Screen-Printed Lactate Biosensor Based on a Lox-Immobilized MgO-Templated Carbon for Continuously Monitoring of Lactate Concentration in Sweat

The aim of this study was to analyze the influence of two velocities of execution relative to blood lactate concentration in strength training exercise until the momentary concentric failure. Fifteen men (29.1 ± 5.9 years), trained, participated in the experiment. The volunteers performed three bench press sessions, with an interval of 48 hours between them. At the first session, individuals determined loads through the 10-12 RMs test. In the following two sessions, three series with 90 seconds of interval were performed, in the second session slow execution speed (cadence 3030) and later in the third session fast speed (cadence 1010). For statistical analysis, the Student-T test was used for an independent sample study and considered the value of probability (p) ≤ 0.05 statistically significant. By comparing the number of repetitions and time under tension of the two runs, all series compared to the first presented significant reductions (p < 0.05). The total work volume was higher with the fast speed (p < 0.05). The study revealed that rapid velocities (cadence 1010) present a higher concentration of blood lactate when compared to slow runs (cadence 3030). The blood lactate concentration, in maximum repetitions, is affected by the speed of execution.Keywords: resistance training, cadence, blood lactate.

Download Full-text

Caffeine supplementation for 4-day, followed by acute ingestion, did not impact triathlete output power after submaximal intensity exercise

Revista Brasileira de Fisiologia do Exercício ◽

10.33233/rbfe.v18i3.3230 ◽

2019 ◽

Vol 18 (3) ◽

pp. 118

Author(s):

Anderson Pontes Morales ◽

Felipe Sampaio-Jorge ◽

Thiago Barth ◽

Alessandra Alegre De Matos ◽

Luiz Felipe Da Cruz Rangel ◽

...

Keyword(s):

Blood Lactate ◽

Output Power ◽

Body Mass ◽

Effect Size ◽

Power Output ◽

Anaerobic Power ◽

Lactate Concentration ◽

Wingate Test ◽

Large Effect Size ◽

Acute Ingestion

Introduction: The aim of this study was to test the hypothesis that caffeine supplementation (6 mg·kg-1 body mass) for 4-days, followed by acute intake, would impact five male triathletes output power after performed submaximal intensity exercise. Methods: This was a randomized, double-blind, placebo-controlled crossover study, placebo (4-day) - placebo (acute) PP, placebo (4-days) -caffeine (acute) PC, and caffeine (4-day) - caffeine (acute) CC. Participants abstained from dietary caffeine sources for 4 days and ingested capsules containing either placebo or caffeine (6 mg.kg-1 body mass day in one absorption). The acute trials the capsules containing placebo or caffeine (6 mg.kg-1 body mass day in one absorption) were ingested 60min before completing exercise in a treadmill for 40min (80% VO2max) and to perform the Wingate test. Results: Blood lactate was determined before, 60min after ingestion, and immediately after the exercise on the treadmill, the Wingate test, and after the recovery (10-min). CC and PC trials did not change the cardiopulmonary variables (P>0.05) and the anaerobic power variables (peak/mean power output and fatigue index) (P>0.05). The PC trial compared with PP promoted improvements in the curve power output in 2 sec by 31.19% (large effect-size d = 1.08; P<0.05) and 3 sec by 20% (large effect-size d = 1.19; P<0.05). A 10min recovery was not sufficient to reduce blood lactate concentration in the PC trial compared with PP (PC, 13.73±2.66 vs. PP, 10.26±1.60 mmol.L-1; P<0.05, respectively) (P<0.05). Conclusion: In conclusion, these results indicate that caffeine supplementation (6 mg·kg-1 body mass) for 4 days, followed by acute ingestion, did not impact the triathletes output power after performed submaximal intensity exercise. Nutritional interventions may help researchers and athletes to adapt strategies for manipulating caffeine use.Key-words: caffeine metabolism, Wingate test, blood lactate, performance.

Download Full-text

RELATIONSHIPS BETWEEN RECALL OF PERCEIVED EXERTION AND BLOOD LACTATE CONCENTRATION IN A JUDO COMPETITION

Perceptual and Motor Skills ◽

10.2466/pms.92.3.1139-1148 ◽

2001 ◽

Vol 92 (3) ◽

pp. 1139 ◽

Cited By ~ 7

Author(s):

MIGUEL ANGEL SERRANO

Keyword(s):

Blood Lactate ◽

Perceived Exertion ◽

Lactate Concentration ◽

Blood Lactate Concentration

Download Full-text

Comparison of Two Tests to Determine the Maximal Aerobic Speed

Acta Facultatis Educationis Physicae Universitatis Comenianae ◽

10.2478/afepuc-2020-0021 ◽

2020 ◽

Vol 60 (2) ◽

pp. 252-262

Author(s):

Benhammou Saddek ◽

Jérémy B.J. Coquart ◽

Laurent Mourot ◽

Belkadi Adel ◽

Mokkedes Moulay Idriss ◽

...

