Effect of Morphine and Bupivacaine on Nicotine-Induced Catecholamine Secretion from Encapsulated Chromaffin Cells

2005 ◽  
Vol 277-279 ◽  
pp. 56-61
Author(s):  
Hai Yan Hong ◽  
Jeong Ok Lim ◽  
Woon Yi Baek
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cancer Patients ◽  
Chromaffin Cells ◽  
High Performance ◽  
Hplc Analysis ◽  
Intractable Pain ◽  
Analgesic Effects ◽  
Bovine Chromaffin Cells ◽  
Analysis System

The control of intractable pain through transplanted of chromaffin cells has been recently reported where the analgesic effects are principally due to the production of opioid peptides and catecholamines (CAs) by the chromaffin cells. Currently many cancer patients receive general opioids or local anesthetics, such as bupivacaine. Therefore, the present study investigated the effect of morphine or bupivacaine on the secretion of nicotine-induced CAs from encapsulated chromaffin cells over a period of 180 min. As such, bovine chromaffin cells were isolated and encapsulated with alginate–poly–L–lysine–alginate (APA) biomaterials to prevent immunorejection. The capsules were then pre-incubated with nicotine for 5 min prior to morphine or bupivacaine stimulation, and the quantity of CAs analyzed using a high performance liquid chromatography (HPLC) analysis system. The resulting data showed that the encapsulated chromaffin cells retained the ability of their parent chromaffin cells when responding to opioids by suppressing the release of CAs. In contrast, bupivacaine did not have any statistically significant affect on the basal and nicotine-induced CA release from the encapsulated chromaffin cells.

HPLC Analysis of Oxytetracycline Residues in Honey

1986 ◽  
Vol 49 (5) ◽  
pp. 383-388 ◽  
Author(s):  
PETER SPORNS ◽  
SUET KWAN ◽  
LAWRENCE A. ROTH
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aqueous Solutions ◽  
High Performance ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Hplc Method ◽  
Limits Of Detection ◽  
Ph Values ◽  
Double Extraction

Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.

ANTHOCYANIN, AGLYCONE AND AGLYCONE-SUGAR CONTENT OF FRUITS OF TEMPERATE NORTH AMERICAN SPECIES OF Gaylussacia (ERICACEAE)

1988 ◽  
Vol 68 (1) ◽  
pp. 247-253 ◽  
Author(s):  
J. R. BALLINGTON ◽  
W. E. BALLINGER ◽  
E. P. MANESS
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
North American ◽  
High Performance ◽  
Hplc Analysis ◽  
Sugar Content ◽  
North American Species ◽  
The Other ◽  
Phylogenetic Implications

HPLC analysis of the true huckleberry species Gaylussacia baccata, G. dumosa, G. frondosa, G. mosieri, and G. ursina identified the 3-monoarabinosides, 3-monogalactosides, and 3-monoglucosides of cyanidin, delphinidin, malvidin, peonidin, and petunidin. Gaylussacia brachycera contained all anthocyanins, except peonidin-3-arabinoside. Gaylussacia brachycera differed from other species in percent delphinidin-3-arabinoside. It was higher than the other species in percent of the aglycone delphinidin and lower in cyanidin, and also higher in percent of the sugar arabinose. There were no detectable differences among the other species for anthocyanins, aglycones, or aglycone-sugars. The phylogenetic implications of the similarities among species of Gaylussacia and Vaccinium in anthocyanins, aglycones, and aglycone-sugars of the fruit were discussed.Key words: High-performance liquid chromatography, huckleberries, blueberries, chemotaxonomy, taxonomy, biosystematics

Determination of Doxorubicin and Doxorubicinol in Plasma of Cancer Patients by High-Performance Liquid Chromatography

1999 ◽  
Vol 266 (2) ◽  
pp. 216-221 ◽  
Author(s):  
Peter de Bruijn ◽  
Jaap Verweij ◽  
Walter J. Loos ◽  
Herman J. Kolker ◽  
André S.T. Planting ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cancer Patients ◽  
High Performance

Effects of Baicalein on Prostanoid Generation from the Lung and Contractile Responses of the Trachea in Guinea Pig

1997 ◽  
Vol 25 (01) ◽  
pp. 37-50 ◽  
Author(s):  
Koji Miyamoto ◽  
Takeshi Katsuragi ◽  
Parhat Abdu ◽  
Tatsuo Furukawa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Smooth Muscle ◽  
Liquid Chromatography ◽  
Guinea Pig ◽  
High Performance ◽  
Hplc Analysis ◽  
Antigen Challenge ◽  
Cyclooxygenase Inhibitor ◽  
Prostaglandin F ◽  
Stimulation Of

Effects of baicalein on release of slow reacting substance of anaphylaxis (SRS-A) or leukotriene (L T) from the sensitized guinea pig lung after antigen challenge and tonus of guinea pig tracheal muscles were studied. Baicalein inhibited release of SRS-A from sensitized guinea pig lung after antigen challenge. High-performance liquid chromatography (HPLC) analysis revealed that released SRS-A consisted of LTC 4 and D 4. Baicalein also reduced release of LTC 4 and D 4 from the sensitized lung after antigen challenge. Baicalein relaxed the isolated guinea pig tracheal smooth muscle contracted by LTD 4, carbachol or histamine. However, this compound produced a contraction when the tracheal muscle was contracted by prostaglandin F 2α ( PGF 2α). This contraction by baicalein was abolished by pretreatment with indomethacin, a cyclooxygenase inhibitor. Baicalein elicited a relaxation in the normal non-sensitized tracheal preparation but a contraction in the tissue isolated from actively sensitized guinea pig in 4 among 7 cases. Baicalein also produced a contraction in the trachea pretreated with phorbol dibutyrate and contracted by carbachol, which was eliminated after treatment with indomethacin. The results suggest that baicalein exerts action via, at least, two different mechanisms, the inhibition of releasing SRS-A (LTs) and direct relaxing effects on the trachea. Besides, baicalein seems to produce contraction under certain conditions, which may involve stimulation of the cyclooxygenase pathway.

Development of control material for hemoglobin analysis

2012 ◽  
Vol 50 (2) ◽  
Author(s):  
Sakorn Pornprasert ◽  
Kunyakan Kongthai ◽  
Jarurin Waneesorn ◽  
Kanokwan Jaiping ◽  
Kallayanee Treesuwan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Freeze Drying ◽  
Sucrose Solution ◽  
Control Material ◽  
Normal Individuals ◽  
Thalassemia Trait

AbstractThere is no certified control material for hemoglobin analysis which has the hemoglobin (Hb)AWashed and dialysed erythrocytes of normal individuals and patients with β-thalassemia trait, HbE trait, β-thalassemia/HbE disease, homozygous HbE were lysed in 5% sucrose solution. The lyophilized hemoglobin control materials were prepared by using a freeze-drying (lyophilization) method. The high performance liquid chromatography (HPLC) analysis of lyophilized hemoglobin was performed after storing at –20°C for 1, 15 and 30 days and for 3 months.The chromatograms of lyophilized hemoglobin control materials showed similar patterns and similar levels of HbA, HbAThe lyophilized hemoglobin could be developed and used as control materials for hemoglobin analysis.

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