Concentrations of vanadium in urine and seminal plasma in relation to semen quality parameters, spermatozoa DNA damage and serum hormone levels

This study is designed to evaluate the effect of using parsley seeds (PAS) at different levels as a natural on semen quality and reduce the effect of lipidperoxidation of bucks. Forty New Zealand rabbit bucks 8 month old were divided into four groups (each 10 rabbit bucks). The groups were divided as follows: Control diet (free from PAS), T1: Control diet + 5g/kg PAS, T2: Control diet + 10g/kg PAS; T3: Control diet + 15g/kg PAS. Semen sample were collected twice a week; from each rabbit buck by artificial vagina. The first semen collection was used to evaluate semen quality parameters. However, the second semen collection was used for analysis semen plasma. Results revealed that feeding diets containing different levels of PAS significantly (P<0.05) increased semen volume, mass activity, individual motility, PSV, sperm concentration, total sperm and seminal plasma activity of ALP enzyme and significant (P<0.05) decrease in dead sperm and seminal plasma concentrations of total lipids, triglycerides, cholesterol and MDA and seminal plasma activities of AST and ALT enzymes and TAC as compared with the control group. In conclusion, dietary supplementation with different levels of PAS, especially at the level of 15 g/kg (T3) resulted in significant improvement in semen quality, antioxidant status and reproductive traits of rabbit bucks.

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Transferrin Concentration in Seminal Plasma with Special Reference to Serum Hormone Levels in Infertile Men

Archives of Andrology ◽

10.3109/01485019308988363 ◽

1993 ◽

Vol 30 (1) ◽

pp. 13-21 ◽

Cited By ~ 4

Author(s):

C. Irisawa ◽

T. Nakada ◽

Y. Kubota ◽

I. Sasagawa ◽

Y. Adachi ◽

...

Keyword(s):

Special Reference ◽

Seminal Plasma ◽

Hormone Levels ◽

Infertile Men ◽

Serum Hormone

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Are semen quality parameters sufficient for biomonitoring spermatozoa DNA integrity and oxidatively damaged DNA

Biomonitoring ◽

10.1515/bimo-2015-0004 ◽

2015 ◽

Vol 2 (1) ◽

Author(s):

Hueiwang Anna Jeng ◽

Ruei-Nian Li ◽

Wen-Yi Lin

Keyword(s):

Dna Damage ◽

Dna Fragmentation ◽

Semen Quality ◽

Quality Parameters ◽

Semen Parameters ◽

Sperm Chromatin ◽

Dna Integrity ◽

Phase System ◽

Sperm Dna Fragmentation ◽

Sperm Dna

Abstract:The present study aimed to investigate the relationship between semen quality parameters and DNA integrity, and determine whether semen quality parameters could serve as a reliable biomarker for monitoring sperm DNA damage. Conventional semen parameters from a total of 202 male human subjects were analyzed. DNA fragmentation and 8-oxo-7,8-dihydro-2′- deoxyguanosine (8-oxoGuo) were used to assess sperm DNA integrity. DNA fragmentation was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and sperm chromatin structure assay (SCSA), while 8-oxodGuo was quantified by the liquid chromatography/tandem mass spectrometry (LC-MS/MS) coupled with an on-line solid phase system. The levels of 8-oxodGuo levels in sperm were related to the percentages of DNA fragmentation measured by both the TUNEL and SCSA (r = 0.22, p = 0.048; r = 0.12, p = 0.039). Sperm vitality, motility and morphology from all of the participants exhibited a weak correlation with the levels of 8-oxodGuo and the percentages of DNA fragmentation. Semen quality parameters may be independent of the formation of DNA fragmentation and oxidative adducts in sperm. Semen quality parameters may be insufficient to monitor sperm DNA fragmentation and oxidative damage. DNA damage in sperm is recommended to be included in routine measurements.

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Phthalate exposure in association with serum hormone levels, sperm DNA damage and spermatozoa apoptosis: A cross-sectional study in China

Environmental Research ◽

10.1016/j.envres.2015.11.023 ◽

2016 ◽

Vol 150 ◽

pp. 557-565 ◽

Cited By ~ 43

Author(s):

Yi-Xin Wang ◽

Qiang Zeng ◽

Yang Sun ◽

Ling You ◽

Peng Wang ◽

...

Keyword(s):

Dna Damage ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Hormone Levels ◽

Sperm Dna Damage ◽

Phthalate Exposure ◽

Sperm Dna ◽

Serum Hormone

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Human Semen Quality, Sperm DNA Damage, and the Level of Urinary Concentrations of 1N and TCPY, the Biomarkers of Nonpersistent Insecticides

American Journal of Men s Health ◽

10.1177/1557988318816598 ◽

2018 ◽

Vol 13 (1) ◽

pp. 155798831881659 ◽

Cited By ~ 3

Author(s):

Emila Dziewirska ◽

Michał Radwan ◽

Bartosz Wielgomas ◽

Anna Klimowska ◽

Paweł Radwan ◽

...

Keyword(s):

Dna Damage ◽

Environmental Exposure ◽

Semen Quality ◽

Semen Analysis ◽

Positive Association ◽

Quality Parameters ◽

Normal Morphology ◽

Sperm Dna Damage ◽

Fragmentation Index ◽

Sperm Dna

The aim of the study was to evaluate the association between environmental exposure to nonpersistent insecticides and semen quality (concentration, motility, morphology, computer-aided semen analysis [CASA] parameters, and sperm DNA damage). Urine samples ( n = 315) collected from men who attended the infertility clinic with normal semen concentration of 15 to 300 mln/ml and age under 45 years were analyzed for two metabolites (1-naphthol [1N] and 3,5,6-trichloro-2-pyridinol [TCPY]) of nonpersistent insecticides. Participants provided semen, blood, and saliva samples; additionally, men filled a detailed questionnaire. The results identified that urinary TCPY concentration was significantly associated with a decrease in motility; also there was a positive association between TCPY and DNA fragmentation index (DFI). 1N concentration was negatively associated with a percentage of sperm with normal morphology and positively with one of the CASA parameters (curvilinear velocity [VCL]). The results suggest that environmental exposure to nonpersistent insecticides may have an impact on semen quality parameters and sperm DNA damage.

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