Photophysico-chemical properties and photoinactivation of Staphylococcus Aureus using zinc phthalocyanines linked silver nanoparticles conjugates

2020 ◽  
Vol 176 ◽  
pp. 108237 ◽  
Author(s):  
Gauta Gold Matlou ◽  
Tebello Nyokong
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Chemical Properties ◽  
Zinc Phthalocyanines

Antibacterial and Cytotoxic Effects of Silver Nanoparticles on Staphylococcus aureus and Normal Vero Cells

2019 ◽  
Vol 22 (10) ◽  
pp. 184-190
Author(s):  
Rasha Hadi Saleh ◽  
Entisar J. Al-Mukhtar ◽  
Zaytoon A. Al-Khafaji ◽  
Mohammed H. Al Hasnawy ◽  
Huda H. Al-Hasnawy
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Vero Cells ◽  
Cytotoxic Effects

scholarly journals Silver Nanoparticles Stabilized with Phosphorus-Containing Heterocyclic Surfactants: Synthesis, Physico-Chemical Properties, and Biological Activity Determination

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1883
Author(s):  
Martin Pisárčik ◽  
Miloš Lukáč ◽  
Josef Jampílek ◽  
František Bilka ◽  
Andrea Bilková ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biological Activity ◽  
Zeta Potential ◽  
Chain Length ◽  
Alkyl Chain ◽  
Chemical Properties ◽  
Alkyl Chain Length ◽  
Microbial Pathogens ◽  
Physico Chemical ◽  
Phosphorus Containing

Phosphorus-containing heterocyclic cationic surfactants alkyldimethylphenylphospholium bromides with the alkyl chain length 14 to 18 carbon atoms were used for the stabilization of silver nanodispersions. Zeta potential of silver nanodispersions ranges from +35 to +70 mV, which indicates the formation of stable silver nanoparticles (AgNPs). Long-chain heptadecyl and octadecyl homologs of the surfactants series provided the most intensive stabilizing effect to AgNPs, resulting in high positive zeta potential values and smaller diameter of AgNPs in the range 50–60 nm. A comparison with non-heterocyclic alkyltrimethylphosphonium surfactants of the same alkyl chain length showed better stability and more positive zeta potential values for silver nanodispersions stabilized with heterocyclic phospholium surfactants. Investigations of biological activity of phospholium-capped AgNPs are represented by the studies of antimicrobial activity and cytotoxicity. While cytotoxicity results revealed an increased level of HepG2 cell growth inhibition as compared with the cytotoxicity level of silver-free surfactant solutions, no enhanced antimicrobial action of phospholium-capped AgNPs against microbial pathogens was observed. The comparison of cytotoxicity of AgNPs stabilized with various non-heterocyclic ammonium and phosphonium surfactants shows that AgNPs capped with heterocyclic alkyldimethylphenylphospholium and non-heterocyclic triphenyl-substituted phosphonium surfactants have the highest cytotoxicity among silver nanodispersions stabilized by the series of ammonium and phosphonium surfactants.

scholarly journals The Antimicrobial and Anti-Inflammatory Effects of Silver Nanoparticles Synthesised from Cotyledon orbiculata Aqueous Extract

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1343
Author(s):  
Caroline Tyavambiza ◽  
Abdulrahman Mohammed Elbagory ◽  
Abram Madimabe Madiehe ◽  
Mervin Meyer ◽  
Samantha Meyer
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Methicillin Resistance ◽  
Antimicrobial Activities ◽  
Transmission Electron ◽  
Anti Inflammatory ◽  
Anti Inflammation ◽  
Important Medicinal Plant ◽  
Pro Inflammatory Cytokines

