Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions

The fungal biocontrol agent, Trichoderma harzianum, was evaluated for its potential to control the root-knot nematode Meloidogyne javanica. In greenhouse experiments, root galling was reduced and top fresh weight increased in nematode-infected tomatoes following soil pretreatment with Trichoderma peat-bran preparations. The use of a proteinase Prb1-transformed line (P-2) that contains multiple copies of this gene improved biocontrol activity in the greenhouse experiments compared with the nontransformed wild-type strain (WT). All the Trichoderma strains showed the ability to colonize M. javanica-separated eggs and second-stage juveniles (J2) in sterile in vitro assays, whereas P-2 also penetrated the egg masses. This protease-transformed line presented the same nematicidal and overall proteolytic activity as the WT in in vitro tests in which concentrated soil extracts from Trichoderma-treated soils immobilized the infective J2. However, the J2 immobilization and proteolytic activities of both P-2 and the WT were higher than those obtained with strain T-203. Characterization of the activity of all Trichoderma strains soil extracts on J2 showed that it was heat resistant and restricted to the low-molecular-weight fraction (less than 3 kDa). It is suggested that improved proteolytic activity of the antagonist may be important for the biological control of the nematodes.

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The Development of a Technique for Studying the Host/Parasite Relationship of the Root-Knot Nematode Meloidogyne Incognita Under Controlled Conditions

Nematologica ◽

10.1163/187529259x00354 ◽

1959 ◽

Vol 4 (1) ◽

pp. 43-55 ◽

Cited By ~ 27

Author(s):

F.C. Peacock

Keyword(s):

Meloidogyne Incognita ◽

Root Knot Nematode ◽

Controlled Conditions ◽

Host Parasite Relationship ◽

Parasite Relationship ◽

Host Parasite ◽

Relationship Of

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External secretions from the false root-knot nematode, Nacobbus aberrans

Nematologica ◽

10.1163/005425998x00099 ◽

1998 ◽

Vol 44 (3) ◽

pp. 326-330 ◽

Cited By ~ 1

Author(s):

K. Evans ◽

J. Rowe ◽

N. VON MEN-DE ◽

M. Gravato-Nobre ◽

R.H. MANZANILLA-LόPEZ

Keyword(s):

Root Knot Nematode ◽

Nacobbus Aberrans

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Evaluation of bacterial biological control agents for control of root-knot nematode disease on tomato

Crop Protection ◽

10.1016/j.cropro.2015.12.009 ◽

2016 ◽

Vol 84 ◽

pp. 8-13 ◽

Cited By ~ 25

Author(s):

Lihong Zhou ◽

Gary Yuen ◽

Yang Wang ◽

Lanfang Wei ◽

Guanghai Ji

Keyword(s):

Biological Control ◽

Biological Control Agents ◽

Root Knot Nematode

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Bakteri Endofit asal Tanaman Tembakau sebagai Agens Pengendali Meloidogyne spp.

Jurnal Fitopatologi Indonesia ◽

10.14692/jfi.14.6.215 ◽

2019 ◽

Vol 14 (6) ◽

pp. 215 ◽

Cited By ~ 1

Author(s):

Isnainy dinul Mursyalatiyus ◽

Abdul Munif ◽

Abdjad Asih Nawangsih

Keyword(s):

Biological Control ◽

Endophytic Bacteria ◽

Biological Control Agent ◽

Tobacco Plant ◽

Control Agent ◽

Root Knot Nematode ◽

Tobacco Plants ◽

Physiological Characterization ◽

Protease Enzyme ◽

Meloidogyne Spp

Endophytic Bacteria from Tobacco Plant as Biocontrol Agent of Meloidogyne spp.Soilborne disease on tobacco plants caused by fungal and bacterial infection in association with root-knot nematode (Meloidogyne spp.) may cause significant yield loss. Endophytic bacteria have been recognized as biological control agent for Meloidogyne spp. as well as plant promoting growth agent. Research was conducted to evaluate endophytic bacteria isolated from tobacco plants as biological control agent for Meloidogyne spp. infecting tobacco. A total of 215 isolates of endophytic bacteria were isolated from root of two tobacco varieties, Kemloko and Prancak 95. Biosafety screening showed that 80 isolates (37%) and 7 isolates (8%) gave negative reaction on hypersensitivity test and hemolysis test, respectively. Seven isolates i.e. TPT3.10, TPT2.1, TK3n8, TK2t21, TK2n8, TK3n1 and TK2t11 were able to promote plant growth and increase the mortality of juvenile Meloidogyne spp. Physiological characterization of endophytic bacteria showed that most of the isolates were able to produce protease enzyme, phosphate, nitrogen and HCN. The same isolates were also able to suppress the number of galls from 80.09% up to 93.82%. Two isolates, TPT3.10 and TK2n8, are considered having the best suppression on root gall formation.

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