Keyword(s):

Physiological Responses ◽

Intraclass Correlation ◽

Lactate Concentration ◽

Blood Lactate Concentration ◽

Random Order ◽

Standard Test ◽

Maximal Heart Rate ◽

Distance Runners ◽

Gold Standard Test ◽

Maximal Aerobic Speed

SummaryThe aims of this study were (a): to compare maximal physiological responses (maximal heart rate: HRmax and blood lactate concentration: [La-]) and maximal aerobic speed (MAS) achieved during a gold standard test (T-VAM) to those during a new test entitled: the 150-50 Intermittent Test (150-50IT), and (b): to test the reliability of the 150-50IT. Eighteen middle-distance runners performed, in a random order, the T-VAM and the 150-50IT. Moreover, the runners performed a second 150-50IT (retest). The results of this study showed that the MAS obtained during 150-50IT were significantly higher than the MAS during the T-VAM (19.1 ± 0.9 vs. 17.9 ± 0.9 km.h−1, p < 0.001). There was also significant higher values in HRmax (193 ± 4 vs. 191 ± 2 bpm, p = 0.011), [La-] (11.4 ± 0.4 vs. 11.0 ± 0.5 mmol.L−1, p = 0.039) during the 150-50IT. Nevertheless, significant correlations were noted for MAS (r = 0.71, p = 0.001) and HRmax (r = 0.63, p = 0.007). MAS obtained during the first 150-50IT and the retest were not significantly different (p = 0.76) and were significantly correlated (r = 0.94, p < 0.001, intraclass correlation coefficient = 0.93 and coefficient of variation = 6.8 %). In conclusion, the 150-50IT is highly reproducible, but the maximal physiological responses derived from both tests cannot be interchangeable in the design of training programs.

Download Full-text

Evaluation of pleural fluid lactate concentration for management transudate and exudate in pleural effusion

10.31923/pooltext-preprint-0705-6435-0048 ◽

2019 ◽

Author(s):

Rıdvan Cevlik ◽

Engin Ozakin ◽

Huseyin Yildirim ◽

Esref Genc ◽

Nurdan Acar ◽

...

Keyword(s):

Pleural Effusion ◽

Pleural Fluid ◽

Lactate Concentration

Download Full-text

Voltammetric Sensing of Uremic Toxin Indoxyl Sulfate Using High Performance Disposable Screen-Printed Graphene Electrode

Current Pharmaceutical Analysis ◽

10.2174/1573412911666150520000605 ◽

2015 ◽

Vol 12 (1) ◽

pp. 36-42 ◽

Cited By ~ 1

Author(s):

Hayati Filik ◽

Asiye A. Avan ◽

Sevda Aydar

Keyword(s):

High Performance ◽

Indoxyl Sulfate ◽

Uremic Toxin ◽

Graphene Electrode ◽

Voltammetric Sensing ◽

Screen Printed

Download Full-text

Facile and Fast Detection of Genistein in Derris scandens by Square Wave Voltammetry using a Cobalt(II) Phthalocyanine-Modified Screen-Printed Electrochemical Sensor

Current Analytical Chemistry ◽

10.2174/1573411014666180521091053 ◽

2020 ◽

Vol 16 (3) ◽

pp. 341-348

Author(s):

Surinya Traipop ◽

Suchada Chuanuwatanakul ◽

Orawon Chailapakul ◽

Eakkasit Punrat

Keyword(s):

Electrochemical Sensor ◽

Square Wave Voltammetry ◽

Silver Chloride ◽

Reference Electrode ◽

High Sensitivity ◽

Plastic Substrate ◽

Fast Analysis ◽

Square Wave ◽

Wave Voltammetry ◽

Screen Printed

Background: Recently, Derris scandens, a Thai herbal medicine with anti-inflammatory activity, is widely used as beverage and supplementary food. When the traditional medicine is a choice for health therapy, the simple and reliable equipment is required to control the suitable consuming amount of the active component. Objective: To develop the electrochemical sensor for genistein determination in Derris scandens with high sensitivity and rapid operation. Methods: An in-house screen-printed electrochemical sensor consisting of a three-electrode system was developed for genistein determination. A silver/silver chloride (Ag/AgCl) reference electrode, a carbon counter electrode and a carbon working electrode were prepared on a 0.3-mm-thick plastic substrate by the screen-printing technique using conductive ink. The dimensions of each sensor were 2.5×1.0 cm. Only 50 µL of sample solution was required on this device for the determination of genistein concentration by rapid response square wave voltammetry. Results: The oxidation peak of genistein appeared with good response in acidic media at a peak potential of 0.6 V. Moreover, the signal was enhanced by modifying the conductive carbon ink with cobalt( II) phthalocyanine. Under the optimized conditions, the linear range was found to be 2.5-150 µM and the detection limit was 1.5 µM. Moreover, the small volume extraction was successfully developed without any further pre-concentration. This proposed method was applied to determine genistein in Derris scandens with satisfying results. Conclusion: The proposed method is promising as an alternative method for genistein determination with facile and fast analysis.

Download Full-text