Exploring abundance, diversity and variation of a widespread antibiotic resistance gene in wastewater treatment plants

2018 ◽  
Vol 117 ◽  
pp. 186-195 ◽  
Author(s):  
Ziyan Wei ◽  
Kai Feng ◽  
Shuzhen Li ◽  
Yu Zhang ◽  
Hongrui Chen ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Gene ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Gene

scholarly journals Occurrence and Distribution of Antibiotic Resistance Genes in Municipal Wastewater Treatment Plants with D-Type Filters

Water ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 3398
Author(s):  
Haoze Wang ◽  
Bing Li ◽  
Jiaheng Zhao ◽  
Yongjing Tian ◽  
Yong Qiu
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Gene ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Gene ◽  
Municipal Wastewater Treatment ◽  
Secondary Clarifier ◽  
Municipal Wastewater Treatment Plants ◽  
Type Fiber

Filters are popularly used in municipal wastewater treatment plants (WWTPs) as the final guards against effluent solids; however, their impacts on antibiotic resistance gene (ARG) removal in the WWTPs are still unclear. In this study, metagenomic analysis was used to find out the distribution characteristics of ARGs in two WWTPs equipped with the same D-Type fiber filters. Samples of influent, activated sludge liquor, secondary clarifier effluent, and D-Type filter effluent were found to host 695, 609, 675, and 643 ARG subtypes, respectively. The detected ARGs mainly included macB (4.1–8.9%), sav1866 (1.7–3.4%), and oleC (1.6–3.8%). Co-occurrence network analysis combined with contribution analysis helped to identify the ARG-related risks in the samples. Microbacterium, Acinetobacter, Gordonia, and Streptomyces significantly correlated with more than ten kinds of ARG subtypes, implying that they are potential hosts for these resistance gene subtypes. The number of ARG subtypes in the D-Type filter was less than those in the secondary clarifier effluent, indicating the potential of D-Type filters to effectively reduce the ARGs released into the environment. However, the abundance of two pathogens, Mycobacterium and PmrA, increased after the treatment by the D-Type filter, which may reveal the adverse effects of intercepting ARGs inside the fibers. The results may help the understanding of the complex role of the D-Type fiber filter on ARG distribution in WWTPs.

scholarly journals A survey of crop-derived transgenes in activated and digester sludges in wastewater treatment plants in the United States

2018 ◽  
Vol 77 (7) ◽  
pp. 1810-1818 ◽  
Author(s):  
Courtney M. Gardner ◽  
Carley A. Gwin ◽  
Claudia K. Gunsch
Keyword(s):  
United States ◽  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Gene ◽  
Antibiotic Resistance Genes ◽  
Transgenic Crops ◽  
The United States ◽  
Transgenic Crop ◽  
Nptii Gene

Abstract The use of transgenic crops has become increasingly common in the United States over the last several decades. Increasing evidence suggests that DNA may be protected from enzymatic digestion and acid hydrolysis in the digestive tract, suggesting that crop-derived transgenes may enter into wastewater treatment plants (WWTPs) intact. Given the historical use of antibiotic resistance genes as selection markers in transgenic crop development, it is important to consider the fate of these transgenes. Herein we detected and quantified crop-derived transgenes in WWTPs. All viable US WWTP samples were found to contain multiple gene targets (p35, nos, bla and nptII) at significantly higher levels than control samples. Control wastewater samples obtained from France, where transgenic crops are not cultivated, contained significantly fewer copies of the nptII gene than US activated and digester sludges. No significant differences were measured for the bla antibiotic resistance gene (ARG). In addition, a nested PCR (polymerase chain reaction) assay was developed that targeted the bla ARG located in regions flanked by the p35 promoter and nos terminator. Overall this work suggests that transgenic crops may have provided an environmental source of nptII; however, follow-up studies are needed to ascertain the viability of these genes as they exit WWTPs.

scholarly journals Quantitative Occurrence of Antibiotic Resistance Genes among Bacterial Populations from Wastewater Treatment Plants Using Activated Sludge

