scholarly journals The relationship between culturable doxycycline-resistant bacterial communities and antibiotic resistance gene hosts in pig farm wastewater treatment plants

2020 ◽  
Vol 206 ◽  
pp. 111164
Author(s):  
Yiwen Yang ◽  
Yingxi Chen ◽  
Yingfeng Cai ◽  
Sicheng Xing ◽  
Jiandui Mi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Gene ◽  
Bacterial Communities ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Gene ◽  
Pig Farm ◽  
The Relationship ◽  
Farm Wastewater

scholarly journals Occurrence and Distribution of Antibiotic Resistance Genes in Municipal Wastewater Treatment Plants with D-Type Filters

Water ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 3398
Author(s):  
Haoze Wang ◽  
Bing Li ◽  
Jiaheng Zhao ◽  
Yongjing Tian ◽  
Yong Qiu
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Gene ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Gene ◽  
Municipal Wastewater Treatment ◽  
Secondary Clarifier ◽  
Municipal Wastewater Treatment Plants ◽  
Type Fiber

Filters are popularly used in municipal wastewater treatment plants (WWTPs) as the final guards against effluent solids; however, their impacts on antibiotic resistance gene (ARG) removal in the WWTPs are still unclear. In this study, metagenomic analysis was used to find out the distribution characteristics of ARGs in two WWTPs equipped with the same D-Type fiber filters. Samples of influent, activated sludge liquor, secondary clarifier effluent, and D-Type filter effluent were found to host 695, 609, 675, and 643 ARG subtypes, respectively. The detected ARGs mainly included macB (4.1–8.9%), sav1866 (1.7–3.4%), and oleC (1.6–3.8%). Co-occurrence network analysis combined with contribution analysis helped to identify the ARG-related risks in the samples. Microbacterium, Acinetobacter, Gordonia, and Streptomyces significantly correlated with more than ten kinds of ARG subtypes, implying that they are potential hosts for these resistance gene subtypes. The number of ARG subtypes in the D-Type filter was less than those in the secondary clarifier effluent, indicating the potential of D-Type filters to effectively reduce the ARGs released into the environment. However, the abundance of two pathogens, Mycobacterium and PmrA, increased after the treatment by the D-Type filter, which may reveal the adverse effects of intercepting ARGs inside the fibers. The results may help the understanding of the complex role of the D-Type fiber filter on ARG distribution in WWTPs.

scholarly journals Wastewater pollution differently affects the antibiotic resistance gene pool and biofilm bacterial communities across streambed compartments

2017 ◽  
Vol 26 (20) ◽  
pp. 5567-5581 ◽  
Author(s):  
Jèssica Subirats ◽  
Xavier Triadó-Margarit ◽  
Ladislav Mandaric ◽  
Vicenç Acuña ◽  
José Luis Balcázar ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Bacterial Communities ◽  
Gene Pool ◽  
Antibiotic Resistance Gene ◽  
Wastewater Pollution

scholarly journals A survey of crop-derived transgenes in activated and digester sludges in wastewater treatment plants in the United States

2018 ◽  
Vol 77 (7) ◽  
pp. 1810-1818 ◽  
Author(s):  
Courtney M. Gardner ◽  
Carley A. Gwin ◽  
Claudia K. Gunsch
Keyword(s):  
United States ◽  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Gene ◽  
Antibiotic Resistance Genes ◽  
Transgenic Crops ◽  
The United States ◽  
Transgenic Crop ◽  
Nptii Gene

Abstract The use of transgenic crops has become increasingly common in the United States over the last several decades. Increasing evidence suggests that DNA may be protected from enzymatic digestion and acid hydrolysis in the digestive tract, suggesting that crop-derived transgenes may enter into wastewater treatment plants (WWTPs) intact. Given the historical use of antibiotic resistance genes as selection markers in transgenic crop development, it is important to consider the fate of these transgenes. Herein we detected and quantified crop-derived transgenes in WWTPs. All viable US WWTP samples were found to contain multiple gene targets (p35, nos, bla and nptII) at significantly higher levels than control samples. Control wastewater samples obtained from France, where transgenic crops are not cultivated, contained significantly fewer copies of the nptII gene than US activated and digester sludges. No significant differences were measured for the bla antibiotic resistance gene (ARG). In addition, a nested PCR (polymerase chain reaction) assay was developed that targeted the bla ARG located in regions flanked by the p35 promoter and nos terminator. Overall this work suggests that transgenic crops may have provided an environmental source of nptII; however, follow-up studies are needed to ascertain the viability of these genes as they exit WWTPs.

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