Triadimefon increases fetal Leydig cell proliferation but inhibits its differentiation of male fetuses after gestational exposure

Lepidium meyenii is now widely consumed as a functional food and medicinal product, which is known as an enhancer of reproductive health. However, the specific chemical composition and mechanism of action for improving sexual function are unclear. The present study aims at screening and determining the potential compounds, which promote mouse leydig cells (TM3) proliferation. The partial least squares analysis (PLS) was employed to reveal the correlation between common peaks of high performance liquid chromatography (HPLC) fingerprint of L. meyenii and the proliferation activity of TM3. The results suggested that three compounds had good activities on the proliferation of TM3 and promoting testosterone secretion, there were N-benzyl-hexadecanamide, N-benzyl-(9z,12z)-octadecadienamide and N-benzyl-(9z,12z,15z)-octadecatrienamide which might be the potential bioactive markers related to the enhancing sexual ability functions of L. meyenii. The first step in testosterone synthesis is the transport of cholesterol into the mitochondria, and the homeostasis of mitochondrial function is related to cyclophilin D (CypD). In order to expound how bioactive ingredients lead to promoting testosterone secretion, a molecular docking simulation was used for further illustration in the active sites and binding degree of the ligands on CypD. The results indicated there was a positive correlation between the binding energy absolute value and testosterone secretion activity. In addition, in this study it also provided the reference for a simple, quick method to screen the promoting leydig cell proliferation active components in traditional Chinese medicine (TCM).

Download Full-text

Temperature and germ cell regulation of Leydig cell proliferation stimulated by Sertoli cell-secreted mitogenic factor: a possible role in cryptorchidism

Andrologia ◽

10.1111/j.1439-0272.1996.tb02792.x ◽

2009 ◽

Vol 28 (5) ◽

pp. 247-257 ◽

Cited By ~ 12

Author(s):

N. Wu ◽

E. P. Murono

Keyword(s):

Cell Proliferation ◽

Germ Cell ◽

Sertoli Cell ◽

Leydig Cell ◽

Cell Regulation ◽

Mitogenic Factor

Download Full-text

Evidence that α5β1 integrins mediate Leydig cell binding to fibronectin and enhance Leydig cell proliferation stimulated by a Sertoli cell-secreted mitogenic factor in vitro

Endocrine ◽

10.1007/bf02738659 ◽

1996 ◽

Vol 5 (1) ◽

pp. 75-83 ◽

Cited By ~ 5

Author(s):

Naixing Wu ◽

Eisuke P. Murono ◽

Wayne E. Carver ◽

Louis Terracio ◽

Thierry Bacro

Keyword(s):

Cell Proliferation ◽

Sertoli Cell ◽

Leydig Cell ◽

Cell Binding ◽

Mitogenic Factor

Download Full-text

The Production of Testosterone and Gene Expression in Neonatal Testes of Rats Exposed to Diisoheptyl Phthalate During Pregnancy is Inhibited

Frontiers in Pharmacology ◽

10.3389/fphar.2021.568311 ◽

2021 ◽

Vol 12 ◽

Author(s):

Bin Ji ◽

Zina Wen ◽

Chaobo Ni ◽

Qiqi Zhu ◽

Yiyan Wang ◽

...

Keyword(s):

Leydig Cell ◽

Corn Oil ◽

Serum Testosterone ◽

Male Rats ◽

Sprague Dawley ◽

Testosterone Levels ◽

Protein Levels ◽

Gestational Exposure ◽

Phthalate Plasticizer ◽

Neonatal Testis

Background: Diisoheptyl phthalate (DIHP) is a phthalate plasticizer, which is a branched phthalate. Here, we reported the effects of gestational exposure to DIHP on testis development in male rats.Methods: Pregnant Sprague-Dawley rats were orally fed with vehicle (corn oil, control) or DIHP (10, 100, 500, and 1,000 mg/kg) from gestational day (GD) 12–21. At GD21, serum testosterone levels, the number and distribution of fetal Leydig cells, and testicular mRNA and protein levels, the incidence of multinucleated gonocytes, and focal testicular hypoplasia in the neonatal testis were measured.Results: DIHP increased the fetal Leydig cell cluster size and decreased the fetal Leydig cell size with LOAEL of 10 mg/kg. DIHP did not affect the fetal Leydig cell number. DIHP significantly lowered serum testosterone levels, down-regulated the expression of steroidogenesis-related genes (Lhcgr, Star, Cyp11a1, Hsd3b1, Cyp17a1, and Hsd17b3) and testis descent-related gene (Insl3) as well as protein levels of cholesterol side-chain cleavage enzyme (CYP11A1) and insulin-like 3 (INSL3). DIHP dose-dependently increased the percentage of multinucleated gonocytes with the low observed adverse-effect level (LOAEL) of 100 mg/kg. DIHP induced focal testicular hypoplasia.Conclusion: Gestational exposure to DIHP causes testis dysgenesis in rats.

Download Full-text