Morphologic, cytometric and functional characterization of the common octopus (Octopus vulgaris) hemocytes

The Octopus vulgaris farming is impaired by the high mortality of the paralarvae during the first month of life. Several factors have been investigated in this regard, but no data exist on the body surface mucus, which represents the interface with the outside environment. This study included morphometric analysis and glycoconjugates characterization of skin mucus in reared Octopus vulgaris paralarvae during the first month of life. Four types of mucous cells were distinguished: mucous 1 (m1) and mucous 2 (m2) cells were scattered in the mantle epidermis, mucous 3 (m3) and mucous 4 (m4) in the epithelium surrounding the sucker. Except for the presence of fucosylated and neutral glycoconjugates in all mucous cells, each cell type expressed a characteristic glycopattern. m2 and m4 contained also suphate and acid non-sulphate glycans, m3 lacked suphate glycoproteins. Lectin histochemistry showed that mantle mucous cells (m1, m2) expressed GlcNAc and lactosamine terminating glycans. m2 also contained GalNAc terminal or penultimate to sialic acid. m3 was distinguished by mannosylated glycans terminating with lactosamine and m4 by α2,6 sialoglycans. Glycoproteins terminating with lactosamine, Galβ1,3GalNAc, and α1,6-linked fucose were a common feature of paralarvae surface layer. Morphometry revealed a significant decrease of m1 and m2 abundance during the first month of life, afterwards the reared paralarvae died. Since the glycopattern did not change during the investigated period, the mantle mucous cells abundance could be related to the Octopus vulgaris paralarvae survival.

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Isolation and characterization of Glyceraldehyde-3-phosphate dehydrogenase from the common octopus (Octopus vulgaris Cuvier, 1797)

Reviews in Fish Biology and Fisheries ◽

10.1007/s11160-007-9074-6 ◽

2007 ◽

Vol 18 (3) ◽

pp. 263-271 ◽

Cited By ~ 2

Author(s):

Laila Oukhattar ◽

Tarik Baibai ◽

Adnane Moutaouakkil ◽

Omar Assobhei ◽

Abdelaziz Soukri

Keyword(s):

Octopus Vulgaris ◽

Isolation And Characterization ◽

The Common ◽

Common Octopus

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Molecular and functional characterization of a novel gonadotropin-releasing-hormone receptor isolated from the common octopus (Octopus vulgaris)

Biochemical Journal ◽

10.1042/bj20051615 ◽

2006 ◽

Vol 395 (1) ◽

pp. 125-135 ◽

Cited By ~ 91

Author(s):

Atsuhiro Kanda ◽

Toshio Takahashi ◽

Honoo Satake ◽

Hiroyuki Minakata

Keyword(s):

Functional Characterization ◽

Gonadotropin Releasing Hormone ◽

Brain Extract ◽

Octopus Vulgaris ◽

Peripheral Tissues ◽

Releasing Hormone ◽

Reverse Transcription Pcr ◽

Pcr Products ◽

The Common ◽

Common Octopus

GnRH (gonadotropin-releasing hormone) plays a pivotal role in the regulation of reproduction in vertebrates through interaction with a specific receptor. Previously, we isolated a GnRH homo-logue, oct-GnRH, from the common octopus (Octopus vulgaris). In the present study, we have identified a GnRH receptor (oct-GnRHR) specific for oct-GnRH from Octopus brain. Oct-GnRHR includes domains and motifs typical of vertebrate GnRH receptors. The intron-inserted positions are conserved between oct-GnRHR and the chordate GnRHR genes. The oct-GnRHR expressed in Xenopus (South African clawed frog) oocytes was responsive to oct-GnRH, but not to any other HPLC fractions of the Octopus brain extract. These results show that oct-GnRHR is an authentic receptor for oct-GnRH. Southern blotting of reverse-transcription PCR products revealed that the oct-GnRHR mRNA was widely distributed in the central and peripheral nervous systems and in several peripheral tissues. In situ hybridiz-ation showed that oct-GnRHR mRNA was expressed in some regions involved in autonomic functions, feeding, memory and movement. Oct-GnRH was shown to induce steroidogenesis of testosterone, progesterone and 17β-oestradiol in Octopus ovary and testis, where oct-GnRHR was abundantly expressed. These results suggest that oct-GnRH, like its vertebrate counterparts, acts as a multifunctional neurotransmitter, neuromodulator and hormone-like factor, both in Octopus central nervous system and peripheral tissues, and that both structure and functions of the GnRH family are, at least partially, evolutionarily conserved between octopuses and chordates.

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Shotgun Proteomics Analysis of Saliva and Salivary Gland Tissue from the Common Octopus Octopus vulgaris

Journal of Proteome Research ◽

10.1021/acs.jproteome.8b00525 ◽

2018 ◽

Vol 17 (11) ◽

pp. 3866-3876 ◽

Cited By ~ 5

Author(s):

Legana C. H. W. Fingerhut ◽

Jan M. Strugnell ◽

Pierre Faou ◽

Álvaro Roura Labiaga ◽

Jia Zhang ◽

...

Keyword(s):

Salivary Gland ◽

Shotgun Proteomics ◽

Octopus Vulgaris ◽

Proteomics Analysis ◽

Salivary Gland Tissue ◽

Gland Tissue ◽

The Common ◽

Common Octopus

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Aquaculture potential of the common octopus (Octopus vulgaris Cuvier, 1797): a review

Aquaculture ◽

10.1016/j.aquaculture.2004.05.018 ◽

2004 ◽

Vol 238 (1-4) ◽

pp. 221-238 ◽

Cited By ~ 127

Author(s):

Paulo Vaz-Pires ◽

Pedro Seixas ◽

Alexandra Barbosa

Keyword(s):

Octopus Vulgaris ◽

The Common ◽

Common Octopus

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From injury to full repair: nerve regeneration and functional recovery in the common octopus,Octopus vulgaris

10.1101/693390 ◽

2019 ◽

Author(s):

Pamela Imperadore ◽

Dario Parazzoli ◽

Amanda Oldani ◽

Michael Duebbert ◽

Ansgar Büschges ◽

...

Keyword(s):

Nerve Regeneration ◽

Functional Recovery ◽

Internal Control ◽

Regenerative Process ◽

Full Recovery ◽

Complete Analysis ◽

Octopus Vulgaris ◽

Original Structure ◽

The Common ◽

Common Octopus

AbstractSpontaneous nerve regeneration in cephalopod molluscs occurs in a relative short time after injury, achieving functional recovery of the lost capacities. In particular, transection of the pallial nerve in the common octopus (Octopus vulgaris) determines loss and subsequent restoring of two functions fundamental for survival, i.e. breathing and skin patterning, the latter involved in communication between animals and concealing. The phenomena occurring after lesion have been investigated in a series of previous studies, but a complete analysis of the changes occurring at the level of the axons and the effects on animals appearance during the whole regenerative process is still missing. Our goal is to determine the course of events following injury. Our goal is to determine the course of events following injury, from impairment to full recovery.We observed nerve regeneration, end-target re-innervation and functional reconnections between central brain and periphery, using the contralateral nerve in the same animal as internal control. The final architecture of the regenerated nervous tissue does not mirror the original structure, however functionality returns to match the phenotype of an intact octopus, and with no visible impact on the behaviour of the animal. This provides exceptional value to these findings for future studies.Summary statementHere we report events occurring after interruption of the peripheral neural circuitry inOctopus vulgaris, from the dramatic loss of normal functioning to full recovery.

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