scholarly journals Optical characterization and concentration-dependent antibacterial activity of silver nanoparticles (AgNPs) using aqueous Melastoma malabathricum extract against Staphylococcus aureus

2020 ◽  
Vol 11 (4) ◽  
pp. 5382-5387
Author(s):  
Irshad Ul Haq Bhat ◽  
Maisarah Binti Alias
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Functional Groups ◽  
Diffusion Method ◽  
Synthetic Approach ◽  
Potential Candidate ◽  
X Ray ◽  
Melastoma Malabathricum ◽  
Resonance Band

The approach towards green synthetic methods has been enormously encouraged to synthesise nanoparticles for various uses. In this study, the one-pot synthetic method was adapted to synthesise silver nanoparticles (AgNPs) using Melastoma malabathricum (M. malabathricum) aqueous extract. The formation of AgNPs was confirmed by observing the results obtained by optical characterisation methods. The plasma resonance band along with shoulder at 375 nm and 595 nm, respectively, in Uv-Visible spectra supported the conversion of silver (Ag) to AgNPs reduced by functional groups present in the plant extract. The size of AgNPs was 31 nm and cubic in shape as confirmed by X-ray diffractometry (XRD) using Scherer equation. X-Ray Fluorescence (XRF) results also confirmed the presence of silver. The FTIR characterisation confirmed the presence of reducing functional groups. The antibacterial activity of AgNPs against Staphylococcus aureus (S. aureus) was carried out by disc diffusion method with increasing concentration of AgNPs, and enhanced inhibition zone was observed. The AgNPs obtained can be further explored against different bacterial strains and can a potential candidate as an antibacterial agent using the green synthetic approach.

scholarly journals Green Synthesis of Silver Nanoparticles Using Moringa Oleifera Leaf Extract, Tamarindus Indica Fruit Extract and Assessement of Antibacterial Activity Against Staphylococcus Aureus

2021 ◽  
Author(s):  
Shirisha A ◽  
ANUMOLU VIJAYA KUMAR ◽  
Laxman Chatlod R ◽  
Shashi Kumar M ◽  
Krishnaiah N ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Diffusion Method ◽  
Tamarindus Indica ◽  
Fruit Extract

Abstract The present study mainly deals with the green synthesis, characterization and evaluation of antibacterial properties of silver nanoparticles (AgNPs) synthesized by using the leaf extract of Moringa oleifera and fruit extract of Tamarindus indica. In this study for synthesis of silver nanoparticles different ratios of 1mM silver nitrate and Moringa oleifera leaf extract i.e, 95:5, 90:10 and 85: 15 was taken in conical flask and kept for one 1 hr at 25 0 c on magnetic stirrer, out of which 90:10 ratio was selected for further study based on highest peak, good size and stability. Tamarindus indica fruit extract was added to silver nitrate solution till the colour of the solution changes from light brown to chocolate brownish colour. The synthesized silver nanoparticles were characterized by UV-Visible spectroscopy, Zeta potential, size distribution by intensity. The absorption spectrum of the silver nano solution prepared by using Moringa oleifera and Tamarindus indica fruit extract showed a surface plasmon absorption band with maximum of 420 nm and 430 nm respectively indicating the presence of silver nanoparticles. The zeta value of silver nanoparticles synthesized from Moringa oleifera and Tamarindus indica fruit extract was -12.5 mV and -15.5 mV, size of 110.2 nm and 130.2 nm respectively. The antibacterial efficacy of nanosilver was checked by agar well diffusion method, and the silver nanoparticles showed effective antibacterial activity against Staphylococcus aureus.

scholarly journals Microwave Assisted Green Synthesis of Silver Nanoparticles using Pomegranate Peel Extract: Characterization and Antibacterial Activity Studies

2019 ◽  
Vol 32 (2) ◽  
pp. 458-462
Author(s):  
Gugulothu Yaku ◽  
Bandi Rajkumar ◽  
T.V.D. Prasad Rao
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Diffusion Method ◽  
Microwave Assisted Synthesis ◽  
Pomegranate Peel ◽  
Stabilizing Agent ◽  
X Ray ◽  
Microwave Assisted ◽  
Pomegranate Peel Extract

