The research was conducted on three small islands of marine conservation area of West Sumatra, namely Kasiak Island, Bindalang Island and Karabak Ketek Island from January to December 2016. This research aimed to study the variation of biophysical character of nesting habitat of green turtle (Chelonia mydas L, 1758) by survey method, measurement, observation and analysis. All data were analyzed using Main Component Analysis (PCA), Kriskal Wallis Test Analysis. Based on PCA analysis, the contribution on main character is 43.28%. These results are supported by biophysical conditions of spawning habitats for green turtles is on Karabak Ketek Island as an ideal island of spawning sites favored by green turtles, when compared to the location of Bindalang Island and Kasiak Island habitats.Result of Kruscal Wallis analysis of Karabak Island rank; 11.90, with a chi-square value; 10.47, asymp sig value 0.005 (5% -10%). This value shows the difference between the biophysical character of the spawning habitat on the character of the coastal slope parameter (KP) is 9.60 °.This is also the ideal slope value for the sandy beach habitat (PSe) and fine sandy beaches (PHA) as the main spawning location for green turtles in West Sumatra