Investigation for zoonotic disease pathogens (Aeromonas hydrophila, Pseudomonas fluorescens, Streptococcus iniae) seen in carp farms in Duhok region of Northern Iraq by molecular methods

2017 ◽  
Author(s):  
Kamiran Abdulrahman Mohammed ◽  
Muhammed Arabacı ◽  
Şükrü Önalan
Keyword(s):  
Pseudomonas Fluorescens ◽  
Aeromonas Hydrophila ◽  
Zoonotic Disease ◽  
Molecular Methods ◽  
Streptococcus Iniae ◽  
Northern Iraq

scholarly journals Haemato-immunological responses and effectiveness of feed-based bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila infections in hybrid red tilapia (Oreochromis spp.)

2020 ◽  
Author(s):  
Md. Shirajum Monir ◽  
Md Sabri bin Mohd Yusoff ◽  
Zarirah binti Mohamed Zulperi ◽  
Hasliza binti Abu Hassim ◽  
Aslah Mohamad ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Post Infection ◽  
Serum Antibody ◽  
Economic Losses ◽  
Control Group ◽  
Streptococcus Iniae ◽  
Post Vaccination ◽  
Bivalent Vaccine ◽  
Red Tilapia ◽  
Immunological Responses

Abstract Background Streptococcosis and Motile Aeromonad Septicemia (MAS) are important diseases of tilapia, Oreochromis spp. and causes huge economic losses in aquaculture globally. The feed-based vaccination may be an alternative to minimize major infectious diseases in tilapia. Thus, this study aims to evaluate the haemato-immunological responses and effectiveness of a newly developed feed-based killed bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila in hybrid red tilapia. A total of 495 hybrid red tilapia of 61.23±4.95 g were distributed into 5 groups (each with triplicate). The fish were immunized orally through bivalent (combined S. iniae and A. hydrophila ) spray vaccine (BS group), bivalent formulate vaccine (BF group), monovalent S. iniae vaccine (MS group), monovalent A. hydrophila vaccine (MA group) and unvaccinated as a control group. The vaccine was orally administered on days 0, 14 and 42 applied feed-based bacterin at 5% body weight. The blood and spleen samples were collected from all groups on 7, 21 and 49 days post-vaccination, and also 96 hours post-infection to assess their haemato-immune responses. Results Compared with the unvaccinated group, leukocyte, lymphocytes, monocytes, granulocytes counts in vaccinated groups were significantly ( P < 0.05) increased on 21, 49 days post-vaccination and also 96 hours post-infection, while erythrocytes, haemoglobin and haematocrit in vaccinated groups were significantly ( P < 0.05) enhanced only 96 hours post-infection. Additionally, the lysozyme and phagocytic activity and, serum antibody (IgM) were significantly higher ( P < 0.05) against S. iniae and A. hydrophila in vaccinated groups compared to the unvaccinated group in the pre- and post-infection. Results from the challenge through co-infection with S. iniae and A. hydrophila showed the relative percent survival (RPS) in BF group was 76.37±5.14%, which had the capacity to induce significant protection ( P < 0.05) compared to others groups. Conclusions This study demonstrates the bivalent formulate (BF) group could elicit significant non-specific and specific immunological responses with higher protection in hybrid red tilapia. In addition, this newly developed feed-based bivalent vaccination can be a promising technique for effective and large scale fish immunization in the aquaculture industry.

scholarly journals Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus)

F1000Research ◽  
2019 ◽  
Vol 7 ◽  
pp. 1847 ◽  
Author(s):  
Esti Handayani Hardi ◽  
Rudy Agung Nugroho ◽  
Irawan Wijaya Kusuma ◽  
Wiwin Suwinarti ◽  
Agung Sudaryono ◽  
...  
Keyword(s):  
Survival Rate ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Prevention Trial ◽  
Phagocytic Index ◽  
Immunological Parameters