Cotyledon orbiculata, commonly known as pig’s ear, is an important medicinal plant of South Africa. It is used in traditional medicine to treat many ailments, including skin eruptions, abscesses, inflammation, boils and acne. Many plants have been used to synthesize metallic nanoparticles, particularly silver nanoparticles (AgNPs). However, the synthesis of AgNPs from C. orbiculata has never been reported before. The aim of this study was to synthesize AgNPs using C. orbiculata and evaluate their antimicrobial and immunomodulatory properties. AgNPs were synthesized and characterized using Ultraviolet-Visible Spectroscopy (UV-Vis), Dynamic Light Scattering (DLS) and High-Resolution Transmission Electron Microscopy (HR-TEM). The antimicrobial activities of the nanoparticles against skin pathogens (Staphylococcus aureus, Staphylococcus epidermidis, Methicillin Resistance Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans) as well as their effects on cytokine production in macrophages (differentiated from THP-1 cells) were evaluated. The AgNPs from C. orbiculata exhibited antimicrobial activity, with the highest activity observed against P. aeruginosa (5 µg/mL). The AgNPs also showed anti-inflammatory activity by inhibiting the secretion of pro-inflammatory cytokines (TNF-alpha, IL-6 and IL-1 beta) in lipopolysaccharide-treated macrophages. This concludes that the AgNPs produced from C. orbiculata possess antimicrobial and anti-inflammation properties.

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

2013 ◽  
Vol 14 (1-2) ◽  
pp. 49-60 ◽  
Author(s):  
Francesca Broggi ◽  
Jessica Ponti ◽  
Guido Giudetti ◽  
Fabio Franchini ◽  
Vicki Stone ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Transformation ◽  
Micronucleus Test ◽  
Chemical Properties ◽  
Neoplastic Transformation ◽  
Research Centre ◽  
Ag Nps ◽  
Joint Research ◽  
Ic50 Values ◽  
Average Size

AbstractSilver nanoparticles (Ag NPs) are one of the most common nanomaterials present in nanotechnology-based products. Here, the physical chemical properties of Ag NPs suspensions of 44 nm, 84 nm and 100 nm sizes synthesized in our laboratory were characterized. The NM-300 material (average size of 17 nm), supplied by the Joint Research Centre Nanomaterials Repository was also included in the present study. The Ag NPs potential cytotoxicity was tested on the Balb3T3 cell line by the Colony Forming Efficiency assay, while their potential morphological neoplastic transformation and genotoxicity were tested by the Cell Transformation Assay and the micronucleus test, respectively. After 24 h of exposure, NM-300 showed cytotoxicity with an IC50 of 8 µM (corresponding to 0.88 µg/mL) while for the other nanomaterials tested, values of IC50 were higher than 10 µM (1.10 µg/mL). After 72 h of exposure, Ag NPs showed size-dependent cytotoxic effect with IC50 values of 1.5 µM (1.16 µg/mL) for NM-300, 1.7 µM (1.19 µg/mL) for Ag 44 nm, 1.9 µM (0.21 µg/mL) for Ag 84 nm and 3.2 µM (0.35 µg/mL) for Ag 100 nm. None of the Ag NPs tested was able to induce either morphological neoplastic transformation or micronuclei formation.

scholarly journals Optical characterization and concentration-dependent antibacterial activity of silver nanoparticles (AgNPs) using aqueous Melastoma malabathricum extract against Staphylococcus aureus

2020 ◽  
Vol 11 (4) ◽  
pp. 5382-5387
Author(s):  
Irshad Ul Haq Bhat ◽  
Maisarah Binti Alias
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Functional Groups ◽  
Diffusion Method ◽  
Synthetic Approach ◽  
Potential Candidate ◽  
X Ray ◽  
Melastoma Malabathricum ◽  
Resonance Band

The approach towards green synthetic methods has been enormously encouraged to synthesise nanoparticles for various uses. In this study, the one-pot synthetic method was adapted to synthesise silver nanoparticles (AgNPs) using Melastoma malabathricum (M. malabathricum) aqueous extract. The formation of AgNPs was confirmed by observing the results obtained by optical characterisation methods. The plasma resonance band along with shoulder at 375 nm and 595 nm, respectively, in Uv-Visible spectra supported the conversion of silver (Ag) to AgNPs reduced by functional groups present in the plant extract. The size of AgNPs was 31 nm and cubic in shape as confirmed by X-ray diffractometry (XRD) using Scherer equation. X-Ray Fluorescence (XRF) results also confirmed the presence of silver. The FTIR characterisation confirmed the presence of reducing functional groups. The antibacterial activity of AgNPs against Staphylococcus aureus (S. aureus) was carried out by disc diffusion method with increasing concentration of AgNPs, and enhanced inhibition zone was observed. The AgNPs obtained can be further explored against different bacterial strains and can a potential candidate as an antibacterial agent using the green synthetic approach.

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