lake sturgeon (Acipenser fulvescens) from the St. Lawrence River and Abitibi region, Quebec

1999 ◽  
Vol 56 (8) ◽  
pp. 1428-1436 ◽  
Author(s):  
Christian Doyon ◽  
Réjean Fortin ◽  
Philip A Spear
Keyword(s):  
Retinoic Acid ◽  
Active Form ◽  
Liver Microsomes ◽  
Gonadal Development ◽  
Lake Sturgeon ◽  
Acipenser Fulvescens ◽  
Liver Sample ◽  
Cytochrome P 450 ◽  
St Lawrence River

All-trans-retinoic acid (RA) is an extremely active form of vitamin A; however, excessive or insufficient concentrations elicit anomalies including those associated with limb and craniofacial development. Lake sturgeon (Acipenser fulvescens) from the St. Lawrence River and tributaries were estimated to have a 2.9% incidence of fin and craniofacial malformations. Among larvae raised in artificial streams, a greater incidence of fin malformations (6.3%; p < 0.001) occurred in St. Lawrence River sturgeon compared with larvae from the Abitibi region. To test the hypothesis that the differences in rate of malformations may be associated with metabolic imbalances of RA, cytochrome P-450 dependent conversion to 4-hydroxyretinoic acid (4-OH-RA) was investigated. Optimal assay conditions are described for in vitro 4-OH-RA production in liver microsomes. Enzyme activity tended to decrease with increasing age and gonadal development in fish collected from Abitibi, but no statistically significant effects of sex, age, length, or gonadal development were discerned. The rate of 4-OH-RA formation was 3.5-fold greater (p < 0.0001) in the St. Lawrence sturgeon while the concentrations of cytochrome P-450 inducing PCBs (expressed as dioxin toxic equivalents) were 20-fold greater in a composite liver sample of the St. Lawrence sturgeon. Liver retinoid concentrations were negatively correlated (p < 0.0001) with RA hydroxylation rate. These results are consistent with the possibility that cytochrome P-450 dependent RA metabolism increased by chemical contaminants is associated with developmental anomalies in the St. Lawrence sturgeon.

scholarly journals Response in Growth, Scute Development, and Whole-Body Ion Composition of Acipenser fulvescens Reared in Water of Differing Chemistries

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1419
Author(s):  
Janet Genz ◽  
Rachael Hicks
Keyword(s):  
River Water ◽  
Growth And Development ◽  
Lake Sturgeon ◽  
Whole Body ◽  
Acipenser Fulvescens ◽  
Water Type ◽  
Ion Composition ◽  
Ion Concentrations ◽  
Before And After ◽  
Yolk Absorption

In fishes, environmental ion availability can have substantial effects on growth and development. This study examined the development of Lake Sturgeon in response to the varying environmental ion availability that they experience as part of a conservation stocking program. We reared sturgeon in natural water from the Coosa River, which had higher concentrations of Mg2+, Na+, and Zn2+ than standard hatchery conditions, while [Ca2+] at the Warm Springs National Fish Hatchery was 2× higher than in the Coosa River. Eggs were hatched in each water type and the larvae were sampled at time points before and after yolk absorption during the first 8 weeks of development. Total length and weight in WSNFH larvae were significantly higher than larvae in Coosa River water starting at 8 dph, indicating that growth was dependent on the different environmental ion levels. Concentrations of the ions of interest were also determined for whole-body acid digests of the exposed Lake Sturgeon. We found that Lake Sturgeon reared in Coosa River water had significantly higher magnesium and zinc than Lake Sturgeon reared in WSNFH water (p < 0.05), while calcium was significantly higher in WSNFH than Coosa River water. This difference shows that different environmental ion concentrations also impact the overall development of larval Lake Sturgeon.

scholarly journals Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using Mice

2021 ◽  
Vol 10 (13) ◽  
pp. 2757
Author(s):  
Xia Hao ◽  
Amandine Anastácio ◽  
Kenny A. Rodriguez-Wallberg
Keyword(s):  
Oocyte Maturation ◽  
In Vitro Maturation ◽  
Follicular Development ◽  
Clinical Situation ◽  
Experimental Proof ◽  
Secondary Follicle ◽  
17Β Estradiol ◽  
Follicle Size ◽  
And Control

Fertility preservation through ovarian stimulation, aiming at cryopreserving mature oocytes or embryos, is sometimes unsuccessful. This clinical situation deserves novel approaches to overcome infertility following cancer treatment in patients facing highly gonadotoxic treatment. In this controlled experimental study, we investigated the feasibility of in-vitro culturing secondary follicles isolated from superovulated ovaries of mice recently treated with gonadotropins. The follicle yields of superovulated ovaries were 45.9% less than in unstimulated controls. Follicles from superovulated ovaries showed faster growth pace during the initial 7 days of culture and secreted more 17β-estradiol by the end of culture vs controls. Parameters reflecting the outcome of follicular development and oocyte maturation competence in vitro were similar between superovulated and control groups, with a similar follicle size at the end of culture and around 70% survival. Nearly half of cultured follicles met the criteria for in-vitro maturation in both groups and approximately 60% of those achieved a mature MII oocyte, similarly in both groups. Over 60% of obtained MII oocytes displayed normal-looking spindle and chromosome configurations, without significant differences between the groups. Using a validated follicle culture system, we demonstrated the feasibility of secondary follicle isolation, in-vitro culture and oocyte maturation with normal spindle and chromosome configurations obtained from superovulated mice ovaries.

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