Background: The combination of some plant extracts to prevent and treat bacterial infections is gaining momentum, because of effectiveness against certain bacteria. This study aims to describe the antibacterial and immunostimulant abilities of Boesenbergia pandurata (BP), Solanum ferox (SF) and Zingiber Zerumbet (ZZ) plant extracts to treat and prevent Aeromonas hydrophila and Pseudomonas fluorescens infection on Tilapia (Oreochromis niloticus). Methods: Tilapia (initial weight 15±2 g) were injected intramuscularly (0.1 ml/fish) with a combination of A. hydrophila and P. fluorescens at a density of 1×10 5 CFU ml -1 of each bacteria. Treatment trials were performed at day 7 post-injection with each combined extract, while the prevention trial was performed by including the combined extract into the commercial diet for six and seven days prior to injection. Various extract combinations were 60 mg SF extract/kg feed with 40 mg ZZ/kg feed (SF60/ZZ40), SF50/ZZ50, BP90/SF10, and BP50/SF50. Haemato-immunological parameters were performed for four weeks. Results: In prevention trials, tilapia fed SF50/ZZ50 showed a significant increase of white and red blood cells. Similarly, significantly increased haematocrit was found in tilapia fed SF50/ZZ50 in the treatment trial but not in the prevention trial. In both trials, haemoglobin of tilapia was not affected by any combined extracts but decreased the number of bacteria. Phagocytic index, respiratory burst, lysozyme activity and survival rate of fish fed combined extracts were found significantly higher than controls. The amount of pathogenic bacteria in fish fed combined extracts was lower than the control at week 4 (P<0.05). In both trials The percentage of survival rate and relative percent survival of tilapia fed SF 50/ZZ 50, showed the optimum results compared to the other combinations. Conclusions: The combined extract in feed, especially SF50/ZZ50 has a positive effect on the tilapia's innate immune system of tilapia to treat and prevent bacterial infections.

scholarly journals Screening of Probiotic Bacteria from Intestine and Culture Environment of Hoeven’s slender carp Leptobarbus hoeveni Blkr to Control Pathogenic Bacteria

2010 ◽  
Vol 9 (2) ◽  
pp. 127
Author(s):  
. Sunarto ◽  
. Sukenda ◽  
. Widanarni
Keyword(s):  
Aeromonas Hydrophila ◽  
Pathogenic Bacteria ◽  
Probiotic Bacteria ◽  
Streptococcus Iniae ◽  
Mycobacterium Fortuitum ◽  
Fish Pathogen ◽  
Culture Environment ◽  
Control Disease

The ability of probiotic bacteria to control disease infection has been used in aquaculture. This experiment was conducted to isolate and characterize probiotic bacteria; the competition test its ability probiotic bacteria against pathogenic bacteria; and to improve survival rate of Leptobarbus hoeveni. The bacteria were isolated from Leptobarbus hoeveni and its culture environment, and then tested to know its ability to inhibit bacterial fish pathogen in-vitro. Furthermore, the selected probiotic bacteria were tested in vivo to evaluate their ability to inhibit pathogen of Leptobarbus hoeveni.  The result showed that probiotic bacteria inhibit the growth of Streptococcus iniae, Flexibacter columnaris, Mycobacterium fortuitum and Aeromonas hydrophila in vitro.  Isolate DD3 was the best of candidate probiotic because of the ability to inhibit pathogen, especially A. hydrophila, the most virulent bacteria in Leptobarbus hoeveni.<br /><br />Key Words  : probiotic bacteria, Leptobarbus hoeveni, pathogenic bacteria<br /><br />Abstrak<br /><br />Kemampuan bakteri probiotik untuk mengendalikan penyakit infeksi telah digunakan dalam akuakultur. Tujuan penelitian ini adalah mengisolasi dan mengkarakterisasi bakteri probiotik, menguji kemampuan bakteri probiotik terhadap bakteri patogen, sehingga dapat meningkatkan tingkat kelangsungan hidup ikan jelawat. Bakteri diisolasi dari usus ikan jelawat dan lingkungan budaya, kemudian diuji kemampuannya menghambat bakteri patogen secara in-vitro. Selanjutnya bakteri probiotik yang dipilih diuji secara in vivo untuk mengevaluasi kemampuannya dalam menghambat patogen di dalam tubuh ikan jelawat. Dari hasil penelitian diperoleh bakteri probiotik yang diisolasi dari usus dan lingkungan budaya ikan jelawat menunjukkan penghambatan pertumbuhan terhadap Streptococcus iniae, Flexibacter columnaris, Mycobacterium fortuitum dan Aeromonas hydrophila secara in vitro. Isolat DD3 merupakan kandidat probiotik terbaik, karena mempunyai kemampuan untuk menghambat bakteri patogen,  khususnya bakteri  A. hydrophila adalah bakteri yang paling viluren bagi ikan jelawat.<br />    <br />Kata Kunci:   bakteri probiotik, ikan jalawat dan baktri patogen<br />

scholarly journals Efficacy of Aqueous Extract of Pistia Stratiotes (Water Lettuce) Against some Tropical Fish Bacterial

