Boron application mitigates Cd toxicity in leaves of rice by subcellular distribution, cell wall adsorption and antioxidant system

Abstract Background Cadmium (Cd) accumulation in crops affects the yield and quality of crops and harms human health. The application of selenium (Se) can reduce the absorption and transport of Cd in winter wheat. Results The results showed that increasing Se supply significantly decreased Cd concentration and accumulation in the shoot and root of winter wheat and the root-to-shoot translocation of Cd. Se application increased the root length, surface area and root volume but decreased the average root diameter. Increasing Se supply significantly decreased Cd concentration in the cell wall, soluble fraction and cell organelles in root and shoot. An increase in Se supply inhibited Cd distribution in the organelles of shoot and root but enhanced Cd distribution in the soluble fraction of shoot and the cell wall of root. The Se supply also decreased the proportion of active Cd (ethanol-extractable (FE) Cd and deionized water-extractable (FW) Cd) in root. In addition, the expression of TaNramp5-a, TaNramp5-b, TaHMA3-a, TaHMA3-b and TaHMA2 significantly increased with increasing Cd concentration in root, and the expression of TaNramp5-a, TaNramp5-b and TaHMA2 in root was downregulated by increasing Se supply, regardless of Se supply or Cd stress. The expression of TaHMA3-b in root was significantly downregulated by 10 μM Se at both the 5 μM and 25 μM Cd level but upregulated by 5 μM Se at the 25 μM Cd level. The expression of TaNramp5-a, TaNramp5-b, TaHMA3-a, TaHMA3-b and TaHMA2 in shoot was downregulated by increasing Se supply at 5 μM Cd level, and 5 μM Se upregulated the expression of those genes in shoot at 25 μM Cd level. Conclusions The results confirm that Se application limits Cd accumulation in wheat by regulating the subcellular distribution and chemical forms of Cd in winter wheat tissues, as well as the expression of TaNramp5-a, TaNramp5-b and TaHMA2 in root.

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Impact of hot water‐calcium on the activity of cell wall degrading and antioxidant system enzymes in mango stored at chilling temperature

Journal of Food Biochemistry ◽

10.1111/jfbc.13286 ◽

2020 ◽

Vol 44 (8) ◽

Cited By ~ 2

Author(s):

Denisse Aurora Díaz‐Corona ◽

Martha Edith López‐López ◽

Lidia Elena Ayón‐Reyna ◽

Jordi Gerardo López‐Velázquez ◽

Blanca Alicia López‐Zazueta ◽

...

Keyword(s):

Cell Wall ◽

Antioxidant System ◽

Hot Water ◽

Chilling Temperature

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Differences in Mn uptake and subcellular distribution in different barley genotypes as a response to Cd toxicity

The Science of The Total Environment ◽

10.1016/j.scitotenv.2006.02.016 ◽

2007 ◽

Vol 385 (1-3) ◽

pp. 228-234 ◽

Cited By ~ 17

Author(s):

F WU ◽

J DONG ◽

Y CAI ◽

F CHEN ◽

G ZHANG

Keyword(s):

Subcellular Distribution ◽

Cd Toxicity ◽

Barley Genotypes

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Foliar application of Zn reduces Cd accumulation in grains of late rice by regulating the antioxidant system, enhancing Cd chelation onto cell wall of leaves, and inhibiting Cd translocation in rice

The Science of The Total Environment ◽

10.1016/j.scitotenv.2021.145302 ◽

2021 ◽

Vol 770 ◽

pp. 145302

Author(s):

Sheng Zhen ◽

Hong Shuai ◽

Chao Xu ◽

Guanghui Lv ◽

Xiangdong Zhu ◽

...

Keyword(s):

Cell Wall ◽

Antioxidant System ◽

Foliar Application ◽

Cd Accumulation ◽

Cd Translocation ◽

Late Rice

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Exogenous Glutathione Alleviation of Cd Toxicity in Italian Ryegrass (Lolium multiflorum) by Modulation of the Cd Absorption, Subcellular Distribution, and Chemical Form

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17218143 ◽

2020 ◽

Vol 17 (21) ◽

pp. 8143

Author(s):

Zhigang Fang ◽

Zhaoyang Hu ◽

Xinqiang Yin ◽

Gang Song ◽

Qingsheng Cai

Keyword(s):

Cell Wall ◽

Soluble Fraction ◽

Lolium Multiflorum ◽

Chemical Form ◽

Italian Ryegrass ◽

Chemical Forms ◽

Cd Uptake ◽

Cd Toxicity ◽

Low Toxicity ◽

Cd Translocation

Subcellular fractions and the chemical forms of cadmium (Cd) reflect its level of toxicity to plants; however, these effects of exogenous glutathione (GSH) are poorly understood. We exposed two Italian ryegrass (Lolium multiflorum) cultivars (IdyII and Harukaze) to 50 µM Cd or 200 µM GSH to investigate the effect of GSH on the Cd uptake, subcellular compartments, and chemical forms. Cd significantly inhibited the plant growth, while GSH supplementation decreased this inhibition. The application of GSH significantly improved the Cd concentration in the roots but reduced that in the shoots and decreased the Cd translocation from root to shoot. The Cd concentration of the root in the cell wall was increased while the concentration in the soluble fraction was decreased when supplied with GSH. The inorganic form (80% ethanol for Cd extraction) in the roots was significantly reduced when treated with GSH. The Cd form extracted by 2% acetic acid (HAC) with low toxicity and immobility were greatly increased. In leaves, the application GSH decreased in any form of Cd form extracted. In conclusion, exogenous GSH decreased the translocation of Cd and alleviated Italian ryegrass Cd toxicity by accumulating more Cd in the root cell wall and immobilizing more Cd in lower toxicity fractions.

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