scholarly journals Growth and gonadal development retardations after long-term exposure to estradiol in little yellow croaker, Larimichthys polyactis

2021 ◽  
Vol 222 ◽  
pp. 112462
Author(s):  
Qing-Ping Xie ◽  
Bing-Bing Li ◽  
Fu-Liang Wei ◽  
Min Yu ◽  
Wei Zhan ◽  
...  
Keyword(s):  
Gonadal Development ◽  
Larimichthys Polyactis

Roles of gonad-inhibiting hormone in the protandric simultaneous hermaphrodite peppermint shrimp†

2020 ◽  
Vol 103 (4) ◽  
pp. 817-827
Author(s):  
Wenyuan Shi ◽  
Fang Liu ◽  
An Liu ◽  
Huiyang Huang ◽  
Qi Lin ◽  
...  
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Gonadal Development ◽  
Simultaneous Hermaphrodite ◽  
Secondary Sexual Characteristic ◽  
Significant Difference ◽  
Peppermint Shrimp ◽  
Gonad Inhibiting Hormone ◽  
Sexual Characteristic ◽  
Secondary Sexual

Abstract To date, the molecular mechanisms of the unique gonadal development mode known as protandric simultaneous hermaphroditism (PSH) are unclear in crustaceans. In this study, cDNA of a gonad-inhibiting hormone (Lv-GIH1) was isolated from the PSH peppermint shrimp Lysmata vittata, and its expression was exclusively found in the eyestalk ganglion. Real-time quantitative polymerase chain reaction (qRT-PCR) revealed that the expression of Lv-GIH1 increased during gonadal development of the functional male stages but decreased significantly at subsequent simultaneous hermaphroditism stage. Further in vitro experiment showed that recombinant GIH1 protein (rGIH1) effectively inhibited Vg expression in the cultured hepatopancreas tissues while the short-term injection of GIH1-dsRNA resulted in reduced expression of Lv-GIH1 and upregulated expression of Vg in the hepatopancreas. Moreover, long-term rGIH1 injection led to significantly reduced expression of Lv-Vg, Lv-VgR, and Lv-CFSH1, subdued growth of oocytes, and feathery setae as a secondary sexual characteristic in females. Interestingly, while germ cells in testicular part were suppressed by rGIH1 injection, the expression of Lv-IAGs showed no significant difference; and long-term GIH1-dsRNA injection results were contrary to those of rGIH1 injection. Taken together, the results of this study indicate that Lv-GIH1 is involved in gonadal development and might also participate in controlling secondary sexual characteristic development in L. vittata by inhibiting Lv-CFSH1 expression.

Beberapa Aspek Reproduksi Siput Lambis lambis di Pesisir Perairan Yenusi, Biak

2016 ◽  
Vol 1 (3) ◽  
pp. 1
Author(s):  
Andriani Widyastuti ◽  
Ludi Parwadani Aji
Keyword(s):  
Sex Ratio ◽  
Management Strategies ◽  
Gonadal Development ◽  
Shell Size ◽  
Gonad Maturity ◽  
Males And Females ◽  
Over Exploitation ◽  
Number Of Males ◽  
Time Of Harvest

<strong>Some Aspects of Reproduction in Conch Lambis lambis of Yenusi Coastal Waters, Biak.</strong> Lambis lambis is a very popular conch species that has been harvested in all sizes encountered. This condition is very alarming because in the long term, its existence in nature will be reduced because there is no chance to reproduce naturally. This study aims to determine the sex ratio and gonad maturity levels of L. lambis in Yenusi waters, so the appropriate time of harvest can be arranged. Samples were collected every month from January to December 2013. Testing and histological analysis were performed in the laboratory. Samples collected were 99 individus with the number of males 45 individus and females 54 individus. The sex ratio of males and females was 1.0:1.2. Gonad maturity level found included all four stages of gonadal development, from TKG I to TKG IV. Gonadal development showed the process of formation and maturation, and the spawning occured throughout the year, with peak spawning in January until March 2013. The size of the shell length obtained ranged from 4.55 to 13.72 cm indicating over-exploitation. Due to the custom local where residents harvested the conches in all sizes found, sustainable management strategies are urgently needed, including moratorium period  when L. lambis is at the peak of spawning, and harvest should only be performed on adult individuals with a minimum shell size of 7 cm, while observing the thickness of the shell and marginal digitations have been formed. <br /><br />

scholarly journals Steroidogenic factor 1/adrenal 4 binding protein transforms human bone marrow mesenchymal cells into steroidogenic cells

2007 ◽  
Vol 39 (5) ◽  
pp. 343-350 ◽  
Author(s):  
Tomoko Tanaka ◽  
Shigeki Gondo ◽  
Taijiro Okabe ◽  
Kenji Ohe ◽  
Hisao Shirohzu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Binding Protein ◽  
Gonadal Development ◽  
Steroidogenic Factor 1 ◽  
Steroid Profile ◽  
Steroidogenic Cells ◽  
Gland Development ◽  
Bone Marrow Mesenchymal Cells

