Multilocus sequence typing and repetitive element-based polymerase chain reaction analysis of Neisseria meningitidis isolates in Brazil reveal the emergence of 11 new sequence types genetically related to the ST-32 and ST-41/44 complexes and high prevalence of strains related to hypervirulent lineages

2005 ◽  
Vol 53 (3) ◽  
pp. 161-167 ◽  
Author(s):  
Ivano de Filippis ◽  
Ana Carolina Paulo Vicente
Keyword(s):  
Polymerase Chain Reaction ◽  
Neisseria Meningitidis ◽  
Multilocus Sequence Typing ◽  
Repetitive Element ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
High Prevalence ◽  
Polymerase Chain ◽  
Sequence Types

scholarly journals Occurrence of Staphylococcus aureus in cattle, sheep, goat, and pig rearing in the Czech Republic

2017 ◽  
Vol 86 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Marcela Klimešová ◽  
Ivan Manga ◽  
Ludmila Nejeschlebová ◽  
Jiří Horáček ◽  
Antonín Ponížil ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Repetitive Element ◽  
Polymerase Chain Reaction Analysis ◽  
Polymerase Chain Reaction Method ◽  
Sequence Type ◽  
Chain Reaction ◽  
Goat Farm ◽  
Polymerase Chain ◽  
Cattle Farms

The study focused on the prevalence ofStaphylococcus aureusand resistant strains in livestock. In this study, 114 different samples from three cattle farms (84 from two farms of dairy cows and 30 from one farm of suckler cows), 132 samples from one sheep farm, 120 samples from one goat farm, and 82 samples from three pig farms were examined. Strains identified asStaphylococcus aureuswere further analysed by the polymerase chain reaction method for detection of themecA gene and for confirmation of the sequence type 398. Positive incidence ofStaphylococcus aureuswas confirmed in farms of suckler cows, sheep, goats and pigs. The incidence of methicilin-resistantStaphylococcus aureuswas confirmed at a goat farm, with all strains belonging to the sequence type 398. Repetitive element palindromic-polymerase chain reaction analysis was performed to compare the relatedness of selected human and animalS. aureusstrains at the goat and sheep farms. The obtained data from repetitive element-polymerase chain reaction analysis showed significant clonal similarity among the tested isolates and indicated the possibility of mutual transmission between animals or animal and human and possible transfer in the food chain.

scholarly journals EXPRESS: Clinical performance of the Elecsys® Anti-SARS-CoV-2 combined in an algorithm with two specific anti-IgG immunoassays for the evaluation of the serological response of patients with COVID-19 in a population with a high prevalence of infection

2021 ◽  
pp. 000456322110380
Author(s):  
Xavier Gabaldó-Barrios ◽  
Simona Iftimie ◽  
Anna Hernández-Aguilera ◽  
Isabel Pujol ◽  
Frederic Ballester ◽  
...  
Keyword(s):  
Immune Response ◽  
Polymerase Chain Reaction ◽  
Predictive Value ◽  
Clinical Performance ◽  
Polymerase Chain Reaction Test ◽  
Serological Response ◽  
Chain Reaction ◽  
Automated Assay ◽  
High Prevalence ◽  
Polymerase Chain

Background: Anti-SARS-CoV-2 antibodies have been used in the study of the immune response in infected patients. However, differences in sensitivity and specificity have been reported, depending on the method of analysis. The aim of the present study was to evaluate the diagnostic accuracy of an algorithm in which a high-throughput automated assay for total antibodies was used for screening and two semi-automated IgG-specific methods were used to confirm the results, and also to correlate the analytical results with the clinical data and the time elapsed since infection. Methods: We studied 306 patients, some hospitalized and some outpatients, belonging to a population with a high prevalence of COVID-19. One-hundred and ten patients were classified as SARS-CoV-2 negative and 196 as positive by polymerase chain reaction. Results: The algorithm and automated assay alone had a specificity and a positive predictive value of 100%, although the sensitivity and negative predictive value of the algorithm was higher. Both methods showed a good sensitivity from day 11 of the onset of symptoms in asymptomatic and symptomatic patients. The absorbance of the total antibodies was significantly higher in severely symptomatic than in asymptomatic or mildly symptomatic patients, which suggests the antibody level was higher. We found 15 patients that did not present seroconversion at 12 days from the onset of symptoms or the first polymerase chain reaction test. Conclusion: This study highlights the proper functioning of algorithms in the diagnosis of the immune response to COVID-19, which can help to define testing strategies against this disease.

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