A highly sensitive fluorescent acidic pH probe based on rhodamine B diethyl-2-aminobutenedioate conjugate and its application in living cells

2013 ◽  
Vol 96 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Zhi-Qiang Hu ◽  
Ming Li ◽  
Ming-Dong Liu ◽  
Wen-Ming Zhuang ◽  
Guang-Ke Li
Keyword(s):  
Rhodamine B ◽  
Living Cells ◽  
Acidic Ph ◽  
Ph Probe ◽  
Highly Sensitive

A rhodamine B-based lysosomal pH probe

2015 ◽  
Vol 3 (5) ◽  
pp. 919-925 ◽  
Author(s):  
Shi-Li Shen ◽  
Xin-Peng Chen ◽  
Xiao-Fan Zhang ◽  
Jun-Ying Miao ◽  
Bao-Xiang Zhao
Keyword(s):  
Response Time ◽  
Rhodamine B ◽  
High Selectivity ◽  
High Sensitivity ◽  
Living Cells ◽  
Acidic Ph ◽  
Ph Change ◽  
Short Response Time ◽  
Lysosomal Ph ◽  
Ph Probe

A novel rhodamine B-based lysosomal pH probe RML was developed. RML responded to acidic pH with short response time, high selectivity and high sensitivity and could detect lysosomal pH change in living cells.

A Highly Sensitive Quinoline-Containing Rhodamine B Thiohydrazide Based Fluorescent Probe for Hg2+in Aqueous Solution and Living Cells

2010 ◽  
Vol 43 (17) ◽  
pp. 2751-2761 ◽  
Author(s):  
Zhi-Xiang Han ◽  
Xiao-Bing Zhang ◽  
Zhuo Li ◽  
Guo-Jiang Mao ◽  
Zhen Jin ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Fluorescent Probe ◽  
Rhodamine B ◽  
Living Cells ◽  
Highly Sensitive

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

Synthesis and 31 P NMR characterization of new low toxic highly sensitive pH probes designed for in vivo acidic pH studies

2002 ◽  
Vol 10 (5) ◽  
pp. 1451-1458 ◽  
Author(s):  
Sophie Martel ◽  
Jean-Louis Clément ◽  
Agnès Muller ◽  
Marcel Culcasi ◽  
Sylvia Pietri
Keyword(s):  
Acidic Ph ◽  
Highly Sensitive ◽  
Nmr Characterization ◽  
Low Toxic ◽  
Ph Probes

A highly-sensitive “turn on” probe based on coumarin β-diketone for hydrazine detection in PBS and living cells

2021 ◽  
Vol 252 ◽  
pp. 119510
Author(s):  
Rui Chen ◽  
Guang-Jin Shi ◽  
Jia-Jia Wang ◽  
Hai-Feng Qin ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Living Cells ◽  
Turn On ◽  
Highly Sensitive

Highly sensitive real-time detection of intracellular oxidative stress and application in mycotoxin toxicity evaluation based on living single-cell electrochemical sensors

The Analyst ◽  
2021 ◽  
Author(s):  
Lu Gao ◽  
Jiadi Sun ◽  
Liping Wang ◽  
Qigao Fan ◽  
Gaowen Zhu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Electrochemical Sensor ◽  
Single Cell ◽  
Real Time ◽  
Electrochemical Sensors ◽  
Living Cells ◽  
Selective Detection ◽  
Toxicity Evaluation ◽  
Highly Sensitive ◽  
Intracellular Oxidative Stress

Single-cell electrochemical sensor is used in the local selective detection of living cells because of its high spatial–temporal resolution and sensitivity, as well as its ability to obtain comprehensive cellular physiological states and processes.

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