Analysis of Camellia sinensis green and black teas via ultra high performance liquid chromatography assisted by liquid–liquid partition and two-dimensional liquid chromatography (size exclusion×reversed phase)

2012 ◽  
Vol 1222 ◽  
pp. 29-37 ◽  
Author(s):  
Camila T. Scoparo ◽  
Lauro M. de Souza ◽  
Nessana Dartora ◽  
Guilherme L. Sassaki ◽  
Philip A.J. Gorin ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Camellia Sinensis ◽  
High Performance ◽  
Reversed Phase ◽  
Size Exclusion ◽  
Two Dimensional

scholarly journals Separation of New Coumarin Glycosides from Toddalia asiatica Using Offline Two-Dimensional High-Performance Liquid Chromatography

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 428 ◽  
Author(s):  
Yan Li ◽  
Shi-Wei Sun ◽  
Xiao-Yi Zhang ◽  
Yang Liu ◽  
Xiao-Hong Liu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Purity ◽  
High Performance ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Two Dimensional ◽  
Toddalia Asiatica ◽  
Phase Column ◽  
Reversed Phase Column

Coumarins and flavonoids are the major constituents of Toddalia asiatica. The separation and purification of ingredients from T. asiatica is an important procedure to acquire high-purity compounds for subsequent pharmacological investigation to discover leading compounds. In the present work, an offline two-dimensional high-performance liquid chromatography (HPLC) method was successfully established for the separation of high-purity glycosides from T. asiatica. Based on the separation results obtained with two different chromatographic stationary phases, a phenyl-bonded silica-based reversed-phase column was employed as the first HPLC preparation, and three fractions were obtained from the sample. Then, the fractions were isolated and purified on an octadecyl-bonded silica-based reversed-phase column to obtain high-purity compounds in the second HPLC separation. As a result, three coumarin glycosides, including two undescribed and one known, along with one known flavonoid glycoside with more than 98% purity were isolated from the sample. The structures of the isolated compounds were elucidated on the basis of extensive spectroscopic evidence derived from optical rotation, mass spectrometry, and nuclear magnetic resonance experiments. Two-dimensional HPLC with different stationary phases has the potential to be an efficient method for the separation of high-purity compounds from T. asiatica.

Size exclusion and reversed-phase high-performance liquid chromatography/UV for routine control of thermal processing of cows' and donkey milk major proteins

2012 ◽  
Vol 79 (2) ◽  
pp. 224-231 ◽  
Author(s):  
Carina Pinho ◽  
Zita E. Martins ◽  
Catarina Petisca ◽  
Agata M. Figurska ◽  
Olívia Pinho ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thermal Processing ◽  
High Performance ◽  
Reversed Phase ◽  
Size Exclusion ◽  
Donkey Milk ◽  
Rp Hplc ◽  
External Standard Method ◽  
Thermally Processed

Cows' and donkey milks (raw and thermally processed) and respective whey were analysed for quantification of major proteins. Two different chromatographic approaches, size exclusion (SE-HPLC) and reversed-phase high performance liquid chromatography (RP-HPLC) both coupled to UV detection were used. Usefulness of these methods for routine control of the effect of thermal processing was evaluated. The external standard method was used to calibrate the SE-HPLC and RP-HPLC systems. Concerning quantification of β-lactoglobulin (β-lg), α-lactalbumin (α-la), lysozyme (lys), and total casein (cn), no significant differences between results obtained by SE-HPLC and by RP-HPLC (t-test, P>0·05) were observed for raw milks and whey. Heating of cows' milk promoted aggregation of denatured proteins as observed by SE-HPLC, whereas α-la and β-lg from donkey milk were stable to thermal processing at 100°C (5 min). Lys was quantified in donkey raw milk and whey however, in thermally processed donkey milk lys was denatured and could not be quantified by HPLC.

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