Occurrence of Staphylococcus aureus in cattle, sheep, goat, and pig rearing in the Czech Republic

The study focused on the prevalence ofStaphylococcus aureusand resistant strains in livestock. In this study, 114 different samples from three cattle farms (84 from two farms of dairy cows and 30 from one farm of suckler cows), 132 samples from one sheep farm, 120 samples from one goat farm, and 82 samples from three pig farms were examined. Strains identified asStaphylococcus aureuswere further analysed by the polymerase chain reaction method for detection of themecA gene and for confirmation of the sequence type 398. Positive incidence ofStaphylococcus aureuswas confirmed in farms of suckler cows, sheep, goats and pigs. The incidence of methicilin-resistantStaphylococcus aureuswas confirmed at a goat farm, with all strains belonging to the sequence type 398. Repetitive element palindromic-polymerase chain reaction analysis was performed to compare the relatedness of selected human and animalS. aureusstrains at the goat and sheep farms. The obtained data from repetitive element-polymerase chain reaction analysis showed significant clonal similarity among the tested isolates and indicated the possibility of mutual transmission between animals or animal and human and possible transfer in the food chain.

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Molecular typing of Lactobacillus brevis isolates from Korean food using repetitive element-polymerase chain reaction

Food Science and Technology International ◽

10.1177/1082013217753993 ◽

2018 ◽

Vol 24 (4) ◽

pp. 341-350 ◽

Cited By ~ 1

Author(s):

Jasmine Kaur ◽

Anshul Sharma ◽

Sulhee Lee ◽

Young-Seo Park

Keyword(s):

Polymerase Chain Reaction ◽

Repetitive Element ◽

Bacterial Species ◽

Large Family ◽

Lactobacillus Brevis ◽

Polymerase Chain Reaction Method ◽

Fermented Foods ◽

Chain Reaction ◽

Easy Method ◽

Polymerase Chain

Lactobacillus brevis is a part of a large family of lactic acid bacteria that are present in cheese, sauerkraut, sourdough, silage, cow manure, feces, and the intestinal tract of humans and rats. It finds its use in food fermentation, and so is considered a “generally regarded as safe” organism. L. brevis strains are extensively used as probiotics and hence, there is a need for identifying and characterizing these strains. For identification and discrimination of the bacterial species at the subspecific level, repetitive element-polymerase chain reaction method is a reliable genomic fingerprinting tool. The objective of the present study was to characterize 13 strains of L. brevis isolated from various fermented foods using repetitive element-polymerase chain reaction. Repetitive element-polymerase chain reaction was performed using three primer sets, REP, Enterobacterial Repetitive Intergenic Consensus (ERIC), and (GTG)5, which produced different fingerprinting patterns that enable us to distinguish between the closely related strains. Fingerprinting patterns generated band range in between 150 and 5000 bp with REP, 200–7500 bp with ERIC, and 250–2000 bp with (GTG)5 primers, respectively. The Jaccard’s dissimilarity matrices were used to obtain dendrograms by the unweighted neighbor-joining method using genetic dissimilarities based on repetitive element-polymerase chain reaction fingerprinting data. Repetitive element-polymerase chain reaction proved to be a rapid and easy method that can produce reliable results in L. brevis species.

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