Detection of 10 medically important Candida species by seminested polymerase chain reaction

2012 ◽  
Vol 72 (2) ◽  
pp. 196-198 ◽  
Author(s):  
Leslie Thian Lung Than ◽  
Pei Pei Chong ◽  
Kee Peng Ng ◽  
Heng Fong Seow
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Species ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Seminested Polymerase Chain Reaction

scholarly journals Molecular characterization of Candida dubliniensis and Candida albicans in the oral cavity of drug abusers using duplex polymerase chain reaction

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Parastoo Hassani Abharian ◽  
Parvin Dehghan ◽  
Peyman Hassani Abharian ◽  
Sepideh Tolouei
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Oral Cavity ◽  
Candida Species ◽  
Species Complex ◽  
Candida Dubliniensis ◽  
Drug Abusers ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Duplex Polymerase Chain Reaction

  Background and Purpose: Candida dubliniensis is closely related to the most pathogenic and prevalent yeast, namely C. albicans. Candida species can opportunistically overgrow in vulnerable individuals and cause a variety of diseases. The current study aimed to identify and isolate C. dubliniensis species present in the Candida albicans species complex identified in the oral cavity of drug abusers. Materials and Methods: This study was conducted on 53 strains of C. albicans species complex, isolated from the oral mucosa of drug abusers in Isfahan, Iran. DNA extraction was accomplished through boiling procedure. Duplex polymerase chain reaction (PCR) was performed to amplify ITS1-5.8S-ITS2 region using four specific primers. Fungal species were identified based on the difference in the size of the bands created in the Agarose gel. Results: Out of the 53 isolates under study, 30 (56.6%) and 14 (26.4%) samples were identified as C. albicans and C. dubliniensis, respectively. In the remaining 9 samples (17%), both types of Candida species were confirmed. Conclusion: The findings of the present study revealed the presence of a noticeable amount of C. dubliniensis in the oral cavity of drug abusers. Therefore, the probable presence of this fungus should be considered during the examination of oral infection among this group. To date, no research has directly investigated this issue in Iran.

Determination of candida species from mouth and vagina by use of polymerase chain reaction method

2011 ◽  
Vol 44 (13) ◽  
pp. S130-S131
Author(s):  
Saeid Mahdavi Omran ◽  
Ramazan Rajabnia ◽  
Seyed Ali Asghar Sefidgar ◽  
Seddigheh Esmaeilzadeh ◽  
Maryam Ghasempour ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Species ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

Identification of rare Candida species and other yeasts by polymerase chain reaction and slot blot hybridization

2000 ◽  
Vol 38 (4) ◽  
pp. 207-212 ◽  
Author(s):  
Juergen Loeffler ◽  
Holger Hebart ◽  
Stella Magga ◽  
Diethard Schmidt ◽  
Lena Klingspor ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Species ◽  
Blot Hybridization ◽  
Slot Blot ◽  
Chain Reaction ◽  
Slot Blot Hybridization ◽  
Polymerase Chain
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