Immunomodulatory role of short neuropeptide F in the mud crab Scylla paramamosain

2021 ◽  
pp. 104260
Author(s):  
Zhanning Xu ◽  
Yujie Wei ◽  
Huiyang Huang ◽  
Songlin Guo ◽  
Haihui Ye
Keyword(s):  
Scylla Paramamosain ◽  
Mud Crab ◽  
Neuropeptide F

scholarly journals A Possible Role of Allatostatin C in Inhibiting Ecdysone Biosynthesis Revealed in the Mud Crab Scylla paramamosain

2021 ◽  
Vol 8 ◽  
Author(s):  
An Liu ◽  
Wenyuan Shi ◽  
Dongdong Lin ◽  
Haihui Ye
Keyword(s):  
Scylla Paramamosain ◽  
Dependent Manner ◽  
Mud Crab ◽  
Methyl Farnesoate ◽  
Independent Manner ◽  
Wide Range ◽  
Negative Effect

C-type allatostatins (C-type ASTs) are a family of structurally related neuropeptides found in a wide range of insects and crustaceans. To date, the C-type allatostatin receptor in crustaceans has not been deorphaned, and little is known about its physiological functions. In this study, we aimed to functionally define a C-type ASTs receptor in the mud crab, Scylla paramamosian. We showed that C-type ASTs receptor can be activated by ScypaAST-C peptide in a dose-independent manner and by ScypaAST-CCC peptide in a dose-dependent manner with an IC50 value of 6.683 nM. Subsequently, in vivo and in vitro experiments were performed to investigate the potential roles of ScypaAST-C and ScypaAST-CCC peptides in the regulation of ecdysone (20E) and methyl farnesoate (MF) biosynthesis. The results indicated that ScypaAST-C inhibited biosynthesis of 20E in the Y-organ, whereas ScypaAST-CCC had no effect on the production of 20E. In addition, qRT-PCR showed that both ScypaAST-C and ScypaAST-CCC significantly decreased the level of expression of the MF biosynthetic enzyme gene in the mandibular organ, suggesting that the two neuropeptides have a negative effect on the MF biosynthesis in mandibular organs. In conclusion, this study provided new insight into the physiological roles of AST-C in inhibiting ecdysone biosynthesis. Furthermore, it was revealed that AST-C family peptides might inhibit MF biosynthesis in crustaceans.

scholarly journals Role of Oxytocin/Vasopressin-Like Peptide and Its Receptor in Vitellogenesis of Mud Crab

2020 ◽  
Vol 21 (7) ◽  
pp. 2297
Author(s):  
Dongdong Lin ◽  
Yujie Wei ◽  
Haihui Ye
Keyword(s):  
In Situ Hybridisation ◽  
Cerebral Ganglion ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Signaling System ◽  
Regulation Of Metabolism ◽  
F Cells ◽  
17Β Estradiol

Oxytocin (OT)/vasopressin (VP) signaling system is important to the regulation of metabolism, osmoregulation, social behaviours, learning, and memory, while the regulatory mechanism on ovarian development is still unclear in invertebrates. In this study, Spot/vp-like and its receptor (Spot/vpr-like) were identified in the mud crab Scylla paramamosain. Spot/vp-like transcripts were mainly expressed in the nervous tissues, midgut, gill, hepatopancreas, and ovary, while Spot/vpr-like were widespread in various tissues including the hepatopancreas, ovary, and hemocytes. In situ hybridisation revealed that Spot/vp-like mRNA was mainly detected in 6–9th clusters in the cerebral ganglion, and oocytes and follicular cells in the ovary, while Spot/vpr-like was found to localise in F-cells in the hepatopancreas and oocytes in the ovary. In vitro experiment showed that the mRNA expression level of Spvg in the hepatopancreas, Spvgr in the ovary, and 17β-estradiol (E2) content in culture medium were significantly declined with the administration of synthetic SpOT/VP-like peptide. Besides, after the injection of SpOT/VP-like peptide, it led to the significantly reduced expression of Spvg in the hepatopancreas and subduced E2 content in the haemolymph in the crabs. In brief, SpOT/VP signaling system might inhibit vitellogenesis through neuroendocrine and autocrine/paracrine modes, which may be realised by inhibiting the release of E2.

