An F-box E3 ubiquitin ligase-coding gene AtDIF1 is involved in Arabidopsis salt and drought stress responses in an abscisic acid-dependent manner

2017 ◽  
Vol 138 ◽  
pp. 21-35 ◽  
Author(s):  
Shuai Gao ◽  
Jian Bo Song ◽  
Ye Wang ◽  
Zhi Min Yang
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Ubiquitin Ligase ◽  
Stress Responses ◽  
E3 Ubiquitin Ligase ◽  
Dependent Manner ◽  
Salt And Drought Stress

scholarly journals The Arabidopsis RING E3 Ubiquitin Ligase AtAIRP2 Plays Combinatory Roles with AtAIRP1 in Abscisic Acid-Mediated Drought Stress Responses

2011 ◽  
Vol 157 (4) ◽  
pp. 2240-2257 ◽  
Author(s):  
Seok Keun Cho ◽  
Moon Young Ryu ◽  
Dong Hye Seo ◽  
Bin Goo Kang ◽  
Woo Taek Kim
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Ubiquitin Ligase ◽  
Stress Responses ◽  
E3 Ubiquitin Ligase ◽  
Ring E3

scholarly journals miR394 and LCR are involved in Arabidopsis salt and drought stress responses in an abscisic acid-dependent manner

2013 ◽  
Vol 13 (1) ◽  
pp. 210 ◽  
Author(s):  
Jian Song ◽  
Shuai Gao ◽  
Di Sun ◽  
Hua Li ◽  
Xia Shu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Stress Responses ◽  
Dependent Manner ◽  
Salt And Drought Stress

Identification of Ubiquitin Ligase From Grapevine Ring C3H2C3E3 and Characterization of Drought Resistance Function of VyRCHC114

2020 ◽  
Author(s):  
Yihe Yu ◽  
Shengdi Yang ◽  
Lu Bian ◽  
Keke Yu ◽  
Xiangxuan Meng ◽  
...  
Keyword(s):  
Drought Stress ◽  
Ubiquitin Ligase ◽  
Stress Responses ◽  
Phylogenetic Trees ◽  
Expression Profiles ◽  
E3 Ubiquitin Ligase ◽  
Pcr Analysis ◽  
Biotic And Abiotic Stress ◽  
Element Analysis

Abstract Background: RING is one of the largest E3 ubiquitin ligase families and C3H2C3 type is the largest subfamily of RING, playing an important role in plants’ development and growth and their biotic and abiotic stress responses. Results: A total of 143 RING C3H2C3-type genes (RCHCs) were discovered from the grapevine genome and separated into groups (I-XI) according to their phylogenetic analysis, with these genes named according to their positions on chromosomes. Gene replication analysis showed that tandem duplications play a predominant role in the expansion of VyRCHCs family together. Structural analysis showed that most VyRCHCs(67.13%) had no more than 2 introns, while genes clustered together based on phylogenetic trees had similar motifs and evolutionarily conserved structures. Cis-acting element analysis showed the diversity of VyRCHCs regulation. The expression profiles of eight DEGs in RNA-Seq after drought stress were similar to those in qRT-PCR analysis. The in vitro ubiquitin experiment showed that VyRCHC114 had E3 ubiquitin ligase activity, overexpression of VyRCHC114 in Arabidopsis improved drought tolerance, moreover, the transgenic plant survival rate increased by 30%, accompanied by changing of electrolyte leakage, chlorophyll content and the activities of SOD, POD, APX and CAT were changed. AtCOR15a, AtRD29A, AtERD15 and AtP5CS1 were expressed quantitatively, the results showed that they participated in the drought stress response may be regulated by the expression of VyRCHC114.Conclusions: Valuable new information on the evolution of grapevine RCHCs and its relevance for studying the functional characteristics of grapevine VyRCHC114 genes under drought stress emerged from this research.

ZmRFP1, the putative ortholog of SDIR1, encodes a RING-H2 E3 ubiquitin ligase and responds to drought stress in an ABA-dependent manner in maize

Gene ◽  
2012 ◽  
Vol 495 (2) ◽  
pp. 146-153 ◽  
Author(s):  
Zongliang Xia ◽  
Quanjun Liu ◽  
Jianyu Wu ◽  
Junqiang Ding
Keyword(s):  
Drought Stress ◽  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
Dependent Manner

scholarly journals Regulation of p53 and MDM2 Activity by MTBP

2005 ◽  
Vol 25 (2) ◽  
pp. 545-553 ◽  
Author(s):  
Mark Brady ◽  
Nikolina Vlatković ◽  
Mark T. Boyd
Keyword(s):  
Ubiquitin Ligase ◽  
Stress Responses ◽  
Nuclear Export ◽  
Cellular Response ◽  
E3 Ubiquitin Ligase ◽  
Ring Finger ◽  
Dependent Manner ◽  
Γ Irradiation ◽  
Ubiquitin Ligase Activity ◽  
Wide Range

ABSTRACT p53 is a critical coordinator of a wide range of stress responses. To facilitate a rapid response to stress, p53 is produced constitutively but is negatively regulated by MDM2. MDM2 can inhibit p53 in multiple independent ways: by binding to its transcription activation domain, inhibiting p53 acetylation, promoting nuclear export, and probably most importantly by promoting proteasomal degradation of p53. The latter is achieved via MDM2's E3 ubiquitin ligase activity harbored within the MDM2 RING finger domain. We have discovered that MTBP promotes MDM2-mediated ubiquitination and degradation of p53 and also MDM2 stabilization in an MDM2 RING finger-dependent manner. Moreover, using small interfering RNA to down-regulate endogenous MTBP in unstressed cells, we have found that MTBP significantly contributes to MDM2-mediated regulation of p53 levels and activity. However, following exposure of cells to UV, but not γ-irradiation, MTBP is destabilized as part of the coordinated cellular response. Our findings suggest that MTBP differentially regulates the E3 ubiquitin ligase activity of MDM2 towards two of its most critical targets (itself and p53) and in doing so significantly contributes to MDM2-dependent p53 homeostasis in unstressed cells.

The F-box E3 ubiquitin ligase BAF1 mediates the degradation of the brassinosteroid-activated transcription factor BES1 through selective autophagy in Arabidopsis

2021 ◽  
Author(s):  
Ping Wang ◽  
Trevor M Nolan ◽  
Natalie M Clark ◽  
Hao Jiang ◽  
Christian Montes-Serey ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Ubiquitin Ligase ◽  
Stress Responses ◽  
E3 Ubiquitin Ligase ◽  
Dependent Manner ◽  
Selective Autophagy ◽  
Sucrose Starvation ◽  
Autophagic Degradation ◽  
Autophagy Pathway

Abstract Brassinosteroids (BRs) regulate plant growth, development, and stress responses by activating the core transcription factor BRI1-EMS-SUPPRESSOR1 (BES1), whose degradation occurs through the proteasome and autophagy pathways. The E3 ubiquitin ligase(s) that modify BES1 for autophagy-mediated degradation remain to be fully defined. Here, we identified an F-box family E3 ubiquitin ligase named BES1-ASSOCIATED F-BOX1 (BAF1) in Arabidopsis thaliana. BAF1 interacts with BES1 and mediates its ubiquitination and degradation. Our genetic data demonstrated that BAF1 inhibits BR signaling in a BES1-dependent manner. Moreover, BAF1 targets BES1 for autophagic degradation in a selective manner. BAF1-triggered selective autophagy of BES1 depends on the ubiquitin binding receptor DOMINANT SUPPRESSOR OF KAR2 (DSK2). Sucrose starvation-induced selective autophagy of BES1, but not bulk autophagy, was significantly compromised in baf1 mutant and BAF1-ΔF (BAF1 F-box decoy) overexpression plants, but clearly increased by BAF1 overexpression. The baf1 and BAF1-ΔF overexpression plants had increased BR-regulated growth but were sensitive to long-term sucrose starvation, while BAF1 overexpression plants had decreased BR-regulated growth but were highly tolerant of sucrose starvation. Our results not only established BAF1 as an E3 ubiquitin ligase that targets BES1 for degradation through selective autophagy pathway, but also revealed a mechanism for plants to reduce growth during sucrose starvation.

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