Cluster Analysis and Genetic Characterization of Enterobacter cloacae Complex from Blood Cultures

The knowledge of genetic diversity degree of given species is of great importance for the successful process of breeding and genetic conservation. The aim of conducted research was to determine the genetic differentiation of Norway spruce (Picea abies (L.) Karst) genotypes with very specific narrow pyramidal and normal crown type, which grows at different altitude of the mountain Golija. For assessment of genetic similarities or differences between studied genotypes co-dominant microsatellite system had been used. This system has proven to be reliable and efficient in the genetic characterization of plant species. In total 22 primer sets have been tested, while 16 (73%) of them resulted in the successful yield of the amplified product. The analysis show that studied individuals had in total 130 alleles, in average 8.125 polymorphic alleles per each locus. The lowest polymorphism was detected in the locus EATC1D10, EATC1F03B and EATC2G09, while the highest level of polymorphism was detected in EATC2G08. Based on microsatellite date and similarity matrix, cluster analysis dendrogram indicates existence of the vertical differentiation of studied genotypes, which is consistent with results of previous Norway spruce studies.

Download Full-text

Genomic Study of blaIMI-Positive Enterobacter cloacae Complex in Singapore over a Five-Year Study Period

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00510-20 ◽

2020 ◽

Vol 64 (8) ◽

Author(s):

Sophie Octavia ◽

Tse Hsien Koh ◽

Oon Tek Ng ◽

Kalisvar Marimuthu ◽

Indumathi Venkatachalam ◽

...

Keyword(s):

Enterobacter Cloacae ◽

Mobile Elements ◽

Content Type ◽

Genomic Study ◽

Genomic Characterization ◽

Enterobacter Cloacae Complex

ABSTRACT The blaIMI gene is rarely detected outside the Enterobacter genus. Genomic characterization of 87 blaIMI-positive Enterobacter cloacae complex members revealed that the largest phylogenomic clade was made up of E. cloacae subsp. cloacae (71.3%), followed by the newly described species E. bugandensis (13.8%), E. sichuanensis (10.3%), and E. roggenkampii (4.6%). IMI-1 was the predominant carbapenemase variant (86/87, 98.9%). All the blaIMI genes were associated with chromosomally integrated Xer-dependent integrative mobile elements (IMEXs), with two new variants detected.

Download Full-text

Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China

BMC Infectious Diseases ◽

10.1186/s12879-020-4768-7 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Yongxin Zhao ◽

Jisheng Zhang ◽

Yanjun Fu ◽

Chunjiang Li ◽

Kewang Hu ◽

...

Keyword(s):

Molecular Characterization ◽

Enterobacter Cloacae ◽

Heilongjiang Province ◽

Carbapenem Resistant ◽

Enterobacter Cloacae Complex

Download Full-text

Characterization of Multiple NDM-1-Producing Enterobacteriaceae Isolates from the Same Patient

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.04862-14 ◽

2015 ◽

Vol 59 (6) ◽

pp. 3648-3651 ◽

Cited By ~ 21

Author(s):

Nathalie Tijet ◽

David Richardson ◽

Gregory MacMullin ◽

Samir N. Patel ◽

Roberto G. Melano

Keyword(s):

Escherichia Coli ◽

Male Patient ◽

Community Hospital ◽

Enterobacter Cloacae ◽

Blood Cultures ◽

Content Type ◽

Carbapenem Resistant ◽

Plasmid Analysis ◽

Providencia Stuartii

ABSTRACTA male patient was admitted to a community hospital in Ontario, Canada, with an infected sacral ulcer after returning from India, where he was hospitalized. Carbapenem-resistantEscherichia coli(isolated from blood cultures),Enterobacter cloacae, andProvidencia stuartii(from urine samples), all positive forblaNDM-1, were recovered. Comparative NDM-1 plasmid analysis suggests both lateral plasmid transfer and independent acquisition of theblaNDM-1gene in these clinical isolates.

Download Full-text

Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy

Diagnostics ◽

10.3390/diagnostics11010079 ◽

2021 ◽

Vol 11 (1) ◽

pp. 79

Author(s):

Vittoria Mattioni Marchetti ◽

Ibrahim Bitar ◽

Mario Sarti ◽

Elena Fogato ◽

Erika Scaltriti ◽

...

Keyword(s):

Enterobacter Cloacae ◽

Sequencing Technology ◽

Genomic Features ◽

Long Reads ◽

Clinical Resistance ◽

Genomic Characterization ◽

Enterobacteriaceae Infections ◽

Enterobacter Cloacae Complex ◽

Conjugation Experiment

Background: the co-production of carbapenemases and mcr-genes represents a worrisome event in the treatment of Enterobacteriaceae infections. The aim of the study was to characterize the genomic features of two clinical Enterobacter cloacae complex (ECC) isolates, co-producing VIM and MCR enzymes, in Italy. Methods: species identification and antibiotic susceptibility profiling were performed using MALDI-TOF and broth microdilution methods, respectively. Transferability of the blaVIM- and mcr- type genes was verified through conjugation experiment. Extracted DNA was sequenced using long reads sequencing technology on the Sequel I platform (PacBio). Results: the first isolate showed clinical resistance against ertapenem yet was colistin susceptible (EUCAST 2020 breakpoints). The mcr-9.2 gene was harbored on a conjugative IncHI2 plasmid, while the blaVIM-1 determinant was harbored on a conjugative IncN plasmid. The second isolate, resistant to both carbapenems and colistin, harbored: mcr-9 gene and its two component regulatory genes for increased expression on the chromosome, mcr-4.3 on non-conjugative (yet co-transferable) ColE plasmid, and blaVIM-1 on a non-conjugative IncA plasmid. Conclusions: to our knowledge, this is the first report of co-production of VIM and MCR in ECC isolates in Italy.

Download Full-text

Molecular Characterization of KPC-2-Producing Enterobacter cloacae Complex Isolates from Cali, Colombia

Antibiotics ◽

10.3390/antibiotics10060694 ◽

2021 ◽

Vol 10 (6) ◽

pp. 694

Author(s):

Aura Falco ◽

Daniela Guerrero ◽

Isabella García ◽

Adriana Correa ◽

Sandra Rivera ◽

...

Keyword(s):

Molecular Characterization ◽

Public Health Problem ◽

Carbapenem Resistance ◽

Enterobacter Cloacae ◽

Epidemiological Surveillance ◽

Beta Lactam ◽

High Genetic Diversity ◽

Effective Prevention ◽

Enterobacter Cloacae Complex

The Enterobacter cloacae complex is an emerging opportunistic pathogen whose increased resistance to carbapenems is considered a public health problem. This is due to the loss of efficacy of beta-lactam antibiotics, which are used as the first treatment option in the management of infections caused by Gram-negative bacteria. The objective of this study was to perform the molecular characterization of 28 isolates of the E. cloacae complex resistant to cephalosporins and carbapenems isolated between 2011 and 2018 from five hospitals located in the municipality of Santiago de Cali, Colombia. Molecular detection of blaKPC, blaVIM, blaNDM and blaOXA-48-like genes was performed on these isolates and the genetic relationship between the isolates was assessed using multilocus sequence typing (MLST). Forty-three percent of the isolates carried the blaKPC-2 gene variant. MLST showed high genetic diversity among isolates, the most frequent being the sequence type ST510 with a frequency of 50%. The identification of the genes involved in carbapenem resistance and dispersing genotypes is an important step toward the development of effective prevention and epidemiological surveillance strategies in Colombian hospitals.

Download Full-text