Continuous sweat lactate monitoring system with integrated screen-printed Mgo-templated carbon-lactate oxidase biosensor and microfluidic sweat collector

2021 ◽  
Vol 368 ◽  
pp. 137620
Author(s):  
Isao Shitanda ◽  
Masaya Mitsumoto ◽  
Noya Loew ◽  
Yuko Yoshihara ◽  
Hikari Watanabe ◽  
...  
Keyword(s):  
Monitoring System ◽  
Lactate Oxidase ◽  
Sweat Lactate ◽  
Templated Carbon ◽  
Screen Printed

Continuous Sweat Lactate Sensor Based on Screen-Printed MgO-Templated Carbon with Integrated Flow System

2020 ◽  
Vol MA2020-02 (44) ◽  
pp. 2799-2799
Author(s):  
Noya Loew ◽  
Yuko Yoshihara ◽  
Masaya Mitsumoto ◽  
Yoshinao Hoshi ◽  
Hikari Watanabe ◽  
...  
Keyword(s):  
Flow System ◽  
Lactate Sensor ◽  
Sweat Lactate ◽  
Templated Carbon ◽  
Screen Printed

A Wearable Biosensor Based on Bienzyme Gel-Membrane for Sweat Lactate Monitoring by Mounting on Eyeglasses

2020 ◽  
Vol 20 (3) ◽  
pp. 1495-1503 ◽  
Author(s):  
Lili Zhang ◽  
Jian Liu ◽  
Zhenling Fu ◽  
Liguo Qi
Keyword(s):  
Physical Exercise ◽  
Low Noise ◽  
Lactate Oxidase ◽  
Electron Mediator ◽  
Enzymatic Biosensor ◽  
Current Change ◽  
Chemical Selectivity ◽  
Sweat Lactate ◽  
Change Tendency

A new enzymatic biosensor worn on eyeglasses has been developed for low-noise and noninvasive determination of lactate in human sweat during physical exercise. The Os (osmium)-complex, the electron mediator between the enzyme and the electrode, was first immobilized on a flexibly printed carbon electrode. Then, a gel membrane with the stereoscopic reticular structure of lactate oxidase and horseradish peroxidase was casted on the electrode to form the biosensor. Linearity of the biosensor was observed for up to 25 mM lactate in a phosphate buffered solution of pH 7.0. Chemical selectivity was evaluated by adding common interferent species such as ascorbic acid, glucose and uric acid to the lactate. The negligible current interference indicated excellent discriminatory selectivity of the biosensor. Applied to an analysis of the real sweat lactate dynamics of healthy subjects during cycling exercise, the amperometric profiles of the biosensors reflected changes in sweat lactate that depended on physical exercise intensity. Compared with other reported epidermal biosensors attached to the arm or leg, our biosensor not only exhibited a similar current change tendency but also rarely suffered from deformational interference due to their forehead measurement position. Such a successful application of real-time monitoring of sweat lactate means that eyeglass-bound biosensors hold considerable promise in the physical exercise and biomedical fields.

Self-Powered Wearable Sweat-Lactate Analyzer for Scheduling Training of Boat Race

2020 ◽  
Vol 15 (2) ◽  
pp. 212-218 ◽  
Author(s):  
Yupeng Mao ◽  
Ning Ba ◽  
Xinchao Gao ◽  
Ziqi Wang ◽  
Mailun Shen ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Piezoelectric Effect ◽  
Physiological State ◽  
Enzymatic Reaction ◽  
Driving Frequency ◽  
Lactate Oxidase ◽  
Training Plan ◽  
Self Powered ◽  
The Relationship ◽  
Sweat Lactate

Self-powered wearable sweat-lactate analyzer has been developed for training analysis of rowing. Tetrapodshaped ZnO nanowires are attached onto ordinary textiles to form a device. Based on the coupling of enzymatic reaction (lactate oxidase and lactic acid) and piezoelectric effect, the device can be attached to the tester to monitor the driving frequency, rowing distance and sweat lactic acid concentration of the tester in real-time. The relationship between the rowing frequency and physiological state is obtained by monitoring the rowing process of the tester, which is helpful to formulate a training plan belonging to the rowing tester. This study provides a new direction for the scientific monitoring of kinematics and accelerates the development of wearable sports equipment.

Screen-Printed Lactate Biosensor Based on a Lox-Immobilized MgO-Templated Carbon for Continuously Monitoring of Lactate Concentration in Sweat

2020 ◽  
Vol MA2020-02 (44) ◽  
pp. 2800-2800
Author(s):  
Masaya Mitsumoto ◽  
Yoshinao Hoshi ◽  
Hikari Watanabe ◽  
Yuko Yoshihara ◽  
Noya Loew ◽  
...  
Keyword(s):  
Lactate Concentration ◽  
Templated Carbon ◽  
Screen Printed

Stabilization of lactate oxidase in screen-printed enzyme electrodes

1996 ◽  
Vol 11 (8) ◽  
pp. 833-837 ◽  
Author(s):  
A.L. Hart ◽  
H. Cox ◽  
D. Janssen
Keyword(s):  
Lactate Oxidase ◽  
Enzyme Electrodes ◽  
Screen Printed

scholarly journals Continuous assessment of sweat lactic acid secretion using microfluidic sweat lactic acid monitoring system

2021 ◽  
Vol 1192 (1) ◽  
pp. 012006
Author(s):  
Y Goto ◽  
Y Suzuki ◽  
K Morisawa ◽  
A Hosoyama ◽  
Y Taira ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Monitoring System ◽  
Continuous Monitoring ◽  
Carbon Electrodes ◽  
Clinical Tests ◽  
Continuous Assessment ◽  
Lactate Oxidase ◽  
Sampling Device ◽  
Non Invasive ◽  
Phosphate Buffered Saline

Abstract A microfluidic sweat lactic acid (LA) monitoring system for non-invasive and continuous assessment of sweat LA was constructed and tested. Our system transports whole secretions from the skin to an electrochemical LA biosensor using a continuous flow of phosphate buffered saline at the skin’s surface. The LA biosensor was fabricated by modifying screen printed carbon electrodes with osmium wired horseradish peroxidase and lactate oxidase. For continuous monitoring purposes, the microfluidic LA biosensor was connected with a flow cell made of polydimethylsiloxane (PDMS). The sampling device was fabricated by laminating a medical plaster on a PDMS flow-channel. Before the clinical tests, the characteristics of the LA biosensor were evaluated. Sufficient sensitivity (0.4232 nA/µM) and selectivity to LA (> 50fold against glucose, glutamic acid, phenylalanine, ammonia and ethanol) were confirmed. Besides, the medical plaster-based attachment contributed to the reduced leakage between the skin and the sampling device. Under the approval of the ethical committees, LA monitoring of patients with cardiovascular disease at the intensive care unit was carried out. As a result, temporal changes of sweat LA were successfully monitored.

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