scholarly journals Effect of ultraviolet radiation on physiological and biochemical properties of yeast Saccharomyces cerevisiae during fermentation of ultradispersed starch raw material

2018 ◽  
Vol 31 ◽  
pp. 61-66 ◽  
Author(s):  
Victor Revin ◽  
Nelli Atykyan ◽  
Ekaterina Lyovina ◽  
Yuliya Dragunova ◽  
Victoriya Ushkina
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ultraviolet Radiation ◽  
Biochemical Properties ◽  
Raw Material ◽  
Yeast Saccharomyces Cerevisiae ◽  
Physiological And Biochemical ◽  
Physiological And Biochemical Properties

The physiological and biochemical properties regulation of lager brewer's yeast

2021 ◽  
pp. 52-55
Author(s):  
Марина Васильевна Гернет ◽  
Ольга Алексеевна Борисенко ◽  
Ирина Николаевна Грибкова
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Biomass Accumulation ◽  
Biochemical Properties ◽  
Specific Rate ◽  
Yeast Saccharomyces Cerevisiae ◽  
Lager Yeast ◽  
Yeast Biomass ◽  
Original Culture ◽  
Physiological And Biochemical ◽  
Physiological And Biochemical Properties

Современные методы интенсификации производства напитков брожения часто основаны на активации жизнедеятельности дрожжей под воздействием различных технологических факторов. Одним из путей интенсификации является применение повышенной температуры в процессе накопления биомассы посевных дрожжей, что значительно влияет на удельную скорость размножения микроорганизмов. Исследование направлено на изучение влияния температурного режима накопления биомассы на морфологические и биохимические признаки дрожжей низового брожения. Изучение поставленной задачи проводилось с применением дрожжей низового брожения Saccharomyces cerevisiae расы 8аМ, f, 44 при трех температурных режимах: (6±2) °С, (20±2) °С и (30±2) °С. Исследования показали, что бродильная активность всех исследуемых рас дрожжей, биомасса которых накапливалась при (6±2) °С, выше, чем при (20±2) °С и при более (30±2) °С. Установлено, что в процессе накопления биомассы дрожжей S. cerevisiae расы 44 при температуре (30±2) °С происходит вытеснение исходной культуры другими по форме и размерам клеток. Выявлено пять отличных от исходной культуры колоний, изменения в которых устойчиво сохранялись при последующих многократных пересевах. Пять полученных штаммов обладали более низкой бродильной активностью по сравнению с исходной культурой, а три штамма полностью утратили флокуляционную способность. Modern methods of intensifying the fermentation beverages production are often based on the yeast vital activity activation under the various technological factors influence. One of the ways of intensification is the high temperature use in the process of seed yeast biomass accumulation, which significantly affects the specific rate of microorganisms reproduction. The study is aimed at studying the biomass accumulation temperature regime influence on the lager yeast morphological and biochemical characteristics. The task study was carried out with the use of lager yeast Saccharomyces cerevisiae race 8aM, f, 44 at three temperature conditions: (6±2) °C, (20±2) °C and (30±2) °C. Studies have shown that the fermentation activity of all the studied yeast races, the biomass of which was accumulated at (6±2) °C, is higher than at (20±2) °C and at more than (30±2) °C. It was found that during the S. cerevisiae race 44 yeast biomass accumulation at a temperature of (30±2) °C, the original culture is replaced by other cells in shape and size. Five colonies different from the original culture were identified, the changes in which were steadily preserved during subsequent repeated replanting. Five of the resulting strains had a lower fermentation activity compared to the original culture, and three strains completely lost their flocculation ability.

scholarly journals Fermentación de la almendra de copoazú (Theobroma grandiflorum [Willd. ex Spreng.] Schum.): evaluación y optimización del proceso

2010 ◽  
Vol 11 (2) ◽  
pp. 107 ◽  
Author(s):  
Jenifer Criollo ◽  
Dagoberto Criollo ◽  
Angélica Sandoval Aldana
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Process Optimization ◽  
Fermentation Process ◽  
Raw Material ◽  
Sensory Panel ◽  
Yeast Saccharomyces Cerevisiae ◽  
Theobroma Grandiflorum ◽  
Cutting Test ◽  
Maximum Range ◽  
Low Levels

<p>La almendra de copoazú como producto promisorio para la industria de cosméticos, chocolate, bebidas, licores y conservas, se evaluó el proceso de fermentación variando el tiempo de remoción de la masa (24 y 48 horas) y la pulpa inicial (30 y 100%). Se tuvieron en cuenta las condiciones de los productores en el acceso a equipos de despulpado. Se cuantificó la temperatura de la masa en tres puntos (superior, medio e inferior), acidez, pH, humedad, prueba de corte y análisis sensorial. Se encontró bajo desarrollo de la temperatura de fermentación en los tratamientos con 100% de pulpa y se registraron las máximas temperaturas entre 35 y 36°C que indican deficiencias en el proceso; no se alcanzó los 40°C requeridos para la muerte del embrión. El 30% de pulpa inicial y la remoción cada 24 horas por 9 días, fueron las mejores condiciones encontradas. La optimización con 0,1% de levadura (Saccharomyces cerevisiae) aumentó la temperatura de fermentación hasta 44°C, los granos fermentados hasta 56,14% y el mayor desarrollo de sabores frutales con intensidad de 4, mostrando un mejor proceso de fermentación. El panel sensorial mostró que los licores de copoazú tienen notas frutales destacadas y bajos valores de otros sabores evaluados. Los resultados son semejantes a los cacaos criollos, conocidos en el mundo como materia prima de licores finos y de aroma.</p><p> </p><p><strong>Fermentation of the copoazu kernel (Theobroma grandiflorum [Willd. ex Spreng.] Schum.): Assessmente and process optimization</strong></p><p>The fermentation of copoazu kernels (a promising product for the cosmetics industry, chocolate, beverages, liquors and preserves) was evaluated varying the time of mass removal (24 and 48 hours) and the initial pulp (30 and 100%). This study took into account the degree of access the producers had to pulping equipment. We quantified temperature of the mass at three points (top, middle and bottom), acidity, pH, moisture, cutting test and sensory analysis. The observed temperatures during fermentation in the treatments with 100% pulp reached a maximum range between 35 and 36°C which indicated deficiencies in the process as the 40°C required for the death of the seed was not attained. Thirty percent initial pulp with removal every 24 hours for 9 days yielded the best results. Optimization with 0.1% yeast (Saccharomyces cerevisiae) increased the fermentation temperature to 44°C, augmented fermented beans to 56.14% and saw a development of fruit flavors with an intensity of 4, demonstrating a better fermentation process. The sensory panel showed that copoazu liquors have outstanding fruity notes and low levels of other evaluated flavors. The results are similar to the criollo cacao, known worldwide as a raw material for fine liquors and fragrances.</p>