AbstractSteroidogenic factor 1/adrenal 4 binding protein (SF-1/Ad4BP) is an essential nuclear receptor for steroidogenesis as well as for adrenal and gonadal gland development. Mesenchymal bone marrow cells (BMCs) contain pluripotent progenitor cells, which differentiate into multiple lineages. In a previous study, we reported that adenovirus-mediated forced expression of SF-1 could transform mouse primary long-term cultured BMCs into steroidogenic cells. For future clinical application, trials using human BMCs would be indispensable. In this study, we examined whether SF-1 could transform human BMCs into steroidogenic cells and compared the steroid profile of these cellswith that of mouse steroidogenic BMCs. Primary cultured human BMCs infected with adenovirus containing bovine SF-1 cDNA could produce progesterone, corticosterone, cortisol, dehydroepiandrosterone, testosterone, and estradiol. Such a mixed character of adrenal and gonadal steroid production in human BMCs was supported by the expressions of P450scc, 3β-hydroxysteroid dehydrogenase (3β-HSD), P450c21, P450c11, P450c17, 17β-HSD, and P450arom mRNAs. Unlike mouse steroidogenic BMCs, introduction of SF-1 into human BMCs caused dramatic inductions of both ACTH and LH receptors, thus leading to good responsiveness of the cells to ACTH and LH respectively. Importantly, among several factors that are known to be closely associated with adrenal and/or gonadal development, introduction of only SF-1 enabled the human BMCs to express P450scc and to produce cortisol and testosterone, suggesting that SF-1 is truly a master regulator for the production of steroidogenic cells from human BMCs.

scholarly journals A Transient Hermaphroditic Stage in Early Male Gonadal Development in Little Yellow Croaker, Larimichthys polyactis

2021 ◽  
Vol 11 ◽  
Author(s):  
Qing-Ping Xie ◽  
Bing-Bing Li ◽  
Wei Zhan ◽  
Feng Liu ◽  
Peng Tan ◽  
...  
Keyword(s):  
Germ Cells ◽  
Evolutionary Relationship ◽  
Gonadal Development ◽  
Ovary Development ◽  
Larimichthys Polyactis ◽  
Efferent Duct ◽  
Sequential Hermaphroditism ◽  
Serum Hormone ◽  
Early Primary ◽  
Male Specific

Animal taxa show remarkable variability in sexual reproduction, where separate sexes, or gonochorism, is thought to have evolved from hermaphroditism for most cases. Hermaphroditism accounts for 5% in animals, and sequential hermaphroditism has been found in teleost. In this study, we characterized a novel form of the transient hermaphroditic stage in little yellow croaker (Larimichthys polyactis) during early gonadal development. The ovary and testis were indistinguishable from 7 to 40 days post-hatching (dph). Morphological and histological examinations revealed an intersex stage of male gonads between 43 and 80 dph, which consist of germ cells, somatic cells, efferent duct, and early primary oocytes (EPOs). These EPOs in testis degenerate completely by 90 dph through apoptosis yet can be rescued by exogenous 17-β-estradiol. Male germ cells enter the mitotic flourishing stage before meiosis is initiated at 180 dph, and they undergo normal spermatogenesis to produce functional sperms. This transient hermaphroditic stage is male-specific, and the ovary development appears to be normal in females. This developmental pattern is not found in the sister species Larimichthys crocea or any other closely related species. Further examinations of serum hormone levels indicate that the absence of 11-ketotestosterone and elevated levels of 17-β-estradiol delineate the male intersex gonad stage, providing mechanistic insights on this unique phenomenon. Our research is the first report on male-specific transient hermaphroditism and will advance the current understanding of fish reproductive biology. This unique gonadal development pattern can serve as a useful model for studying the evolutionary relationship between hermaphroditism and gonochorism, as well as teleost sex determination and differentiation strategies.

scholarly journals Long-term changes in the small yellow croaker, Larimichthys polyactis, population in the Yellow and East China Seas

2010 ◽  
Vol 46 (4) ◽  
pp. 392-405 ◽  
Author(s):  
In-Ja Yeon ◽  
Dong-Woo Lee ◽  
Jae-Bong Lee ◽  
Kwang-Ho Choi ◽  
Byung-Kyu Hong ◽  
...  
Keyword(s):  
East China ◽  
China Seas ◽  
Larimichthys Polyactis ◽  
Small Yellow Croaker ◽  
Long Term Changes

scholarly journals Long-term restoration of gonadal activity with xenografts of preoptic area tissue in hypogonadal (hpg) mice

1998 ◽  
Vol 72 (3) ◽  
pp. 229-236 ◽  
Author(s):  
M. J. A. WOOD ◽  
K. J. WOOD ◽  
H. M. CHARLTON
Keyword(s):  
Preoptic Area ◽  
Gonadal Development ◽  
Ovarian Follicles ◽  
Seminal Vesicles ◽  
Mutant Mice ◽  
Phenotypic Characterization ◽  
Gonadal Activity ◽  
Testicular Weight ◽  
Original Observation

The identification of novel mutants and their genetic and phenotypic characterization leads initially to fairly well-defined areas of experimentation. However, some mutant models lend themselves to investigations in fields that at first glance may appear remote from the original observation.This has been true of the hypogonadal (hpg) mouse first discovered by Bruce Cattanach (Cattanach et al., 1977). In these mutant mice there is a failure of postnatal gonadal development such that paired testicular weight in 60-d-old hpg males is less than 10 mg whilst in normal littermates the testes weigh nearly 200 mg. The seminal vesicles of the mutants are extremely atrophic, indicating a failure of androgen production by the testes. In female mutants ovarian follicles rarely advance beyond the pre-antral stage and the uterus is thin and thread-like.

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