Single-tube test for autoantibodies to glutamic acid decarboxylase and proinsulin as first-line screening for autoimmunity in adult-onset diabetic patients

2004 ◽  
Vol 113 (3) ◽  
pp. 241-247
Author(s):  
Silvina N. Valdez ◽  
Anabel Villalba ◽  
Ruben F. Iacono ◽  
Edgardo Poskus
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Acid Decarboxylase ◽  
Diabetic Patients ◽  
Adult Onset ◽  
First Line ◽  
Single Tube ◽  
Tube Test

scholarly journals No Specific Reactivity to E. Coli Glutamic Acid Decarboxylase from Sera of Newly-Diagnosed Insulin Dependent Diabetic Patients

2003 ◽  
Vol 16 (2) ◽  
pp. 129-138
Author(s):  
C. Konidaris ◽  
P.G. Mitlianga ◽  
G.K. Papadopoulos
Keyword(s):  
Monoclonal Antibody ◽  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Solid Phase ◽  
Normal Subjects ◽  
Cross Reactivity ◽  
Acid Decarboxylase ◽  
Diabetic Patients ◽  
Dependent Manner ◽  
E Coli

The 65 kD isoform of Glutamic Acid Decarboxylase (GAD), is one of the major autoantigens in human type 1 diabetes mellitus. This enzyme shares aminoacid identity, in select regions already determined as antigenic with its counterpart from E. coli. We tested the reactivity of diabetic and normal sera and an E. coli GAD-specific monoclonal antibody (2D9) to E. coli GAD by solid phase and competition ELISA, as well as immunoblotting to check for cross-reactivity of autoantibodies to the two antigens. Specific antibodies for E. coli GAD are present in diabetics and normal subjects without any differences in frequency and titer. The reactivity of such antibodies in ELISA could be blocked in a dose-dependent manner by the addition of excess antigen in the liquid phase. Furthermore, the monoclonal antibody against E. coli GAD does not recognise human recombinant GAD65 in an ELISA. We conclude that there is no basis for cross-reactivity between the two antigens, and antibody reactivity to GAD65 in man cannot arise from cross-reactivity to the E. coli enzyme.

Evidence of autoantibodies to glutamic acid decarboxylase in oral fluid of type 1 diabetic patients

2002 ◽  
Vol 57 (3) ◽  
pp. 171-177 ◽  
Author(s):  
Alan Leslie Todd ◽  
Wai-Yoong Ng ◽  
Yung Seng Lee ◽  
Kah Yin Loke ◽  
Ah Chuan Thai
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Oral Fluid ◽  
Acid Decarboxylase ◽  
Diabetic Patients ◽  
Type 1 Diabetic Patients

Clinical and genetic characteristics of diabetic patients with high-titer (>10,000U/ml) of antibodies to glutamic acid decarboxylase

2005 ◽  
Vol 99 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Michiyo Ishii ◽  
Goji Hasegawa ◽  
Michiaki Fukui ◽  
Hiroshi Obayashi ◽  
Mitsuhiro Ohta ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
High Titer ◽  
Acid Decarboxylase ◽  
Diabetic Patients ◽  
Genetic Characteristics
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