Usefulness of Genotype MTBDRplus assay in acid-fast bacilli positive smear specimens in Almeria, Spain

2014 ◽  
Vol 32 (8) ◽  
pp. 511-514 ◽  
Author(s):  
Miguel José Martínez-Lirola ◽  
María José Munoz-Dávila ◽  
Darío García-de Viedma ◽  
Teresa Cabezas Fernández ◽  
Pilar Luzón García
e-CliniC ◽  
2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Diana Susanti

Abstrack: Cough ≥ 2 weeks are the main symptoms of tuberculosis diagnosis accompanied by phlegm.Symptoms that accompany the cough bleed, decreased appetite,weight loss, night sweats (no activity) and no signs of infection. Tuberculosis is a disease of the lungs caused by gram-positive rod bacterium (Mycobakterium tuberculosis). This research is a descriptive nature experiment is to determine the presence of bacteria mycobacterium tuberculosis in sputum of patients cough ≥ 2 weeks in the Internal Medicine Clinic Dr BLU. Prof. Dr. Kandou with smear using Ziehl Neelsen.The results are 50 samples, 23 (46%) positive and 27 (54%) negative.Presentation by gender with the most positive smear on male sex 65.2% from than 34.8% of women.Presentation of the age group with the highest incidence of smear positive cases in the age group 55-74 years old 39.3%. Conclusion: Examination of acid fast bacilli in the sputum of patients cough ≥ 2 weeks needs to be done in order to determine the existence of bacteria mycobacterium tuberculosis.Tuberculosis is most common in males than in females in the age group 55-74 years old.Keyword: cough more than two weeks, tuberculosis, BTA.Abstrak: Batuk ≥ 2 minggu merupakan gejala utama diagnosis penyakit tuberkulosis yang disertai dengan dahak. Gejala yang menyertai yaitu batuk yang mengeluarkan darah, nafsu makan menurun, penurunan berat badan, keringat pada malam hari (tanpa aktifitas) dan ada tanda-tanda infeksi.Tuberkulosis paru ini adalah penyakit yang menyerang paru yang disebabkan oleh bakteri batang gram positif (Mycobakterium tuberkulosis).Penelitian ini merupakan penelitian deskritif bersifat ekperimen yaitu untuk mengetahui adanya kuman mycobacterium tuberkulosis pada sputum penderita batuk ≥ 2 minggu di Poliklinik Penyakit Dalam BLU RSUP.Prof. Dr. Kandou dengan di lakukan pemeriksaan BTA mengunakan pewarnaan ziehl neelsen.Hasil penelitian terdapat 50 sampel, 23 (46%) positif dan 27 (54%) negatif. Presentasi berdasarkan jenis kelamin dengan bta positif terbanyak pada jenis kelamin laki-laki 65,2% daripada perempuan 34,8%. Presentasi kelompok umur dengan bta positif terbanyak terjadi pada kelompok umur 55 – 74 tahun 39,3%. Simpulan: Pemeriksaan basil tahan asam (BTA) pada sputum penderita batuk ≥ 2 minggu perlu dilakukan agar mengetahui adanya kuman mycobacterium tuberkulosis. Tuberkulosis paling sering terjadi pada laki-laki dibandingkan perempuan pada kelompok umur 55 - 74 tahun.Kata Kunci: batuk lebih dari 2 minggu, tuberkulosis, BTA.


2012 ◽  
Vol 4 (5) ◽  
pp. 231 ◽  
Author(s):  
TaghiNaserpour Farivar ◽  
Pouran Johari ◽  
AbbasAli Moien ◽  
MohammadHashemi Shahri ◽  
Mohammad Naderi ◽  
...  

2015 ◽  
Vol 13 (4) ◽  
pp. 308-314 ◽  
Author(s):  
Alfred Nwofor ◽  
Amaase Nyamngee ◽  
Charles Nwabuisi ◽  
Mosunmola Iwakun ◽  
Masur Gidado ◽  
...  
Keyword(s):  

2020 ◽  
Vol 20 (4) ◽  
pp. 491-494 ◽  
Author(s):  
Eltayib H. Ahmed Abakur ◽  
Tarig M.S. Alnour ◽  
Faisel Abuduhier ◽  
Fahad M.A. Albalawi ◽  
Khalid A.S. Alfifi

Purpose: Heteroresistant Mycobacterium tuberculosis (MTB) is defined as a group of drug-susceptible and resistant bacteria in a single clinical specimen from tuberculosis (TB) patients. Heteroresistance of MTB is considered a preliminary stage to full resistance. The present study aimed to determine the heteroresistance in Mycobacterium tuberculosis in Tabuk province, in the north of the Kingdom of Saudi Arabia. Method: GenoType MTBDRplus assay was used to determine mutations associated with isoniazid and rifampicin resistance. Results: A total number of 46 confirmed M. tuberculosis positive sputum samples were scanned for heteroresistance. The present study revealed 3 (6.5%) heteroresistant mutations to either rpoB gene alone, 2 (4.4%) to rpoB and 1 (2.2%) to inhA genes. Conclusion: The detection of heteroresistant mutations could guide the initiation of an appropriate regimen of treatment.


2021 ◽  
Vol 32 (5) ◽  
pp. 483-485
Author(s):  
Yo Murata ◽  
Nobuaki Mori ◽  
Narito Kagawa ◽  
Kentaro Okuma ◽  
Shinji Yoshida ◽  
...  

Mycobacterium genavense, a nontuberculous Mycobacterium, is found in immunosuppressed patients, particularly in those with HIV. Mycobacterium genavense incubation under standard culture conditions is difficult, and its identification is challenging using routine culture methods. Herein, we report the case of a 40-year-old Japanese man with HIV presenting with disseminated M. genavense infection. An analysis using an automated blood culture system did not show positive signals during 6 weeks of incubation. However, an acid-fast bacilli smear of his blood sample was positive for the bacterium. Mycobacterium genavense was identified using sequencing analysis, targeting the heat shock protein 65 gene. The patient recovered from the infection, following antibiotic therapy for 18 months. Under suspicion of disseminated M. genavense infection and the absence of bacterial growth in blood culture samples, an acid-fast bacilli smear test of the sample may be useful for timely diagnosis.


2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Diana Machado ◽  
Jorge Ramos ◽  
Isabel Couto ◽  
Nureisha Cadir ◽  
Inácio Narciso ◽  
...  

We evaluate the performance of the TBcID assay in a panel of 100 acid-fast bacilli cultures. Sixty-four isolates were TBcID positive forMycobacterium tuberculosiscomplex (MTBC), whereas 36 gave negative results. These included 28 nontuberculous mycobacteria, one nonmycobacterial isolate, oneM. tuberculosis, and sixM. bovisBCG strains. This corresponds to a sensitivity of 90.14%, specificity of 100%, and positive and negative predictive values of 100% and 80.55%, respectively. The test is rapid, easy to perform and interpret, and does not require sample preparation or instrumentation. However, a negative result does not exclude the presence of a strain belonging to MTBC, especially when mutations inmpb64gene are present or someM. bovisBCG strains are isolated. The TBcID showed potential to assist in the identification of MTBC when the implementation and usage of molecular methods are often not possible, principally in resource-limited countries.


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