The impact of One-Step Nucleic Acid Amplification (OSNA) on operative efficiency.

2014 ◽  
Vol 40 (5) ◽  
pp. 654
Author(s):  
Melissa Ley-Hui Tan ◽  
Lay In Lim ◽  
Ghaleb Goussous ◽  
Sankaran Narayanan ◽  
Robert Kirby ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
One Step ◽  
The Impact

scholarly journals Association of One-Step Nucleic Acid Amplification Detected Micrometastases with Tumour Biology and Adjuvant Chemotherapy

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Ghaleb Goussous ◽  
Sadaf Jafferbhoy ◽  
Niamh Smyth ◽  
Lisette Hammond ◽  
Sankaran Narayanan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Adjuvant Chemotherapy ◽  
Sentinel Node ◽  
Survival Benefit ◽  
Nucleic Acid Amplification ◽  
Detection Rates ◽  
Tumour Biology ◽  
Number Of Patients ◽  
One Step ◽  
The Impact

One-step nucleic acid amplification (OSNA) is an intraoperative technique with a high sensitivity and specificity for sentinel node assessment. The aim of this study was to assess the impact of OSNA on micrometastases detection rates and use of adjuvant chemotherapy. A retrospective review of patients with sentinel node micrometastases over a five-year period was carried out and a comparison of micrometastases detection using OSNA and H&E techniques was made. Out of 1285 patients who underwent sentinel node (SLN) biopsy, 76 patients had micrometastases. Using H&E staining, 36 patients were detected with SLN micrometastases (9/year) in contrast to 40 patients in the OSNA year (40/year) (p<0.0001), demonstrating a fourfold increase with the use of OSNA. In the OSNA group, there was also a proportional increase in Grade III, triple-negative, ER-negative, and HER-2-positive tumours being diagnosed with micrometastases. Also on interactive PREDICT tool, the number of patients with a predicted 10-year survival benefit of more than 3% with adjuvant chemotherapy increased from 52 to 70 percent. OSNA has resulted in an increased detection rate of micrometastases especially in patients with aggressive tumour biology. This increased the number of patients who had a predicted survival benefit from adjuvant chemotherapy.

The impact of One-Step Nucleic Acid Amplification (OSNA) on the identification and management of micrometastases in the axilla

2014 ◽  
Vol 40 (5) ◽  
pp. 639
Author(s):  
Ghaleb Goussous ◽  
Niamh Smyth ◽  
Lay-In Lim ◽  
Melissa Lay-Hui Tan ◽  
Sankaran Narayanan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
One Step ◽  
The Impact

scholarly journals Ultrastaging Using Ex Vivo Sentinel Lymph Node Mapping and One-Step Nucleic Acid Amplification (OSNA) in Gastric Cancer: Experiences of a European Center

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2683
Author(s):  
Bruno Märkl ◽  
Bianca Grosser ◽  
Kerstin Bauer ◽  
Dmytro Vlasenko ◽  
Gerhard Schenkirsch ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Detection Rate ◽  
Ex Vivo ◽  
Sentinel Lymph Node Mapping ◽  
Nucleic Acid Amplification ◽  
Lymph Node Mapping ◽  
Single Positive ◽  
Conventional Histology ◽  
One Step ◽  
Sensitivity Specificity

Background: In this study, the effectiveness of One-step nucleic acid amplification (OSNA) in combination with ex vivo SLN mapping is compared with conventional histology including immunohistochemistry. Methods: LNs were retrieved from gastrectomy specimens in an unfixed state. After ex vivo SLN mapping using methylene-blue, LNs were sliced to provide samples for histology and OSNA. Results: In total, 334 LNs were retrieved in the fresh state from 41 patients. SLN detection was intended in 40 cases but was successful in only 29, with a correct LN status prediction in 23 cases (79%). Excluding one case out of 41 with a failure likely caused by a processing error, OSNA showed a high effectiveness with sensitivity, specificity, and accuracy rates of 85.4%, 93.5%, and 92.4%, respectively. The LN status could be predicted in all but one case, in which the single positive LN was not eligible for OSNA testing. Moreover, OSNA evaluation led to upstaging from N0 to N+ in three cases (14%). Conclusion: The ex vivo SLN protocol used resulted in a relatively poor detection rate. However, the OSNA method was not hampered by this detection rate and proved its potential to increase the sensitivity of metastases detection.

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