On-line separation and quantification of virus antigens of different serotypes in multivalent vaccines by capillary zone electrophoresis: A case study for quality control of foot-and-mouth disease virus vaccines

2021 ◽  
Vol 1637 ◽  
pp. 461834
Author(s):  
Yanmin Song ◽  
Yanli Yang ◽  
Xuan Lin ◽  
Qizu Zhao ◽  
Zhengjun Li ◽  
...  
Keyword(s):  
Quality Control ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease Virus ◽  
Zone Electrophoresis ◽  
On Line ◽  
Capillary Zone ◽  
Line Separation ◽  
Virus Antigens

Highly Purified Foot-and-Mouth Disease Virus: Optical and Biological Measurements during Zone Electrophoresis in a Glucose Density Gradient

1966 ◽  
Vol 21 (8) ◽  
pp. 774-782 ◽  
Author(s):  
H. D. Matheka ◽  
H. L. Bachrach ◽  
R. Trautman
Keyword(s):  
Density Gradient ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Optical Methods ◽  
High Passage ◽  
Free Zone ◽  
Mouth Disease Virus ◽  
Zone Electrophoresis ◽  
Foot And Mouth

Highly purified, concentrated foot-and-mouth disease virus, type A, was homogeneous and monodisperse by carrier-free zone electrophoresis in a glucose density gradient. Virus mobilities in veronal-acetate buffer, pH 8.6 and ionic strength 0.1, were identical whether determined by infectivity or optical methods. Low passage calf-kidney virus had a significantly higher mobility, 3.28 × 10-5 cm2/volt · sec, than high passage virus, 2.84 × 10-5 cm2/volt · sec. Single passages of the high passage virus in baby hamster kidney cells did not change its mobility. Regardless of passage history, storage at 4°C in 0.05 M phosphate at pH 7.5 increased mobilities to about 3.75 × 10-5 cm2/volt · sec with retention of infectivity; some virions broke down to protein and ribonucleic acid. These electrophoretically distinct viruses possessed different absorbance-temperature profiles but identical sedimentation constants.

Freeze-drying foot-and-mouth disease virus antigens. II. For use in the ELISA

1990 ◽  
Vol 30 (2) ◽  
pp. 183-195 ◽  
Author(s):  
N.P. Ferris ◽  
R.M. Philpot ◽  
J.M. Oxtoby ◽  
R.M. Armstrong
Keyword(s):  
Disease Virus ◽  
Freeze Drying ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Virus Antigens

HSV-1 amplicon vectors that direct the in situ production of foot-and-mouth disease virus antigens in mammalian cells can be used for genetic immunization

Vaccine ◽  
2010 ◽  
Vol 28 (46) ◽  
pp. 7363-7372 ◽  
Author(s):  
Alejandra D’Antuono ◽  
Andrea S. Laimbacher ◽  
Jose La Torre ◽  
Virginia Tribulatti ◽  
Carina Romanutti ◽  
...  
Keyword(s):  
Disease Virus ◽  
Mammalian Cells ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Genetic Immunization ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Virus Antigens ◽  
In Situ Production
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