In the present work, a simple and low cost and eco-friendly technique is applied for the microwave assisted synthesis of silver nanoparticles (AgNPs) using the extract of pomegranate peel wastes, which does not require any use of external stabilizing agent. The extract of pomegranates peelings waste served as a reducing as well as capping/stabilizing agent. The synthesized silver nanoparticles were characterized by using powder X-ray diffraction (XRD), transmission eelectron microscopy (TEM), FT-IR, UV-visible spectroscopy and scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX). The stability of AgNPs was analyzed by zeta potential measurements. The antibacterial activity of synthesized AgNPs was also evaluated on six Gram-positive and Gram-negative bacteria using agar well diffusion method.

scholarly journals Antimicrobial Potential of Biosynthesized Silver Nanoparticles by Aaronsohnia factorovskyi Extract

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 130
Author(s):  
Fatimah Al-Otibi ◽  
Reem A. Al-Ahaidib ◽  
Raedah I. Alharbi ◽  
Rana M. Al-Otaibi ◽  
Gadah Albasher
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Plant Extracts ◽  
Fusarium Solani ◽  
Large Scale ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method

The green biosynthesis of nanoparticles by plant extracts is an attractive and promising technique for medicinal applications. In the current study, we chose one of the daisy plants, Aaronsohnia factorovskyi (which grows in the Najd region, Saudi Arabia), to investigate its anti-microbial efficacy, in combination with silver nanoparticles. The biosynthesized nanoparticles were evaluated for antibacterial activity against Staphylococcus aureus, Bacillussubtilis (Gram-positive), Pseudomonas aeruginosa, and Escherichia coli, (Gram-negative) using the disc diffusion method, while the antifungal activity was assessed against Fusarium oxysporum, Fusarium solani, Helminthosporiumrostratum, and Alternariaalternata. The potential phytoconstituents of the plant extracts were identified by Fourier-transform infrared spectroscopy (FT-IR) techniques, the Field emission scanning electron microscopy (FE-SEM), Chromatography/Mass Spectrometry (GC-MS) techniques, and Zeta potential analysis. The current study revealed the ability of the tested plant extract to convert silver ions to silver nanoparticles with an average diameter of 104–140 nm. Biogenic Aaronsohnia factorovskyi-silver nanoparticles (AF-AgNPs) showed significant antibacterial activity against Staphylococcus aureus with inhibition zone diameter to 19.00 ± 2.94 mm, and antifungal activity against Fusarium solani, which reduced the growth of fungal yarn to 1.5 mm. The innovation of the present study is that the green synthesis of NPs, which is simple, cost-effective, provides stable nano-materials, and can be an alternative for the large-scale synthesis of silver nanoparticles.

scholarly journals GREEN SYNTHESIS, CHARACTERIZATION OF SILVER NANOPARTICLES OF A MARINE RED ALGA SPYRIDIA FUSIFORMIS AND THEIR ANTIBACTERIAL ACTIVITY

2017 ◽  
Vol 9 (5) ◽  
pp. 192 ◽  
Author(s):  
Subbiah Murugesan ◽  
Sundaresan Bhuvaneswari ◽  
Vajiravelu Sivamurugan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Red Alga ◽  
Diffusion Method ◽  
Present System ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Uv Visible

Objective: In the present system, the green synthesis of silver nanoparticles using marine the red alga Spyridia fusiformis and antibacterial activity was carried out.Methods: The seaweed extract was used for the synthesis of AgNPs at room temperature. The silver nanoparticles were characterized by using UV–Visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscope and X-ray diffraction (XRD) techniques. The antibacterial activity of biosynthesized silver nanoparticles was carried out by disc diffusion method against pathogenic bacteria.Results: The UV-visible spectroscopy revealed surface plasmon resonance at 450 nm. The FT-IR measurements showed the possible functional groups responsible for the formation of nanoparticles. The X-ray diffraction analysis showed that the particles were crystalline in nature. TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5–50 nm. The silver nanoparticles synthesized from the S. fusiformis showed higher activity and proved their efficacy in controlling the pathogenic bacterial strains. The nanoparticles showed highest inhibition activity on K. pneumaniae and S. aureus up to 26 and 24±0.01 mm at 100 μg/ml of nanoparticles.Conclusion: The synthesised AgNPs have shown the best antibacterial activity against human pathogens E. coli, K. pneumoniae, S. aureus and P. aeruginosa. The above eco-friendly AgNPs synthesis procedure could be a viable solution for industrial applications in the future and therapeutic needs.

scholarly journals The Use of Salacca Zalacca Extract as Reducing Agent to Synthesize Silver Nanoparticles (Agnps) and the Antibacterial Activities.

