Iptycene-based stationary phase with three-dimensional aromatic structure for highly selective separation of H-bonding analytes and aromatic isomers

2016 ◽  
Vol 1445 ◽  
pp. 135-139 ◽  
Author(s):  
Xiaohong Yang ◽  
Ying Han ◽  
Meiling Qi ◽  
Chuanfeng Chen

We have made a detailed theoretical and experimental study of the three-dimensional diffraction pattern decorating the geometrical-optics caustic surface whose form is the elliptic umbilic catastrophe in Thom’s classification. This caustic has three sheets joined along three parabolic cusped edges (‘ribs’) which touch at one singular point (the ‘focus’). Experimentally, the diffraction catastrophe was studied in light refracted by a water droplet 'lens’ with triangular perimeter, and photographed in sections perpendicular to the symmetry axis of the pattern. Theoretically, the pattern was represented by a diffraction integral E(x,y,z) , which was studied numerically through computer simulations and analytically by the method of stationary phase. Particular attention was concentrated on the ‘dislocation lines’ where | E | vanishes, since these can be considered as a skeleton on which the whole diffraction pattern is built. Within the region bounded by the caustic surface the interference of four rays produces hexagonal diffraction maxima stacked in space like the atoms of a distorted crystal with space group R3m. The dislocation lines not too close to the ribs form hexagonally puckered rings. On receding from the focus and approaching the ribs, these rings approach one another and eventually join to form ‘hairpins’, each arm of which is a tightly wound sheared helix that develops asymptotically into one of the dislocations of the cusp diffraction catastrophe previously studied by Pearcey. Outside the caustic there are also helical dislocation lines, this time formed by interference involving a complex ray. There is close agreement, down to the finest details, between observation, exact computation of E(x,y,z) , and the four-wave stationary-phase approximation.


Open Biology ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 180066 ◽  
Author(s):  
Gisela Klauck ◽  
Diego O. Serra ◽  
Alexandra Possling ◽  
Regine Hengge

Bacterial biofilms are large aggregates of cells embedded in an extracellular matrix of self-produced polymers. In macrocolony biofilms of Escherichia coli , this matrix is generated in the upper biofilm layer only and shows a surprisingly complex supracellular architecture. Stratified matrix production follows the vertical nutrient gradient and requires the stationary phase σ S (RpoS) subunit of RNA polymerase and the second messenger c-di-GMP. By visualizing global gene expression patterns with a newly designed fingerprint set of Gfp reporter fusions, our study reveals the spatial order of differential sigma factor activities, stringent control of ribosomal gene expression and c-di-GMP signalling in vertically cryosectioned macrocolony biofilms. Long-range physiological stratification shows a duplication of the growth-to-stationary phase pattern that integrates nutrient and oxygen gradients. In addition, distinct short-range heterogeneity occurs within specific biofilm strata and correlates with visually different zones of the refined matrix architecture. These results introduce a new conceptual framework for the control of biofilm formation and demonstrate that the intriguing extracellular matrix architecture, which determines the emergent physiological and biomechanical properties of biofilms, results from the spatial interplay of global gene regulation and microenvironmental conditions. Overall, mature bacterial macrocolony biofilms thus resemble the highly organized tissues of multicellular organisms.


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