scholarly journals Proteomic analysis of latex from Jatropha curcas L. stems and comparison of two classic proteomic sample isolation methods: The phenol extraction and the TCA/acetone extraction

2017 ◽  
Vol 27 ◽  
pp. 14-24 ◽  
Author(s):  
Song Yang ◽  
Meng-Meng Ding ◽  
Fang Chen ◽  
Ying Xu
2015 ◽  
Vol 14 (6) ◽  
pp. 2557-2568 ◽  
Author(s):  
Mohibullah Shah ◽  
Emanoella L. Soares ◽  
Paulo C. Carvalho ◽  
Arlete A. Soares ◽  
Gilberto B. Domont ◽  
...  

2017 ◽  
Vol 5 (2) ◽  
pp. 251-263
Author(s):  
Indah Retnowati ◽  
Memen Surahman

Indonesia memiliki banyak  tanah masam, tetapi penggunaan masih sangat sedikit karena kandungan nutrisi tanah masam rendah. Sementara itu, jarak pagar merupakan tanaman yang dapat tumbuh di berbagai jenis tanah (termasuk lahan marjinal) dan penggunaannya sebagai bahan baku biodiesel. Oleh karena itu, perlunya penelitian mengenai pertumbuhan jarak pagar pada tanah masam sebagai upaya untuk mengembangkan jarak pagar dan penggunaannya di Indonesia.Untuk memulai upaya, penelitian tentang berbagai genotipe jarak pagar di tanah masam telah dilakukan. Tujuan dari penelitian ini yaitu mempelajari genotipe jarak pagar  yang berpotensi untuk tanah masam. Penelitian ini dilakukan di UPTD Pengembangan Teknologi Lahan Kering Singabraja, Kecamatan Tenjo, Bogor, Jawa Barat, dari November 2010-Agustus 201. Penelitian ini menggunakan Rancangan Kelompok Lengkap Teracak (RKLT) dengan satu faktor yaitu genotipe. Hasil penelitian menunjukkan berbagai perbedaan pada pertumbuhan diantara genotipe-genotipe jarak pagar. Secara umum, ada lima genotipe jarak pagar yang terbaik di tanah masam (pH 5.0) yaitu Medan I-5-1, Dompu, IP-2P-3-4-1, Sulawesi, dan Bima M.


2016 ◽  
Vol 34 (12) ◽  
pp. 1131
Author(s):  
Yejing WENG ◽  
Zhigang SUI ◽  
Lihua ZHANG ◽  
Yukui ZHANG

2018 ◽  
Vol 10 (5) ◽  
pp. 1451 ◽  
Author(s):  
Mario Giraldi-Díaz ◽  
Lorena De Medina-Salas ◽  
Eduardo Castillo-González ◽  
Max De la Cruz-Benavides

2017 ◽  
Vol 8 ◽  
Author(s):  
Haiyan Li ◽  
Suguru Tsuchimoto ◽  
Kyuya Harada ◽  
Masanori Yamasaki ◽  
Hiroe Sakai ◽  
...  

Separations ◽  
2021 ◽  
Vol 8 (4) ◽  
pp. 53
Author(s):  
Clarissa Braccia ◽  
Bhakti Prinsi ◽  
Mara Colzani ◽  
Alessandra A. Altomare ◽  
Luca Espen ◽  
...  

The benefits of ginseng have been mainly attributed to its triterpenoids, called ginsenosides. Recent genome sequencing of the Panax ginseng has paved the way for in-depth proteomic studies of this medicinal plant. The current study was conducted to deepen the proteomic information on the root proteome of Korean ginseng. Proteomic workflow was optimized by testing two different strategies, characterized by the phenol extraction procedure, the presence or the absence of SDS-PAGE fractionation step, and nano-scale liquid chromatographic tandem mass spectrometry (nLC-MS/MS) analysis. The results highlighted an evident improvement of proteome extraction by the combination of phenol extraction with SDS-PAGE before the nLC-MS/MS analysis. In addition, a dramatic impact of the steaming process (the treatment to produce red ginseng from ginseng) on protein properties was observed. Overall, the analyses of Korean ginseng permitted the characterization of a total of 2412 proteins. A large number of identified proteins belonged to the functional categories of protein and carbon/energy metabolism (22.4% and 14.6%, respectively). The primary and secondary metabolisms are major metabolic pathways, which emerged from the proteomic analysis. In addition, a large number of proteins known to play an important role in response to (a)biotic stresses were also identified. The current proteomic study not only confirmed the previous transcriptomic and proteomic reports but also extended proteomic information, including the main metabolic pathways involved in Korean ginseng.


2020 ◽  
Vol 151 ◽  
pp. 112482
Author(s):  
N.H.C. Mat ◽  
M.A.R. Bhuiyan ◽  
Z. Yaakob ◽  
R. Wickneswari

2016 ◽  
Vol 149 ◽  
pp. 431-437 ◽  
Author(s):  
A. Ruggiero ◽  
R. D’Amato ◽  
M. Merola ◽  
P. Valášek ◽  
M. Müller

2012 ◽  
Vol 113 (2) ◽  
pp. 437-442 ◽  
Author(s):  
Erisandro S. Silva ◽  
Marta M. Conceição ◽  
Eduardo H. S. Cavalcanti ◽  
Valter J. Fernandes ◽  
Ana C. D. Medeiros ◽  
...  

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