scholarly journals Active Antibiotics Production by Actinomycetes Indigenous To Saudi Arabia Soils

2021 ◽  
Vol 11 (6) ◽  
pp. 20-29
Author(s):  
Fetoon M ◽  
helaiwi Alk ◽  
Ismet Ara ◽  
Nadine Moubayed
Keyword(s):  
Saudi Arabia ◽  
Biochemical Properties ◽  
Soil Samples ◽  
Fungal Strain ◽  
Pathogenic Microorganisms ◽  
Bacterial Strains ◽  
Desert Soils ◽  
Growth Environment ◽  
Physiological And Biochemical ◽  
Physiological And Biochemical Properties

Streptomyces are the most popular among the Actinomycetes groups and found in soils worldwide. They form an important part of the soil ecology within the Actinomycetales order. Streptomyces are diverse as secondary antibiotic metabolites such as Novobiocin, Amphotericin, Vancomycin, Neomycin, Gentamicin, Chloramphenicol, Tetracycline, Erythromycin and Nystatin. Thus, the current study was aimed to isolate, identify and assess the active antibiotic metabolites produced by different actinomyces sp. found in Saudi Arabian soils. Six samples were collected from desert soils of the Al Thumamah area and analyzed using GS-MS. Scanning Electron Microscopy was used to identify the bacterial strains along with their antibiotic metabolites effectiveness of secondary metabolites (antibiotics) against different Gram-positive (Bacillus subtilis, Staphylococcus aureus), negative pathogens (Pseudomonas aeruginosa, Escherichia coli, Salmonella suis, and Shigella sonnei) as well as the fungal strain Candida albicans was investigated. Thirty active bacterial (F1-30) strains were isolated from the soil samples and the strains F3, F7, F22, F30 have white, gray, pink, yellow and red colours respectively. Only ten strains (F13, F14, F15, F16, FI7, F18, F19, F20, F21, and F22) were found to have antimicrobial activity against at least one pathogen. The optimum growth environment was pH 4-10, temperature (300C), and NaCl (7% w/v) concentration. According to our findings, the extreme desert environment of Al Thumamah from Saudi Arabia is rich in its actinobacterial population with diverse colouring groups and various physiological and biochemical properties. This shows it’s capability of generating secondary metabolite elements that could inhibit pathogenic microorganisms.

scholarly journals Physiology and Nutrition of Treponema primitia, an H2/ CO2-Acetogenic Spirochete from Termite Hindguts

2004 ◽  
Vol 70 (3) ◽  
pp. 1307-1314 ◽  
Author(s):  
Joseph R. Graber ◽  
John A. Breznak
Keyword(s):  
Nadh Oxidase ◽  
Natural Habitat ◽  
Biochemical Properties ◽  
Organic Substrates ◽  
Growth And Survival ◽  
Cell Extracts ◽  
Termite Hindgut ◽  
Physiological And Biochemical ◽  
Physiological And Biochemical Properties

ABSTRACT Treponema primitia strains ZAS-1 and ZAS-2, the first spirochetes to be isolated from termite hindguts (J. R. Leadbetter, T. M. Schmidt, J. R. Graber, and J. A. Breznak, Science 283:686-689, 1999), were examined for nutritional, physiological, and biochemical properties relevant to growth and survival in their natural habitat. In addition to using H2 plus CO2 as substrates, these strains were capable of homoacetogenic growth on mono- and disaccharides and (in the case of ZAS-2) methoxylated benzenoids. Cells were also capable of mixotrophic growth (i.e., simultaneous utilization of H2 and organic substrates). Cell extracts of T. primitia possessed enzyme activities of the Wood/Ljungdahl (acetyl coenzyme A) pathway of acetogenesis, including tetrahydrofolate-dependent enzymes of the methyl group-forming branch. However, a folate compound was required in the medium for growth. ZAS-1 and ZAS-2 growing on H2 plus CO2 displayed H2 thresholds of 650 and 490 ppmv, respectively. Anoxic cultures of ZAS-1 and ZAS-2 maintained growth after the addition of as much as 0.5% (vol/vol) O2 to the headspace atmosphere. Cell extracts exhibited NADH and NADPH peroxidase and NADH oxidase activities but neither catalase nor superoxide dismutase activity. Results indicate that (i) T. primitia is able to exploit a variety of substrates derived from the food of its termite hosts and in so doing contributes to termite nutrition via acetogenesis, (ii) in situ growth of T. primitia is likely dependent on secretion of a folate compound(s) by other members of the gut microbiota, and (iii) cells possess enzymatic adaptations to oxidative stress, which is likely to be encountered in peripheral regions of the termite hindgut.

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