Follicular steroidogenesis in vitro in cyclic and PMSG/hCG-treated gilts

1991 ◽  
Vol 71 (4) ◽  
pp. 1257-1260
Author(s):  
T. Wiesak ◽  
R. T. Hardin ◽  
G. R. Foxcroft ◽  
M. G. Hunter
Keyword(s):  
Key Words ◽  
Follicular Development ◽  
Steroid Production ◽  
Functional Differences ◽  
Key Words Pig

Basal steroid production in vitro by comparable follicles from cyclic and PMSG/hCG-treated gilts was similar. Follicular responses to LH in vitro varied, however, confirming earlier evidence for functional differences in the pattern of follicular development in immature gilts in response to exogenous gonadotropins compared with naturally cyclic gilts. Key words: Pig, gonadotrophin, follicle, steroidogenesis, steroids

Effects of chlorpyrifos on in vitro sex steroid production and thyroid follicular development in adult and larval Lake Sturgeon, Acipenser fulvescens

Chemosphere ◽  
2015 ◽  
Vol 132 ◽  
pp. 179-187 ◽  
Author(s):  
Catherine Brandt ◽  
Duncan C. Burnett ◽  
Liane Arcinas ◽  
Vince Palace ◽  
W. Gary Anderson
Keyword(s):  
Follicular Development ◽  
Lake Sturgeon ◽  
Sex Steroid ◽  
Acipenser Fulvescens ◽  
Steroid Production

scholarly journals Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro

2021 ◽  
Vol 10 (15) ◽  
pp. e53101522488
Author(s):  
Rebeca Magalhães Pedrosa Rocha ◽  
Marcello Rubessa ◽  
Laritza Ferreira de Lima ◽  
Ana Flávia Bezerra da Silva ◽  
Rebecca Winters ◽  
...  
Keyword(s):  
Formation Rate ◽  
Follicular Development ◽  
Hormone Production ◽  
Steroid Production ◽  
Preantral Follicles ◽  
North Carolina State University ◽  
Base Medium ◽  
The Media ◽  
Survival And Growth

To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (α-MEM) as a base medium, and to evaluate the effects of Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating Hormone (FSH) on the in vitro development and steroid production of isolated porcine preantral follicles. Porcine secondary follicles were cultured in NCSU23 or α-MEM media for 4 days. Once α-MEM was determined to be the optimal culture medium, secondary follicles were cultured in α-MEM alone or supplemented with FSH (1.5 ng/mL), AMH (50 ng/mL) or the combination of the two hormones. Follicle development was evaluated by measuring follicular growth, morphology and hormone production. There was no difference between the media NCSU23 and α-MEM in terms of follicle survival and growth (P > 0.05). However, at day 2, the antrum formation rate tended to be (P < 0.074) higher in α-MEM than NCSU23. At day 4 of culture, the estradiol and progesterone secretion were higher in α-MEM than NCSU23 (P < 0.01), while the opposite was observed for testosterone (P < 0.01). The addition of AMH and/or FSH did not affect follicular survival and growth. Nevertheless, the secretion of estradiol and progesterone induced by FSH was reduced with AMH (P < 0.01). α-MEM is a more effective base medium than NCSU23 for the in vitro follicular development of pig preantral follicles and AMH reduces the steroid production induced by FSH.

scholarly journals Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using Mice

2021 ◽  
Vol 10 (13) ◽  
pp. 2757
Author(s):  
Xia Hao ◽  
Amandine Anastácio ◽  
Kenny A. Rodriguez-Wallberg
Keyword(s):  
Oocyte Maturation ◽  
In Vitro Maturation ◽  
Follicular Development ◽  
Clinical Situation ◽  
Experimental Proof ◽  
Secondary Follicle ◽  
17Β Estradiol ◽  
Follicle Size ◽  
And Control

Fertility preservation through ovarian stimulation, aiming at cryopreserving mature oocytes or embryos, is sometimes unsuccessful. This clinical situation deserves novel approaches to overcome infertility following cancer treatment in patients facing highly gonadotoxic treatment. In this controlled experimental study, we investigated the feasibility of in-vitro culturing secondary follicles isolated from superovulated ovaries of mice recently treated with gonadotropins. The follicle yields of superovulated ovaries were 45.9% less than in unstimulated controls. Follicles from superovulated ovaries showed faster growth pace during the initial 7 days of culture and secreted more 17β-estradiol by the end of culture vs controls. Parameters reflecting the outcome of follicular development and oocyte maturation competence in vitro were similar between superovulated and control groups, with a similar follicle size at the end of culture and around 70% survival. Nearly half of cultured follicles met the criteria for in-vitro maturation in both groups and approximately 60% of those achieved a mature MII oocyte, similarly in both groups. Over 60% of obtained MII oocytes displayed normal-looking spindle and chromosome configurations, without significant differences between the groups. Using a validated follicle culture system, we demonstrated the feasibility of secondary follicle isolation, in-vitro culture and oocyte maturation with normal spindle and chromosome configurations obtained from superovulated mice ovaries.

scholarly journals GLUCOSE CONSUMPTION AND LACTIC ACID PRODUCTION BY ERYTHROCYTES FROM DOUBLE-MUSCLED CATTLE

1982 ◽  
Vol 62 (3) ◽  
pp. 751-757 ◽  
Author(s):  
J. A. BASARAB ◽  
R. T. BERG ◽  
J. R. THOMPSON
Keyword(s):  
Lactic Acid ◽  
Key Words ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Age Groups ◽  
Phenotypic Expression ◽  
Glucose Consumption ◽  
Molar Ratio ◽  
Breed Group

