scholarly journals Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 79
Author(s):  
Vittoria Mattioni Marchetti ◽  
Ibrahim Bitar ◽  
Mario Sarti ◽  
Elena Fogato ◽  
Erika Scaltriti ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Sequencing Technology ◽  
Genomic Features ◽  
Long Reads ◽  
Clinical Resistance ◽  
Genomic Characterization ◽  
Enterobacteriaceae Infections ◽  
Enterobacter Cloacae Complex ◽  
Conjugation Experiment

Background: the co-production of carbapenemases and mcr-genes represents a worrisome event in the treatment of Enterobacteriaceae infections. The aim of the study was to characterize the genomic features of two clinical Enterobacter cloacae complex (ECC) isolates, co-producing VIM and MCR enzymes, in Italy. Methods: species identification and antibiotic susceptibility profiling were performed using MALDI-TOF and broth microdilution methods, respectively. Transferability of the blaVIM- and mcr- type genes was verified through conjugation experiment. Extracted DNA was sequenced using long reads sequencing technology on the Sequel I platform (PacBio). Results: the first isolate showed clinical resistance against ertapenem yet was colistin susceptible (EUCAST 2020 breakpoints). The mcr-9.2 gene was harbored on a conjugative IncHI2 plasmid, while the blaVIM-1 determinant was harbored on a conjugative IncN plasmid. The second isolate, resistant to both carbapenems and colistin, harbored: mcr-9 gene and its two component regulatory genes for increased expression on the chromosome, mcr-4.3 on non-conjugative (yet co-transferable) ColE plasmid, and blaVIM-1 on a non-conjugative IncA plasmid. Conclusions: to our knowledge, this is the first report of co-production of VIM and MCR in ECC isolates in Italy.

scholarly journals Genomic Study of blaIMI-Positive Enterobacter cloacae Complex in Singapore over a Five-Year Study Period

2020 ◽  
Vol 64 (8) ◽  
Author(s):  
Sophie Octavia ◽  
Tse Hsien Koh ◽  
Oon Tek Ng ◽  
Kalisvar Marimuthu ◽  
Indumathi Venkatachalam ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Mobile Elements ◽  
Content Type ◽  
Genomic Study ◽  
Genomic Characterization ◽  
Enterobacter Cloacae Complex

ABSTRACT The blaIMI gene is rarely detected outside the Enterobacter genus. Genomic characterization of 87 blaIMI-positive Enterobacter cloacae complex members revealed that the largest phylogenomic clade was made up of E. cloacae subsp. cloacae (71.3%), followed by the newly described species E. bugandensis (13.8%), E. sichuanensis (10.3%), and E. roggenkampii (4.6%). IMI-1 was the predominant carbapenemase variant (86/87, 98.9%). All the blaIMI genes were associated with chromosomally integrated Xer-dependent integrative mobile elements (IMEXs), with two new variants detected.

Characterization of NDM-1- and CMH-3-producing Enterobacter cloacae complex ST932 in a patient transferred from Ukraine to Spain

2020 ◽  
Vol 38 (7) ◽  
pp. 327-330
Author(s):  
Inmaculada López-Hernández ◽  
Aurora García Barrionuevo ◽  
Paula Díaz de Alba ◽  
Encarnación Clavijo ◽  
Alvaro Pascual
Keyword(s):  
Enterobacter Cloacae ◽  
Enterobacter Cloacae Complex

scholarly journals The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics

2021 ◽  
Vol 12 ◽  
Author(s):  
Lina Reslan ◽  
Marc Finianos ◽  
Ibrahim Bitar ◽  
Mohamad Bahij Moumneh ◽  
George F. Araj ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Antimicrobial Resistance ◽  
Tetracycline Resistance ◽  
Virulence Gene ◽  
Surveillance Program ◽  
Virulence Determinants ◽  
Tetracycline Resistance Genes ◽  
Long Reads ◽  
Genomic Characterization

BackgroundInvasive pneumococcal disease (IPD) remains a global health problem. IPD incidence has significantly decreased by the use of pneumococcal conjugate vaccines (PCV). Nevertheless, non-PCV serotypes remain a matter of concern. Eight Streptococcus pneumoniae serotype 24F isolates, belonging to a non-PCV serotype, were detected through the Lebanese Inter-Hospital Pneumococcal Surveillance Program. The aim of the study is to characterize phenotypic and genomic features of the 24F isolates in Lebanon.MethodsWGS using long reads sequencing (PacBio) was performed to produce complete circular genomes and to determine clonality, antimicrobial resistance and virulence determinants.ResultsThe sequencing results yielded eight closed circular genomes. Three multilocus sequence typing (MLST) types were identified (ST11618, ST14184, ST15253). Both MLST and WGS analyses revealed that these isolates from Lebanon were genetically homogenous belonging to clonal complex CC230 and clustered closely with isolates originating from Canada, United States of America, United Kingdom and Iceland. Their penicillin binding protein profiles correlated with both β-lactam susceptibility patterns and MLST types. Moreover, the isolates harbored the macrolide and tetracycline resistance genes and showed a similar virulence gene profile. To our knowledge, this study represents the first report of complete phenotypic and genomic characterization of the emerging Streptococcus pneumoniae, serotype 24F, in the Middle East and North Africa region.

scholarly journals Genomic Characterization of Enterobacter cloacae Isolates from China That Coproduce KPC-3 and NDM-1 Carbapenemases

2016 ◽  
Vol 60 (4) ◽  
pp. 2519-2523 ◽  
Author(s):  
Hong Du ◽  
Liang Chen ◽  
Kalyan D. Chavda ◽  
Ruchi Pandey ◽  
Haifang Zhang ◽  
...  
Keyword(s):  
Direct Repeat ◽  
Enterobacter Cloacae ◽  
Tertiary Hospital ◽  
Sequence Type ◽  
Class I ◽  
Content Type ◽  
Repeat Sequences ◽  
Genomic Characterization

ABSTRACTHere, we report twoEnterobacter cloacaesequence type 231 isolates coproducing KPC-3 and NDM-1 that have caused lethal infections in a tertiary hospital in China. TheblaNDM-1-harboring plasmids carry IncA/C2and IncR replicons, showing a mosaic plasmid structure, and theblaNDM-1is harbored on a novel class I integron-like element.blaKPC-3is located on a Tn3-ΔblaTEM-1-blaKPC-3-ΔTn1722element, flanked by two 9-bp direct-repeat sequences and harbored on an IncX6 plasmid.

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