2019 ◽  
Vol 35 (5) ◽  
pp. 1557-1564
Author(s):  
Lis Permana Sari ◽  
Zuhdi Saputro ◽  
Maximus Pranjoto Utomo ◽  
Anti Kolonial Prodjosantoso
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Incubation Time ◽  
Salmonella Typhi ◽  
Reducing Agent ◽  
Diffusion Method ◽  
One Pot ◽  
Extract Concentration ◽  
Synthesize Silver Nanoparticles

In general, leaf, flower, and fruit of plants contain organic compounds which may reduce metal salts to nanosize metals, or nanoparticles (NPs). The extract of snake fruit (Salacca zallaca) has been used to synthesize silver nanoparticles (AgNPs) using green-one pot method. Some reaction variables ie. incubation time, Salacca zallacca extract concentration, and pH of the mixture of AgNO3 and the extract have been set-up and controlled. The AgNPs were characterized using UV-Vis, FTIR, XRD, and TEM methods. Testing the antibacterial activity of AgNPs was undertaken using the disk diffusion method by measuring the inhibition zones of Staphylococcus aureus and Salmonella typhi bacteria.The AgNPs can be prepared by reducing Ag+ using Salacca zalacca extract as reducing agent. The formation of AgNPs are affected by the incubation time, extract concentration, and pH of the solution. The AgNPs indicate SPR peak absorption at 400-530 nm, and centered cubic (fcc) structures with a particle size of 10 nm. The AgNPs show antibacterial activity against Staphylococcus aureus and Salmonella typhi bacteria.

scholarly journals Biogenic Synthesis of Silver Nanoparticles (AgNPs) using Celosia cristata L. Leaves Extract and Their Antimicrobial Activity against Otorhinolaryngological Isolated Pathogen

2019 ◽  
Vol 11 (06) ◽  
Author(s):  
Ramveer Singh ◽  
Navneet Kumar
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Nitrate ◽  
Positive Control ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
Disc Diffusion Method ◽  
Biogenic Synthesis ◽  
X Ray ◽  
Celosia Cristata

This work focused on the biogenic synthesis of silver nanoparticles (AgNPs) by silver nitrate using Celosia cristata leaves extract in four different solvents namely petroleum ether, acetone, methanol, and water. Silver nitrate and leaves extract were used as a precursor and capping reducing agent respectively. Biogenic AgNPs were characterized and identified by UV-Vis spectrophotometer, X-ray diffraction (XRD), Field emission scanning electron microscope (FE-SEM), and energy dispersive spectroscopy (EDX). Disc diffusion method was used for antibacterial activity of AgNPs and effective antibacterial activity was shown against IS-3, IS-4, IS-6, and IS-7 as compared to a positive control (ciprofloxacin).

scholarly journals Antibacterial activity and toxicological evaluation of Anogeissusleiocarpus and Psidium guajava on Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 14 (1) ◽  
pp. 214-220
Author(s):  
S.A. Yabo ◽  
S.B. Manga ◽  
A.S. Baki ◽  
R.F. Atata ◽  
S. Gambo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Potential Candidate ◽  
Toxicological Evaluation ◽  
E Coli ◽  
Function Tests ◽  
And Staphylococcus Aureus

