Off-line comprehensive two-dimensional high-performance liquid chromatography system with size exclusion column and reverse phase column for separation of complex traditional Chinese medicine Qingkailing injection

2006 ◽  
Vol 1127 (1-2) ◽  
pp. 207-213 ◽  
Author(s):  
Sai Ma ◽  
Lingxin Chen ◽  
Guoan Luo ◽  
Kangning Ren ◽  
Juanfang Wu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chinese Medicine ◽  
High Performance ◽  
Size Exclusion ◽  
Two Dimensional ◽  
Reverse Phase ◽  
Qingkailing Injection ◽  
Liquid Chromatography System ◽  
Phase Column

scholarly journals Separation of New Coumarin Glycosides from Toddalia asiatica Using Offline Two-Dimensional High-Performance Liquid Chromatography

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 428 ◽  
Author(s):  
Yan Li ◽  
Shi-Wei Sun ◽  
Xiao-Yi Zhang ◽  
Yang Liu ◽  
Xiao-Hong Liu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Purity ◽  
High Performance ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Two Dimensional ◽  
Toddalia Asiatica ◽  
Phase Column ◽  
Reversed Phase Column

Coumarins and flavonoids are the major constituents of Toddalia asiatica. The separation and purification of ingredients from T. asiatica is an important procedure to acquire high-purity compounds for subsequent pharmacological investigation to discover leading compounds. In the present work, an offline two-dimensional high-performance liquid chromatography (HPLC) method was successfully established for the separation of high-purity glycosides from T. asiatica. Based on the separation results obtained with two different chromatographic stationary phases, a phenyl-bonded silica-based reversed-phase column was employed as the first HPLC preparation, and three fractions were obtained from the sample. Then, the fractions were isolated and purified on an octadecyl-bonded silica-based reversed-phase column to obtain high-purity compounds in the second HPLC separation. As a result, three coumarin glycosides, including two undescribed and one known, along with one known flavonoid glycoside with more than 98% purity were isolated from the sample. The structures of the isolated compounds were elucidated on the basis of extensive spectroscopic evidence derived from optical rotation, mass spectrometry, and nuclear magnetic resonance experiments. Two-dimensional HPLC with different stationary phases has the potential to be an efficient method for the separation of high-purity compounds from T. asiatica.

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