Genomic integration of high-risk human papilloma virus in the nucleus of
cervical epithelial mucosal cells leads to epithelial dysplasia. The aim of
this study was to determine the relevance of correlation between epithelial
survivin expression and the degree of human papilloma virus (HPV)-induced
cervical epithelial dysplasia, and to establish the significance of
morphometric analysis of the nuclear area in the assessment of the degree of
cervical dysplasia. This retrospective study included 99 women with primary,
previously untreated lesions, and colposcopic findings indicating dysplasia,
in whom a cytological test by Papanicolaou method was interpreted according
to the Bethesda criteria as lowgrade squamous intraepithelial lesion (LSIL),
high-grade squamous intraepithelial lesion (HSIL), and atypical squamous
cells of undetermined significance (ASCUS). We performed human papilloma
virus (HPV) typing by PCR for evidence of viruse types 16, 18, 31, 33. After
biopsy of the cervical mucosa, we performed hematoxylin-eosin (H-E) and
Periodic Acid Schiff (PAS) staining, and immunohistochemical and morphometric
analysis of tissue samples. The control group consisted of 12 women without
dysplasia and without a verified infection of cervical high-risk HPV. A high
statistical correlation between the degree of dysplasia and expression of
survivin was found in patients with different types of cervical dysplasia (p
= 0.003). We observed a high statistical difference between the area of
nuclei at different degrees of cervical dysplasias (p = 0.000). The
high-grade cervical dysplasia had a more than 2-fold higher level of ranking
in comparison to low-grade dysplasia, and a more than 10-fold higher ranking
than the control group without cervical dysplasia.
Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia
