scholarly journals Seminal plasma metabolome in relation to semen quality and urinary phthalate metabolites among Chinese adult men

2019 ◽  
Vol 129 ◽  
pp. 354-363 ◽  
Author(s):  
Yi-Xin Wang ◽  
Yan Wu ◽  
Heng-Gui Chen ◽  
Peng Duan ◽  
Liang Wang ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Phthalate Metabolites ◽  
Adult Men ◽  
Chinese Adult ◽  
Plasma Metabolome

Associations of urinary 5-methyl-2′-deoxycytidine and 5-hydroxymethyl-2′-deoxycytidine with phthalate exposure and semen quality in 562 Chinese adult men

2016 ◽  
Vol 94 ◽  
pp. 583-590 ◽  
Author(s):  
Yitao Pan ◽  
Jun Jing ◽  
Leo W.Y. Yeung ◽  
Nan Sheng ◽  
Hongxia Zhang ◽  
...  
Keyword(s):  
Semen Quality ◽  
Adult Men ◽  
Chinese Adult ◽  
Phthalate Exposure

scholarly journals Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Maja Zakošek Pipan ◽  
Petra Zrimšek ◽  
Breda Jakovac Strajn ◽  
Katarina Pavšič Vrtač ◽  
Tanja Knific ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Quality Characteristics ◽  
Freezing And Thawing ◽  
Additional Tool ◽  
Bull Semen ◽  
Bull Sperm ◽  
Fresh Semen

ABSTRACT Background Wide variation in fertility rates is observed when using frozen bull semen, even when the bulls have met quality standards for semen production. Therefore, a simple and reliable test to assess the freezing potential of bull semen based on the analysis of fresh semen or blood would be of great value. Attention is now turning to assessment of seminal plasma components such as proteins and elements. In the present study, the concentrations of macro- and microelements in fresh bull semen plasma and in serum and their correlation with quality characteristics of fresh semen and with semen quality after freezing and thawing were determined. Ejaculates were collected from 30 mature bulls, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability and DNA fragmentation were determined on the day of collection and after freezing and thawing. The concentrations of macroelements (Na, Mg, K and Ca) and microelements (Cu, Fe, Zn and Se) were determined in the seminal plasma and serum. The semen samples were classified into satisfactory and unsatisfactory groups according to the fresh semen quality. Results Zinc and Se levels measured in serum were associated with almost all fresh and frozen-thawed semen quality characteristics, while Fe levels were associated only with acrosomal defects in fresh semen. Zinc and Fe levels in fresh seminal plasma were associated with various quality characteristics of fresh and frozen-thawed semen, while Se level in fresh seminal plasma was not associated with any of the semen quality characteristics. Conclusions Microelements were shown to be useful as biomarkers involved in the analysis of bull sperm quality and could be used as an additional tool to predict bull semen quality after freezing and thawing. Our results confirm that the analysis of Zn and Se levels in serum and Zn, Cu and Fe levels in fresh seminal plasma can provide information to discriminate between bull semen samples with spermatozoa with high or low cryotolerance.

scholarly journals Semen quality, lipid peroxidation, and seminal plasma antioxidant status in horses with different intensities of physical exercise

2013 ◽  
Vol 82 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Helena Härtlová ◽  
Radko Rajmon ◽  
Iva Krontorádová ◽  
Jiří Mamica ◽  
Lukáš Zita ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Seminal Plasma ◽  
Aspartate Aminotransferase ◽  
Semen Quality ◽  
Antioxidant Activities ◽  
Membrane Damage ◽  
Antioxidant Enzyme Activities ◽  
Reference Ranges ◽  
Stress Symptoms ◽  
Plasma Antioxidant

The aim of this study was to compare markers of semen quality, sperm membrane damage, and the seminal plasma antioxidant activity in warmblood stallions with and without sport workload stress. Four stallions were used for breeding only (control) and four both for breeding and competition in jumping. Semen samples were collected at 14-day intervals (from June to August) from each stallion (5 ejaculates per stallion). Immediately after sperm collection, a conventional examination of the ejaculate was processed. Catalytic activities of enzymes aspartate aminotransferase, alanin aminotransferase, glutathione peroxidase, superoxide dismutase and indicator of lipoperoxidation - F2α isoprostanes were measured in samples of seminal plasma. Contrary to basic semen quality indicators, the values of seminal plasma pH, aspartate aminotransferase and alanin aminotransferase were significantly (P < 0.05) impaired in the physically stressed stallions. Also, the level of F2α isoprostanes and the activity of superoxide dismutase were significantly (P < 0.05) increased by stress. The antioxidant activities of superoxide dismutase and glutathion peroxidase increased during the monitored period and reflected changes in F2α isoprostane concentration. We can conclude that even the conventional basic sperm indicators stay within the reference ranges of the biochemical indicators of seminal plasma such as pH or AST/ALT activity may be negatively influenced by sport workload stress. Increased concentrations of F2α isoprostanes indicate that lipoperoxidation can be a mechanism of cell membrane destabilization, which is counteracted by an increase of antioxidant enzyme activities. This is the first report of oxidative stress symptoms in normospermic equine semen in relation to stallion sport workload.

Neonicotinoid insecticide metabolites in the seminal plasma: Associations with semen quality

2021 ◽  
pp. 151407
Author(s):  
Aizhen Wang ◽  
Yanjian Wan ◽  
Lixiao Zhou ◽  
Wei Xia ◽  
Yinsheng Guo ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Neonicotinoid Insecticide

Seasonal variations in hormones and enzymes of seminal plasma and its relationship with semen quality in crossbred cattle bulls

2018 ◽  
Vol 51 (4) ◽  
pp. 633-643
Author(s):  
Nitika S. Jalmeria ◽  
Sunil Panth ◽  
Sujata Pandita ◽  
A.K. Roy ◽  
Manju Ashutosh ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Seasonal Variations ◽  
Semen Quality ◽  
Crossbred Cattle

scholarly journals Spectrophotometric Analysis of the Resazurin Reduction Test as a Tool for Assessing Canine Semen Quality

2013 ◽  
Vol 57 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Rafał Strzeżek ◽  
Krystyna Filipowicz ◽  
Marta Stańczak ◽  
Władysław Kordan
Keyword(s):  
Sperm Motility ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Spectrophotometric Analysis ◽  
Normal Morphology ◽  
Additional Tool ◽  
Reduction Test ◽  
Highly Correlated

Abstract The resazurin reduction test (RRT) was subjected to spectrophotometric analysis to evaluate the quality of canine semen. Twenty four samples of canine semen were analysed. The absorption peaks for resazurin and resorufin were determined at 615 and 580 nm, respectively. The RRT ratio (RRTsperm-the ratio for samples containing spermatozoa, RRTplasma-the ratio for samples containing seminal plasma) was calculated by dividing the absorbance at 580 nm by the absorbance at 615 nm. Spearman’s correlation test was used to determine the significance of correlations between the analysed sperm parameters and the results of the resazurin reduction assay. The RRT ratio was highly correlated with sperm motility (r=0.68, P<0.01), progressive sperm motility (r=0.61, P<0.01), the subpopulation of cells with rapid velocity (r=0.72, P<0.01), and the subpopulation of cells with medium velocity (r= -0.54, P<0.05). A negative correlation was observed between the reducing capacity of seminal plasma vs. sperm with plasma membrane integrity (r= -0.60, P<0.01) and sperm with normal morphology (r= -0.58, P<0.01). The RRT test can be used as an additional tool for evaluation of the quality of canine semen.

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