2021 ◽  
Author(s):  
Adelakun Kehinde Moruff ◽  
Halidu Shafiu Kilishi ◽  
Fajobi Enobong Aloysious ◽  
Joshua Deji Abiodun
Keyword(s):  
Pseudomonas Fluorescens ◽  
Aqueous Extract ◽  
Aeromonas Hydrophila ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Tropical Fish ◽  
Pistia Stratiotes ◽  
Significant Activity ◽  
Water Lettuce ◽  
Fish Pathogens

Background: The study was undertaken to determine the phytochemical screening and the antibacterial activities of Pistia stratiotes (water lettuce) against some clinically isolated fish pathogens. Methods: The healthy and disease free plants samples were collected from Jebba Upper Basin of River Niger and tested for their antibacterial activities and phytochemical properties. Pistia stratiotes aqueous extract was extracted using sterile distilled water. The Agar diffused methods were used to determine the antimicrobial actions of the plant against fish pathogens of Pseudomonas fluorescens, Escherichia coli, Salmonella typhi, Aeromonas hydrophila, and Vibrio anguillarum. Results: The antibacterial investigation of aqueous extracts revealed the significant activity against these bacterial. Moreover, phytochemical examination of the crude extract showed the occurrence of saponins, alkaloids, tannins, anthraquinones, flavonoids, and catechins, which completely inhibited the growth of V. anguillarum and E. coli. However, Salmonella typhi, Pseudomonas fluorescens, and Aeromonas hydrophila exhibited moderate varying levels of resistance. Conclusion: The finding of this study showed that bioactive resistance from Pistia stratiotes can help disrupt the activities of various fish bacterial in aquaculture practices

scholarly journals Aktivitas Antagonis Bacillus subtilis terhadap Streptococcus iniae dan Pseudomonas fluorescens (ANTAGONIST ACTIVITY OF BACILLUS SUBTILIS AGAINST STREPTOCOCCUS INIAE AND PSEUDOMONAS FLUORESCENS)

2017 ◽  
Vol 18 (3) ◽  
pp. 409
Author(s):  
Budianto Budianto ◽  
Heny Suprastyani
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Fish Farming ◽  
Inhibition Zone ◽  
Probiotic Bacteria ◽  
Streptococcus Iniae ◽  
Antagonist Activity ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Paper Disc