The in vitro glucose consumption and lactic acid production by erythrocytes from 20 cattle of a Beef Synthetic (SY) breed group and 25 cattle of a double-muscled (DM) breed group were determined. There were three age groups and two sexes within each breed group. Animals within the DM breed group were categorized as either phenotypically normal- to moderate-muscled (DM carriers) or extreme-muscled (extreme DM) based on the phenotypic expression of the double-muscling trait and on their breeding history. Both DM phenotypes had higher (P < 0.01) erythrocyte glucose consumption and lactic acid production than normal-muscled, noncarrier cattle of the SY breed group. Extreme DM cattle were not different (P > 0.05) in either their erythrocyte glucose consumption or lactic acid production compared with DM carriers. No difference (P > 0.05) due to breed or phenotype was observed in the molar ratio of lactic acid produced to glucose consumed by erythrocytes. These results suggest that carriers of the Double Muscled Syndrome, regardless of phenotypic expression of the double-muscling trait, have a higher rate of erythrocyte glycolysis than normal cattle. Key words: Cattle, double muscled, erythrocyte, glucose, lactic acid

scholarly journals Effects of IGF-I bioavailability on bovine preantral follicular development in vitro

Reproduction ◽  
2007 ◽  
Vol 133 (6) ◽  
pp. 1121-1128 ◽  
Author(s):  
Fiona H Thomas ◽  
Bruce K Campbell ◽  
David G Armstrong ◽  
Evelyn E Telfer
Keyword(s):  
Follicular Development ◽  
Follicle Development ◽  
Dependent Manner ◽  
Igf Binding Proteins ◽  
Preantral Follicles ◽  
Follicle Diameter ◽  
Igf I ◽  
Igf Binding ◽  
Development In Vitro

The aim of this study was to determine the effect of regulation of IGF-I bioavailability on preantral follicle development in vitro. Bovine preantral follicles were cultured for 6 days in serum-free medium with increasing doses of Long R3 (LR3) IGF-I (an analog with low affinity for IGF-binding proteins (IGFBPs)), or human recombinant IGF-I (hrIGF-I). Follicle diameter and estradiol production were measured every second day. On day 6, ratios of oocyte/follicle diameter and oocyte morphology were assessed by histological examination, and IGFBP-2 and -3 were detected by immunocytochemistry and in situ hybridization respectively. Both types of IGF-I increased follicle diameter in a dose-dependent manner (P < 0.05) and increased estradiol production over control levels (P < 0.05). However, follicles treated with LR3 IGF-I and the highest concentration of hrIGF-I (1000 ng/ml) had smaller oocyte/follicle ratios, and increased oocyte degeneration, compared with controls or follicles treated with physiological concentrations of hrIGF-I (P < 0.05). IGFBPs were detected in cultured preantral follicles, indicating a requirement for regulation of IGF bioavailability during the early stages of follicular development. Specifically, IGFBP-3 mRNA was found to be expressed in oocytes, and IGFBP-2 immunoreactivity was detected in oocytes and granulosa cells of cultured follicles. In summary, the regulation of IGF-I bioavailability by IGFBPs is necessary for the co-ordination of oocyte and follicle development in vitro.

scholarly journals AKT is involved in granulosa cell autophagy regulation via mTOR signaling during rat follicular development and atresia

Reproduction ◽  
2014 ◽  
Vol 147 (1) ◽  
pp. 73-80 ◽  
Author(s):  
JongYeob Choi ◽  
MinWha Jo ◽  
EunYoung Lee ◽  
DooSeok Choi
Keyword(s):  
Cell Death ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Apoptotic Cell ◽  
Follicular Development ◽  
Negative Regulator ◽  
Metabolic Clearance ◽  
Akt Inhibitor ◽  
Western Blots

In this study, we examined whether granulosa cell autophagy during follicular development and atresia was regulated by the class I phosphoinositide-3 kinase/protein kinase B (AKT) pathway, which is known to control the activity of mammalian target of rapamycin (mTOR), a major negative regulator of autophagy. Ovaries and granulosa cells were obtained using an established gonadotropin-primed immature rat model that induces follicular development and atresia. Autophagy was evaluated by measuring the expression level of microtubule-associated protein light chain 3-II (LC3-II) using western blots and immunohistochemistry. The activity of AKT and mTOR was also examined by observing the phosphorylation of AKT and ribosomal protein S6 kinase (S6K) respectively. After gonadotropin injection, LC3-II expression was suppressed and phosphorylation of AKT and S6K increased in rat granulosa cells. By contrast, gonadotropin withdrawal by metabolic clearance promoted LC3-II expression and decreased phosphorylation of AKT and S6K. In addition,in-vitroFSH treatment of rat granulosa cells also indicated inhibition of LC3-II expression accompanied by a marked increase in phosphorylation of AKT and S6K. Inhibition of AKT phosphorylation using AKT inhibitor VIII suppressed FSH-mediated phosphorylation of S6K, followed by an increase in LC3-II expression. Furthermore, co-treatment with FSH and AKT inhibitor increased the levels of apoptosis and cell death of granulosa cells compared with the single treatment with FSH. Taken together, our findings indicated that AKT-mediated activation of mTOR suppresses granulosa cell autophagy during follicular development and is involved in the regulation of apoptotic cell death.

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