2019 ◽  
Vol 9 (3) ◽  
pp. 387 ◽  
Author(s):  
Adriana Osińska ◽  
Ewa Korzeniewska ◽  
Monika Harnisz ◽  
Sebastian Niestępski
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Gene ◽  
Antibiotic Resistance Genes ◽  
Treated Wastewater ◽  
Beta Lactams ◽  
Treatment Technology ◽  
Bacterial Populations

Wastewater treatment plants (WWTPs) are an important reservoir in the development of drug resistance phenomenon and they provide a potential route of antibiotic resistance gene (ARGs) dissemination in the environment. The aim of this study was to assess the role of WWTPs in the spread of ARGs. Untreated and treated wastewater samples that were collected from thirteen Polish WWTPs (applying four different modifications of activated sludge–based treatment technology) were analyzed. The quantitative occurrence of genes responsible for the resistance to beta-lactams and tetracyclines was determined using the real-time PCR method. Such genes in the DNA of both the total bacterial population and of the E. coli population were analyzed. Among the tested genes that are responsible for the resistance to beta-lactams and tetracyclines, blaOXA and blaTEM and tetA were dominant, respectively. This study found an insufficient reduction in the quantity of the genes that are responsible for antibiotic resistance in wastewater treatment processes. The results emphasize the need to monitor the presence of genes determining antibiotic resistance in the wastewater that is discharged from treatment plants, as they can help to identify the hazard that treated wastewater poses to public health.

scholarly journals Shift in antibiotic resistance gene profiles associated with nanosilver during wastewater treatment

2016 ◽  
Vol 92 (3) ◽  
pp. fiw022 ◽  
Author(s):  
Yanjun Ma ◽  
Jacob W. Metch ◽  
Ying Yang ◽  
Amy Pruden ◽  
Tong Zhang
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Gene ◽  
Antibiotic Resistance Gene ◽  
Gene Profiles

scholarly journals The Effect of Primary, Secondary, and Tertiary Wastewater Treatment Processes on Antibiotic Resistance Gene (ARG) Concentrations in Solid and Dissolved Wastewater Fractions

Water ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 37 ◽  
Author(s):  
Jennipher Quach-Cu ◽  
Bellanira Herrera-Lynch ◽  
Christine Marciniak ◽  
Scott Adams ◽  
April Simmerman ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Gene ◽  
Antibiotic Resistance Gene ◽  
Treatment Processes

scholarly journals High levels of antibiotic resistance gene expression among birds living in a wastewater treatment plant

2018 ◽  
Author(s):  
Vanessa R. Marcelino ◽  
Michelle Wille ◽  
Aeron C. Hurt ◽  
Daniel González-Acuña ◽  
Marcel Klaassen ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Wastewater Treatment Plant ◽  
Antibiotic Resistance Gene ◽  
Treatment Plant ◽  
Antibiotic Resistance Genes ◽  
Resistant Bacteria ◽  
Chloramphenicol Resistance

AbstractAntibiotic resistance is rendering common bacterial infections untreatable. Wildlife can incorporate and disperse antibiotic resistant bacteria in the environment, such as water systems, which in turn serve as reservoirs of resistance genes for human pathogens. We used bulk RNA-sequencing (meta-transcriptomics) to assess the diversity and expression levels of functionally active resistance genes in the microbiome of birds with aquatic behavior. We sampled birds across a range of habitats, from penguins in Antarctica to ducks in a wastewater treatment plant in Australia. This revealed 81 antibiotic resistance genes in birds from all localities, including β-lactam, tetracycline and chloramphenicol resistance in Antarctica, and genes typically associated with multidrug resistance plasmids in areas with high human impact. Notably, birds feeding at a wastewater treatment plant carried the greatest resistance gene burden, suggesting that human waste, even if it undergoes treatment, contributes to the spread of antibiotic resistance genes to the wild. Differences in resistance gene burden also reflected the birds’ ecology, taxonomic group and microbial functioning. Ducks, which feed by dabbling, carried a higher abundance and diversity of resistance genes than turnstones, avocets and penguins, that usually prey on more pristine waters. In sum, this study helps to reveal the complex factors explaining the distribution of resistance genes and their exchange routes between humans and wildlife.

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