Characterization of NDM-1- and CMH-3-producing Enterobacter cloacae complex ST932 in a patient transferred from Ukraine to Spain

2020 ◽  
Vol 38 (7) ◽  
pp. 327-330
Author(s):  
Inmaculada López-Hernández ◽  
Aurora García Barrionuevo ◽  
Paula Díaz de Alba ◽  
Encarnación Clavijo ◽  
Alvaro Pascual
Keyword(s):  
Enterobacter Cloacae ◽  
Enterobacter Cloacae Complex

scholarly journals Genomic Study of blaIMI-Positive Enterobacter cloacae Complex in Singapore over a Five-Year Study Period

2020 ◽  
Vol 64 (8) ◽  
Author(s):  
Sophie Octavia ◽  
Tse Hsien Koh ◽  
Oon Tek Ng ◽  
Kalisvar Marimuthu ◽  
Indumathi Venkatachalam ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Mobile Elements ◽  
Content Type ◽  
Genomic Study ◽  
Genomic Characterization ◽  
Enterobacter Cloacae Complex

ABSTRACT The blaIMI gene is rarely detected outside the Enterobacter genus. Genomic characterization of 87 blaIMI-positive Enterobacter cloacae complex members revealed that the largest phylogenomic clade was made up of E. cloacae subsp. cloacae (71.3%), followed by the newly described species E. bugandensis (13.8%), E. sichuanensis (10.3%), and E. roggenkampii (4.6%). IMI-1 was the predominant carbapenemase variant (86/87, 98.9%). All the blaIMI genes were associated with chromosomally integrated Xer-dependent integrative mobile elements (IMEXs), with two new variants detected.

scholarly journals Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 79
Author(s):  
Vittoria Mattioni Marchetti ◽  
Ibrahim Bitar ◽  
Mario Sarti ◽  
Elena Fogato ◽  
Erika Scaltriti ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Sequencing Technology ◽  
Genomic Features ◽  
Long Reads ◽  
Clinical Resistance ◽  
Genomic Characterization ◽  
Enterobacteriaceae Infections ◽  
Enterobacter Cloacae Complex ◽  
Conjugation Experiment

Background: the co-production of carbapenemases and mcr-genes represents a worrisome event in the treatment of Enterobacteriaceae infections. The aim of the study was to characterize the genomic features of two clinical Enterobacter cloacae complex (ECC) isolates, co-producing VIM and MCR enzymes, in Italy. Methods: species identification and antibiotic susceptibility profiling were performed using MALDI-TOF and broth microdilution methods, respectively. Transferability of the blaVIM- and mcr- type genes was verified through conjugation experiment. Extracted DNA was sequenced using long reads sequencing technology on the Sequel I platform (PacBio). Results: the first isolate showed clinical resistance against ertapenem yet was colistin susceptible (EUCAST 2020 breakpoints). The mcr-9.2 gene was harbored on a conjugative IncHI2 plasmid, while the blaVIM-1 determinant was harbored on a conjugative IncN plasmid. The second isolate, resistant to both carbapenems and colistin, harbored: mcr-9 gene and its two component regulatory genes for increased expression on the chromosome, mcr-4.3 on non-conjugative (yet co-transferable) ColE plasmid, and blaVIM-1 on a non-conjugative IncA plasmid. Conclusions: to our knowledge, this is the first report of co-production of VIM and MCR in ECC isolates in Italy.

scholarly journals Molecular Characterization of KPC-2-Producing Enterobacter cloacae Complex Isolates from Cali, Colombia

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 694
Author(s):  
Aura Falco ◽  
Daniela Guerrero ◽  
Isabella García ◽  
Adriana Correa ◽  
Sandra Rivera ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Public Health Problem ◽  
Carbapenem Resistance ◽  
Enterobacter Cloacae ◽  
Epidemiological Surveillance ◽  
Beta Lactam ◽  
High Genetic Diversity ◽  
Effective Prevention ◽  
Enterobacter Cloacae Complex

The Enterobacter cloacae complex is an emerging opportunistic pathogen whose increased resistance to carbapenems is considered a public health problem. This is due to the loss of efficacy of beta-lactam antibiotics, which are used as the first treatment option in the management of infections caused by Gram-negative bacteria. The objective of this study was to perform the molecular characterization of 28 isolates of the E. cloacae complex resistant to cephalosporins and carbapenems isolated between 2011 and 2018 from five hospitals located in the municipality of Santiago de Cali, Colombia. Molecular detection of blaKPC, blaVIM, blaNDM and blaOXA-48-like genes was performed on these isolates and the genetic relationship between the isolates was assessed using multilocus sequence typing (MLST). Forty-three percent of the isolates carried the blaKPC-2 gene variant. MLST showed high genetic diversity among isolates, the most frequent being the sequence type ST510 with a frequency of 50%. The identification of the genes involved in carbapenem resistance and dispersing genotypes is an important step toward the development of effective prevention and epidemiological surveillance strategies in Colombian hospitals.

Characterization of NDM-1- and CMH-3-producing Enterobacter cloacae complex ST932 in a patient transferred from Ukraine to Spain

2020 ◽  
Vol 38 (7) ◽  
pp. 327-330 ◽  
Author(s):  
Inmaculada López-Hernández ◽  
Aurora García Barrionuevo ◽  
Paula Díaz de Alba ◽  
Encarnación Clavijo ◽  
Alvaro Pascual
Keyword(s):  
Enterobacter Cloacae ◽  
Enterobacter Cloacae Complex

scholarly journals Characterization of resistance mechanisms of Enterobacter cloacae Complex co-resistant to carbapenem and colistin

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shixing Liu ◽  
Renchi Fang ◽  
Ying Zhang ◽  
Lijiang Chen ◽  
Na Huang ◽  
...  
Keyword(s):  
Lipid A ◽  
Efflux Pump ◽  
Resistance Mechanism ◽  
Carbapenem Resistance ◽  
Enterobacter Cloacae ◽  
Resistance Mechanisms ◽  
Colistin Resistance ◽  
Carbapenem Resistant ◽  
Horizontal Spread

Abstract Background The emergence of carbapenem-resistant and colistin-resistant ECC pose a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in Enterobacter cloacae Complex (ECC) strains. Results This study showed that the mechanisms of carbapenem resistance in this study are: 1. Generating carbapenemase (7 of 19); 2. The production of AmpC or ESBLs combined with decreased expression of out membrane protein (12 of 19). hsp60 sequence analysis suggested 10 of 19 the strains belong to colistin hetero-resistant clusters and the mechanism of colistin resistance is increasing expression of acrA in the efflux pump AcrAB-TolC alone (18 of 19) or accompanied by a decrease of affinity between colistin and outer membrane caused by the modification of lipid A (14 of 19). Moreover, an ECC strain co-harboring plasmid-mediated mcr-4.3 and blaNDM-1 has been found. Conclusions This study suggested that there is no overlap between the resistance mechanism of co-resistant ECC strains to carbapenem and colistin. However, the emergence of strain co-harboring plasmid-mediated resistance genes indicated that ECC is a potential carrier for the horizontal spread of carbapenems and colistin resistance.

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