A synopsis of methods of sweat tests in pathology

2014 ◽  
Vol 47 (9) ◽  
pp. 757-758 ◽  
Author(s):  
Paul M. Quinton
Keyword(s):  
Sweat Tests

Micro-flowcell conductometric sweat analysis for cystic fibrosis diagnosis

2000 ◽  
Vol 37 (3) ◽  
pp. 399-407 ◽  
Author(s):  
H Lewis Webster ◽  
Carmelo G Quirante
Keyword(s):  
Power Supply ◽  
Time Interval ◽  
Sweating Rate ◽  
Sensor Cell ◽  
Sweat Testing ◽  
Small Holders ◽  
Flow Through ◽  
To Receive ◽  
Conductivity Cell ◽  
Sweat Tests

This paper describes a device specifically designed to facilitate neonatal sweat testing. The components are sized appropriately for attachment to the limbs of newborns. Iontophoretic electrodes, with pilocarpine gel inserts, are latched into small holders attached by straps to the limb. The holder at the anodic site remains in place to receive and align the sensor cell, which uses a conical collecting surface to channel the sweat directly and anaerobically from the sweat ducts to the continuous flow-through conductivity cell within its body. A crib-side analysis unit incorporates an iontophoretic power supply and displays a continuous readout of sweat electrical conductivity. The average conductivity during a specific time interval and the initial sweating rate are automatically displayed. The method, which simplifies sweat tests, is currently being assessed in three neonatal clinical trials to test its ability to reduce test failures in the newborn due to insufficient sweat.

scholarly journals Disposition of MDMA and Metabolites in Human Sweat Following Controlled MDMA Administration

2009 ◽  
Vol 55 (3) ◽  
pp. 454-462 ◽  
Author(s):  
Allan J Barnes ◽  
Bruno S De Martinis ◽  
David A Gorelick ◽  
Robert S Goodwin ◽  
Erin A Kolbrich ◽  
...  
Keyword(s):  
Solid Phase ◽  
Random Order ◽  
Research Unit ◽  
Sweat Test ◽  
Test Results ◽  
Double Blind ◽  
Scientific Database ◽  
Sweat Testing ◽  
Clinical Research Unit ◽  
Sweat Tests

Abstract Background: Understanding the excretion of 3,4-methylenedioxymethamphetamine (MDMA) and metabolites in sweat is vital for interpretation of sweat tests in drug treatment, criminal justice, and workplace programs. Methods: Placebo, low (1.0 mg/kg), and high (1.6 mg/kg) doses of oral MDMA were given double-blind in random order to healthy volunteers (n = 15) with histories of MDMA use. Participants resided on the closed clinical research unit for up to 7 days after each dose. Volunteers wore PharmChek® sweat patches (n = 640) before, during, and after controlled dosing. Patches were analyzed by solid phase extraction and GC-MS for MDMA, methylenedioxyamphetamine (MDA), 4-hydroxy-3-methoxyamphetamine (HMA), and 4-hydroxy-3-methoxymethamphetamine (HMMA). Limits of quantification (LOQ) were 2.5 ng/patch for MDMA and 5 ng/patch for HMA, HMMA, and MDA. Results: MDMA was the primary analyte detected in 382 patches (59.7%), with concentrations up to 3007 ng/patch. MDA was detected in 188 patches (29.4%) at <172 ng/patch, whereas no HMMA or HMA was detected; 224 patches (35.0%) and 60 patches (9.4%) were positive for MDMA and MDA, respectively, at the 25-ng/patch threshold proposed by the Substance Abuse and Mental Health Services Administration. Conclusions: Sweat testing was shown to be an effective and reliable method for monitoring MDMA use in this controlled MDMA administration study. However, variability in sweat excretion suggests that results should be interpreted qualitatively rather than quantitatively. These data provide a scientific database for interpretation of MDMA sweat test results.

scholarly journals Clinical practices for intermediate sweat tests following abnormal cystic fibrosis newborn screens

2011 ◽  
Vol 10 (6) ◽  
pp. 460-465 ◽  
Author(s):  
Megan R. Nelson ◽  
Craig R. Adamski ◽  
Audrey Tluczek
Keyword(s):  
Cystic Fibrosis ◽  
Clinical Practices ◽  
Sweat Tests

SWEATING IN CONGENITAL HEART DISEASE

PEDIATRICS ◽  
1968 ◽  
Vol 41 (1) ◽  
pp. 123-129
Author(s):  
Blanche P. Alter ◽  
Emily E. Czapek ◽  
Richard D. Rowe
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
Congenital Heart Disease ◽  
Heart Disease ◽  
Congenital Heart ◽  
Sweat Test ◽  
Endocardial Fibroelastosis ◽  
History Of Or ◽  
History Of ◽  
Sweat Tests

