ELISA and immuno–polymerase chain reaction assays for the sensitive detection of melioidosis

2013 ◽  
Vol 75 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Alanna Cooper ◽  
Natasha L. Williams ◽  
Jodie L. Morris ◽  
Robert E. Norton ◽  
Natkunam Ketheesan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sensitive Detection ◽  
Chain Reaction ◽  
Polymerase Chain

Sensitive detection and differentiation ofSapporo virus, a member of the familyCaliciviridae, by standard and booster nested polymerase chain reaction

2001 ◽  
Vol 65 (2) ◽  
pp. 413-417 ◽  
Author(s):  
Shinjiro Honma ◽  
Shuji Nakata ◽  
Yoshiyuki Sakai ◽  
Masatoshi Tatsumi ◽  
Kazuko Numata-Kinoshita ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sensitive Detection ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Noncompetitive Phage Anti-Immunocomplex Real-Time Polymerase Chain Reaction for Sensitive Detection of Small Molecules

2011 ◽  
Vol 83 (1) ◽  
pp. 246-253 ◽  
Author(s):  
Hee-Joo Kim ◽  
Mark McCoy ◽  
Shirley J. Gee ◽  
Gualberto G. González-Sapienza ◽  
Bruce D. Hammock
Keyword(s):  
Polymerase Chain Reaction ◽  
Small Molecules ◽  
Real Time ◽  
Sensitive Detection ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Use of Touch-Down Polymerase Chain Reaction to Enhance the Sensitivity of Mycobacterium Bovis Detection

2005 ◽  
Vol 17 (3) ◽  
pp. 232-238 ◽  
Author(s):  
Martín J. Zumárraga ◽  
Virginia Meikle ◽  
Amelia Bernardelli ◽  
Alejandro Abdala ◽  
Hector Tarabla ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Bovis ◽  
Pcr Amplification ◽  
Sensitive Detection ◽  
Chain Reaction ◽  
Single Tube ◽  
Nasal Swabs ◽  
Diagnostic Samples ◽  
Polymerase Chain ◽  
Duplication Time

The confirmatory diagnosis of Mycobacterium bovis ( M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS 6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis–positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification.

Sign in / Sign up

Export Citation Format

Share Document