The role of Mu-type glutathione S-transferase in the mud crab (Scylla paramamosain) during ammonia stress

2020 ◽  
Vol 227 ◽  
pp. 108642 ◽  
Author(s):  
Chang-Hong Cheng ◽  
Hong-Ling Ma ◽  
Yi-Qin Deng ◽  
Juan Feng ◽  
Xiao-Long Chen ◽  
...  
Keyword(s):  
Scylla Paramamosain ◽  
Mud Crab ◽  
Glutathione S Transferase ◽  
Ammonia Stress

Short neuropeptide F enhances the immune response in the hepatopancreas of mud crab (Scylla paramamosain)

2020 ◽  
Vol 101 ◽  
pp. 244-251 ◽  
Author(s):  
Zhanning Xu ◽  
Yujie Wei ◽  
Songlin Guo ◽  
Dongdong Lin ◽  
Haihui Ye
Keyword(s):  
Immune Response ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Neuropeptide F

Immunomodulatory role of crustacean cardioactive peptide in the mud crab Scylla paramamosain

2022 ◽  
Author(s):  
Yujie Wei ◽  
Zhanning Xu ◽  
Shuang Hao ◽  
Songlin Guo ◽  
Huiyang Huang ◽  
...  
Keyword(s):  
Scylla Paramamosain ◽  
Mud Crab ◽  
Crustacean Cardioactive Peptide

scholarly journals Chemosensitivity and role of swimming legs of mud crab, Scylla paramamosain, in feeding activity as determined by electrocardiographic and behavioural observations

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11248
Author(s):  
Gunzo Kawamura ◽  
Chi Keong Loke ◽  
Leong Seng Lim ◽  
Annita Seok Kian Yong ◽  
Saleem Mustafa
Keyword(s):  
Scientific Evidence ◽  
Feeding Activity ◽  
Structural Features ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Food Items ◽  
Feeding Activities ◽  
Underlying Mechanisms ◽  
Touch Stimulus

Swimming crabs have a characteristic fifth pair of legs that are flattened into paddles for swimming purposes. The dactyl of these legs bears a thick seta along its edge. The chemoreceptive and feeding properties of the seta are supported with scientific evidence; however, there is no available data on the sensitivity of the setae in portunid crabs. The underlying mechanisms of the chemo- and mechano-sensitivity of appendages and their involvement in feeding activities of the mud crab (Scylla paramamosain) were investigated using electrocardiography and behavioural assay, which focused on the responses of the mud crab to chemical and touch stimulus. Electrocardiography revealed the sensory properties of the appendages. The dactyls of swimming legs and the antennules were chemosensitive, but not mechanosensitive and vice versa for the antennae. However, the mouthparts, claws, and walking legs were chemo- and mechanosensitive. Only the chemosensitive appendages, including the swimming legs, were directly involved in feeding. The flattened dactyls of the swimming legs were more efficient than the pointed dactyls of the walking legs in detecting the food organism crawling on the substrate. The structural features enhanced the capacity of the crab in coming into contact with scattered food items. This study revealed that the swimming legs are important appendages for feeding in the mud crab.

Role of neuroparsin 1 in vitellogenesis in the mud crab, Scylla paramamosain

2020 ◽  
Vol 285 ◽  
pp. 113248 ◽  
Author(s):  
Jing Liu ◽  
An Liu ◽  
Fang Liu ◽  
Huiyang Huang ◽  
Haihui Ye
Keyword(s):  
Scylla Paramamosain ◽  
Mud Crab

scholarly journals miR-2 contributes to WSSV infection by targeting Caspase 2 in mud crab (Scylla paramamosain)

2021 ◽  
Author(s):  
Yi Gong ◽  
Jiao Chen ◽  
Yalei Cui ◽  
Sheng-Kang Li
Keyword(s):  
Immune Response ◽  
White Spot Syndrome Virus ◽  
Marine Invertebrates ◽  
Regulatory Function ◽  
Scylla Paramamosain ◽  
Apoptosis Induction ◽  
Mud Crab ◽  
Caspase 2 ◽  
The Relationship

As we known, Caspase 2 is widely studied for its apoptosis regulatory function in mammals. However, despite the fundamental role of apoptosis during the anti-viral immune response, the relationship between Caspase 2 and virus infection has not been extensively explored in invertebrates, whether miRNAs are involved in this process also remains unclear. To address this issue, the miRNA-mediated regulation of Caspase 2 in mud crab Scylla paramamosain was characterized in this study. The results suggested that Sp-Caspase 2 could suppress white spot syndrome virus (WSSV) infection via apoptosis induction. The further data showed that Caspase 2 was directly targeted by miR-2 in mud crab. Silencing or overexpression of miR-2 could affect apoptosis and WSSV replication through regulating the expression level of Caspase 2. Taken together, all these results demonstrated the crucial role of miR-2-Caspase 2 pathway in the innate immunity of mud crab and revealed a novel mechanism during anti-viral immune response in marine invertebrates.

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