Sweating was found to be increased in children with congenital heart disease who had a propensity to congestive heart failure, e.g., children with endocardial fibroelastosis or large or moderate sized left-to-right shunts. This was suggested in a review of cardiac clinic records of 220 patients and was supported by the results of pilocarpine sweat tests which were performed on 34 cardiac patients. By history and by measurement of the amount of sweat produced, children with a history of or tendency toward heart failure could be predicted though patients did not need to be in failure when tested. Contrary to previous opinion, the left-to-right shunt was not in itself sufficient to cause the child to sweat. The shunt had to be large enough to be associated with failure at some time. It is suggested that the pilocarpine sweat test might actually be useful as an aid in predicting a child's potential for heart failure. Several theories regarding the mechanism of sweating in these situations are discussed.

Assessment of Sweat-Testing Practices for the Diagnosis of Cystic Fibrosis

2001 ◽  
Vol 125 (11) ◽  
pp. 1420-1424 ◽  
Author(s):  
Vicky A. LeGrys
Keyword(s):  
Clinical Laboratory ◽  
Data Extraction ◽  
National Committee ◽  
Data Synthesis ◽  
Study Selection ◽  
Erroneous Result ◽  
Sweat Testing ◽  
Data Source ◽  
Areas Of Concern ◽  
Sweat Tests

Abstract Objective.—To describe the results of the College of American Pathologists survey questions assessing the current practice of sweat testing in North America and to identify areas in which improvement is needed. Data Source.—Results of the supplemental questions to the SW-B 2000 survey. Study Selection.—Supplemental questions were designed to assess variation in sweat collection, analysis, and interpretation. Data Extraction.—Extractions of the data were made based on the relevance of the data to the objectives of the review. Data Synthesis.—The majority of laboratories surveyed performed sweat testing according to the procedures described in the National Committee for Clinical Laboratory Standards' document. The study revealed that a number of laboratories have adopted poor practice standards and are potentially compromising patient care. Areas of concern include the number of laboratories performing few sweat tests per year, the persistence of unreliable methodology, misunderstanding of collection parameters, lack of patient education, and erroneous result reporting. Conclusions.—The study identified areas of concern toward which educational efforts can be directed. Such efforts include the development of a College of American Pathologists accreditation checklist for sweat testing and targeted responses in the sweat analysis participant summary report.

Sweat Tests in Patients with Diabetes Insipidus

1988 ◽  
Vol 27 (7) ◽  
pp. 330-332 ◽  
Author(s):  
Eileen Doherty-Fuller ◽  
Kenneth C. Copeland
Keyword(s):  
Diabetes Insipidus ◽  
Patients With Diabetes ◽  
Sweat Tests

scholarly journals Sweat tests in the newborn period.

1973 ◽  
Vol 48 (4) ◽  
pp. 316-318 ◽  
Author(s):  
J D Hardy ◽  
S H Davison ◽  
M U Higgins ◽  
P N Polycarpou
Keyword(s):  
Newborn Period ◽  
Sweat Tests

Cystic fibrosis and celiac disease - multifaceted and similar

Folia Medica ◽  
2013 ◽  
Vol 55 (3-4) ◽  
pp. 87-89 ◽  
Author(s):  
Nelly D. Genkova ◽  
Ivan V. Yankov ◽  
Miroslava N. Bosheva ◽  
Benjamin L. Anavi ◽  
Dafina G. Grozeva ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Celiac Disease ◽  
Chronic Diarrhea ◽  
Serological Test ◽  
Clinical Manifestations ◽  
Duodenal Mucosa ◽  
Gluten Free ◽  
Immunological Tests ◽  
Targeted Screening ◽  
Sweat Tests

ABSTRACT Celiac disease and cystic fibrosis share a number of clinical manifestations. The comorbidity rate of these diseases is low: 1:200000. We present a case of a child aged 1 year and 5 months, born to a mixed-marriage parents, with concomitant cystic fibrosis and celiac disease manifesting initially with chronic diarrhea. Diagnosis of cystic fibrosis was made on the basis of changes in pulmonogram and three positive sweat tests with the malabsorption managed. Celiac disease was demonstrated through immunological tests (serological test of anti-transglutaminase antibodies of IgA class), histological tests (altered duodenal mucosa) and the therapeutic effect of a gluten-free diet. This case is the first ever reported case of a child with concomitant cystic fibrosis and celiac disease in Bulgaria. The case suggests the need for targeted screening for celiac disease in children with cystic fibrosis.

Sign in / Sign up

Export Citation Format

Share Document