Intensification of fish farming has caused various impacts, for example diseases in fish. One of the diseases in fish is a bacterial disease. The use of probiotic bacteria as an antimicrobial agent, which is relatively safe and effective, is a strategy to treat the disease. Bacillus subtilis is probiotic bacteria which can produce bacteriocin compounds and has antagonistic effects against both Gram negative and Gram positive bacteria. The aim of this study was to evaluate the antagonist activity of B. subtilis against Streptococcus iniae and Pseudomonas fluorescens. Antagonist activity test was done by using paper disc diffusion against the bacteria. The variations on the test paper disc method used were based on the difference of B. subtilis incubation time, such as: 0, 6, 12, 18, 24 and 30 hours. The results showed B. subtilis (generation time = 30.62 min) produced antibacterial compounds which inhibited the growth of the bacteria. Antagonist activity was detected in early exponential phase, six hours, with inhibition zone diameter of 7.28 ± 0.18 mm (S. iniae) and 6.75 ± 0.11 mm (P. fluorescens) and reached optimum at early stationary phase, 24 hours, the inhibition zone diameter of 8.84 ± 0.28 mm (S. iniae) and 9.14 ± 0.91 mm (P. fluorescens). It can be concluded that B. subtilis can contribute in controlling the spread of bacterial diseases in fish farming, particularly caused by S. iniae and P. fluorescens ABSTRAK Intensifikasi budidaya ikan telah menyebabkan berbagai dampak, seperti penyakit pada ikan. Salah satunya adalah penyakit bakteriawi. Penggunaan bakteri probiotik sebagai agen antimikrob yang aman dan efektif adalah salah satu strategi untuk menanggulangi penyakit tersebut. Bacillus subtilis merupakan bakteri probiotik yang dapat memproduksi senyawa bakteriosin dan memiliki efek antagonis terhadap bakteri Gram negatif dan positif. Tujuan dari penelitian ini adalah untuk mengevaluasi aktivitas antagonis dari B. subtilis terhadap Streptococcus iniae dan Pseudomonas fluorescens. Uji aktivitas antagonis dilakukan dengan menggunakan difusi cakram kertas terhadap bakteri uji. Variasi perlakuan pada uji cakram kertas adalah menggunakan perbedaan waktu inkubasi B. subtilis, yaitu waktu inkubasi 0, 6, 12, 18, 24 dan 30 jam. Berdasarkan hasil penelitian, B. subtilis (waktu generasi=30,62 menit) dapat memproduksi senyawa antibakteri yang dapat menghambat pertumbuhan bakteri uji. Aktivitas antagonis terdeteksi pada awal fase eksponensial yaitu inkubasi enam jam, dengan diameter zona hambat sebesar 7,28 ± 0,18 mm (S. iniae) dan 6,75 ± 0,11 mm (P. fluorescens) dan mencapai optimum pada awal fase stasioner yaitu inkubasi 24 jam, dengan diameter zona hambat sebesar 8,84 ± 0,28 mm (S. iniae) dan 9,14 ± 0,91 mm (P. fluorescens). Dapat disimpulkan bahwa B. subtilis dapat berperan dalam pengendalian penyebaran penyakit bakteri pada budidaya ikan, khususnya yang disebabkan oleh S. iniae dan P. fluorescens.

scholarly journals Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus)

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1847 ◽  
Author(s):  
Esti Handayani Hardi ◽  
Rudy Agung Nugroho ◽  
Irawan Wijaya Kusuma ◽  
Wiwin Suwinarti ◽  
Agung Sudaryono ◽  
...  
Keyword(s):  
Immune System ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Prevention Trial ◽  
Immunological Parameters ◽  
Prevention Trials

Background: This study aims to describe the antibacterial and immunostimulant abilities of Boesenbergia pandurata (BP), Solanum ferox (SF) and Zingiber Zerumbet (ZZ) plant extracts to treat and prevent Aeromonas hydrophila and Pseudomonas fluorescens infection on Tilapia (Oreochromis niloticus). Methods: Tilapia (initial weight 15±2 g) were injected intramuscularly (0.1 ml/fish) with a combination of A. hydrophila and P. fluorescens at a density of 1×105 CFU ml-1 of each bacteria. Treatment trials were performed at day 7 post-injection with each combined extract, while the prevention trial was performed by including the combined extract into the diet for six and seven days prior to injection. Various combinations of extract—60 ml SF extract/kg feed with 40 ml ZZ/kg feed (SF60/ZZ40), SF50/ZZ50, BP90/SF10, and BP50/SF50—were mixed with a commercial diet and used in both treatment and prevention trials. Haematological and immunological parameters were performed every week for four weeks. Results: In prevention trials, tilapia fed SF50/ZZ50 showed a significant increase of white and red blood cells from weeks 2 to 4. Similarly, significantly increased haematocrit was also found in tilapia fed SF50/ZZ50 in the treatment trial but not in the prevention trial. However, haemoglobin of tilapia in both trials was not affected by any of the various combinations of extract in the diet. Furthermore, phagocytic, respiratory burst, lysozyme activity indexes and survival rate of fish fed with combined extracts were found to be significantly higher than controls. Moreover, the amount of pathogenic bacteria in fish that were fed combined extracts was also lower than the control and was significantly different at week 4. Conclusions: This study indicates that the addition of combined extract into feed has a positive effect on the tilapia's immune system. The SF50/ZZ50 combination appears to improve the innate immune system of tilapia to treat and prevent bacterial infections.

Sign in / Sign up

Export Citation Format

Share Document