The study was carried out to determine the antibacterial activities and toxicological evaluation of Anogeissusleiocarpus and Psidiumguajava on Escherichia coli and Staphylococcus aureus isolated from clinical samples. The plants leaves were extracted using Hexane, Methanol, Ethanol and Water. Various concentrations (50, 25, 12.5 and 6.25mg/ml) of the crude extract of the plants were prepared and their antibacterial activity against Staphylococcus aureus and E. coli was determined using Agar well (Diffusion) method. Toxicity of the plants was evaluated, acute toxicity test, kidney and liver function tests. The result revealed that at 50mg/ml concentration, the leaf extract of Psidiumguajava was active against Staphylococcus aureus and E. coli exhibiting the highest zones of inhibition of 19mm and 9mm respectively. Whereas The leaf extract of Anogeissusleiocarpus only inhibited the growth of Staphylococcus aureus recording highest zone of inhibition of 15mm at a concentration of 50mg/ml. The plant extracts were found to be non-toxic as the LD50  was above 5000mg/kg and the biochemical parameters evaluated for both liver and kidney function tests revealed values that are within normal range. Hence the study established that consumption of the leaves of P.guajava for medicinal purpose can be said to be innocuous, as such the plant could be regarded as a potential candidate in the search of potent and harmless plants of therapeutic value.

scholarly journals Antibacterial Activity Test of the Ethanol in Leaves Extract of Senduduk (Melastoma malabathricum L.) Against Escherichia coli and Staphylococcus aureus

2020 ◽  
Vol 6 (2) ◽  
pp. 139-152
Author(s):  
Alfi Sapitri ◽  
Nofita Lara ◽  
Panal Sitorus
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Wild Plants ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Melastoma Malabathricum ◽  
And Staphylococcus Aureus

Senduduk (Melastoma malabathricum L.) is one of the wild plants used as traditional medicine. Senduduk leaves are used as a medicine for sprue, boils, diarrhea and smallpox. The purpose of this study was to determine the antibacterial activity of the ethanol extract of senduduk leaves of the against Escherichia coli and Staphylococcus aureus. Senduduk leaves extracted by maceration using 96% ethanol solvent. Antibacterial activity testing is carried out using the diffusion method using paper discs.This experiment was repeated three times using concentrations of 20%, 40%, 60% and 80%.Ethanol extract of the senduduk leaves contains flavonoids, saponins, tannins and steroids/triterpenoids. The test results showed that the ethanol extract of the leaves has antibacterial activity against Escherichia coli and Staphylococcus aureus which is characterized by the inhibition zone formed around the disc paper. In Escherichia coli bacteria the smallest inhibition zone at a concentration of 20% with a diameter of inhibition zone 11 mm and the largest diameter of the inhibitory zone at a concentration of 80% with a diameter of inhibition zone 19 mm. whereas for the smallest inhibitory zone Staphylococcus aureus bacteria at a concentration of 20% with an inhibition zone diameter of 12.6 mm and the largest inhibitory zone dameter at a concentration of 80% with a diameter of the inhibitory zone 21,3 mm.The results of the study concluded that the ethanol extract of Senduduk leaves has antibacterial activity against Escherichia coli and Staphylococcus aureus.

scholarly journals Herbal Plant Synthesis of Antibacterial Silver Nanoparticles by Solanum trilobatum and Its Characterization

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
M. Vanaja ◽  
K. Paulkumar ◽  
G. Gnanajobitha ◽  
S. Rajeshkumar ◽  
C. Malarkodi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Electron Microscope ◽  
Green Synthesis ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Solanum Trilobatum ◽  
Green Synthesized Silver Nanoparticles

Green synthesis method of nanomaterials is rapidly growing in the nanotechnology field; it replaces the use of toxic chemicals and time consumption. In this present investigation we report the green synthesis of silver nanoparticles (AgNPs) by using the leaf extract of medicinally valuable plant Solanum trilobatum. The influence of physical and chemical parameters on the silver nanoparticle fabrication such as incubation time, silver nitrate concentration, pH, and temperature is also studied in this present context. The green synthesized silver nanoparticles were characterized by UV-vis spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), energy dispersive X-ray (EDX), and transmission electron microscope (TEM). The SEM and TEM confirm the synthesis of spherical shape of nanocrystalline particles with the size range of 2–10 nm. FTIR reveals that the carboxyl and amine groups may be involved in the reduction of silver ions to silver nanoparticles. Antibacterial activity of synthesized silver nanoparticles was done by agar well diffusion method against different pathogenic bacteria. The green synthesized silver nanoparticles can be used in the field of medicine, due to their high antibacterial activity.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

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