A primary cell culture of Drosophila postembryonic larval neuroblasts to study cell cycle and asymmetric division

2006 ◽  
Vol 85 (6) ◽  
pp. 567-575 ◽  
Author(s):  
Julian Ceron ◽  
Francisco J. Tejedor ◽  
Fernando Moya
Keyword(s):  
Cell Cycle ◽  
Cell Culture ◽  
Primary Cell Culture ◽  
Asymmetric Division ◽  
Primary Cell

Preclinical evidence of ET-743 as a potential chemotherapy option for the treatment of biliary carcinoma.

2013 ◽  
Vol 31 (4_suppl) ◽  
pp. 193-193
Author(s):  
Francesco Leone ◽  
Caterina Peraldo-Neia ◽  
Giuliana Cavalloni ◽  
Marco Soster ◽  
Loretta Gammaitoni ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Culture ◽  
Dna Damage ◽  
Clinical Trials ◽  
Cell Lines ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
Term Survival ◽  
Anti Tumor Activity

193 Background: The standard chemotherapy for unresectablebiliary tract carcinoma (BTC) is based on gemcitabine and platinum compounds. However, these combinations have not been shown to be effective in improving long-term survival. Thus, there is a real need to find new strategies that would impact in a significant way on clinical outcome. Ecteinascidin-743 (ET-743), a compound isolated from the marine tunicate Ecteinascidia turbinata. ET-743, is approved for the treatment of ovarian cancer and soft tissue sarcoma. Phase II and III clinical trials are ongoing for the treatment of different solid tumors. No preclinical data are available about the efficacy of ET-743 in BTC. In a phase I study, one patient received ET-743 plus capecitabine and experienced a long lasting complete metabolic response. Here, we investigated the antitumor activity of ET-743 in preclinical BTC models. Methods: Four BTC cell lines TFK1, EGI-1, HuH28 and TGBC1 were used to evaluate the effect of ET-743 on proliferation, cell cycle, apoptosis and on the activation of DNA damage proteins. The effect on proliferation was also investigated on a primary cell culture of a gallbladder carcinoma (GBC) resistant to gemcitabine and oxaliplatin. On the same cells, the inhibition of VEGF secretion mediated by ET-743 was analyzed by ELISA. The anti-tumor activity of ET-743 was tested on EGI-1 xenografts in NOD/SCID mice. Results: In vitro, ET-743 is able to markedly reduce cell proliferation of BTC cell lines through cell cycle blockage on G0/G1 phase and to inhibit the growth of primary cell culture derived from GBC patient. Moreover, ET-743 promotes apoptosis by caspase 3 activation, activates proteins involved in DNA damage and reduces VEGF secretion. In the in vivo model, ET-743 is able to slow tumor growth in BTC xenograft. The mechanism of anti-tumor activity involves DNA damage, the induction of hypoxia transcription factor-1, and angiogenesis inhibition. ET-743 has no significant effect on apoptosis in vivo. Conclusions: These data suggest that ET-743 could represent an alternative chemotherapy for BTC treatment and encourage the development of clinical trials of ET-743 in BTC patients.

The role of ER stress for the pathogenesis of SCA3 in primary cell culture experiment

2007 ◽  
Vol 34 (S 2) ◽  
Author(s):  
C Funke ◽  
J Hübener ◽  
H Wolburg ◽  
T Schmidt ◽  
H Toresson ◽  
...  
Keyword(s):  
Cell Culture ◽  
Er Stress ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
Culture Experiment ◽  
Cell Culture Experiment

scholarly journals Gene Expression Profiling of Extracellular Matrix as an Effector of Human Hepatocyte Phenotype in Primary Cell Culture

2007 ◽  
Vol 97 (2) ◽  
pp. 384-397 ◽  
Author(s):  
J. L. Page ◽  
M. C. Johnson ◽  
K. M. Olsavsky ◽  
S. C. Strom ◽  
H. Zarbl ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Primary Cell Culture ◽  
Human Hepatocyte ◽  
Primary Cell

Primary Cell Culture

1994 ◽  
pp. 113-136 ◽  
Author(s):  
Bernice M. Martin
Keyword(s):  
Cell Culture ◽  
Primary Cell Culture ◽  
Primary Cell

Primary cell culture from embryos of the Japanese scallop Mizuchopecten yessoensis (Bivalvia)

1991 ◽  
Vol 6 (1) ◽  
pp. 49-54 ◽  
Author(s):  
N. A. Odintsova ◽  
A. V. Khomenko
Keyword(s):  
Cell Culture ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
Japanese Scallop

A magnetic three‐dimensional levitated primary cell culture system for the development of secretory salivary gland‐like organoids

2019 ◽  
Vol 13 (3) ◽  
pp. 495-508 ◽  
Author(s):  
Joao N. Ferreira ◽  
Riasat Hasan ◽  
Ganokon Urkasemsin ◽  
Kiaw K. Ng ◽  
Christabella Adine ◽  
...  
Keyword(s):  
Cell Culture ◽  
Salivary Gland ◽  
Culture System ◽  
Primary Cell Culture ◽  
Three Dimensional ◽  
Primary Cell ◽  
Cell Culture System

scholarly journals Novel methods for in vitro modeling of pancreatic cancer reveal important aspects for successful primary cell culture

BMC Cancer ◽  
2020 ◽  
Vol 20 (1) ◽  
Author(s):  
L. Ehlen ◽  
J. Arndt ◽  
D. Treue ◽  
P. Bischoff ◽  
F. N. Loch ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cell Culture ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
In Vitro Modeling ◽  
Novel Methods
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