Synergistic enzyme cocktail between levansucrase and inulosucrase for superb levan-type fructooligosaccharide synthesis

Author(s):  
Karan Wangpaiboon ◽  
Methus Klaewkla ◽  
Thanapon Charoenwongpaiboon ◽  
Napas Vongkusolkit ◽  
Pawinee Panpetch ◽  
...  
Keyword(s):  
AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Arashdeep Kaur ◽  
Sanjeev Kumar Soni ◽  
Shania Vij ◽  
Praveen Rishi

AbstractBiofilm formation on both biotic and abiotic surfaces accounts for a major factor in spread of antimicrobial resistance. Due to their ubiquitous nature, biofilms are of great concern for environment as well as human health. In the present study, an integrated process for the co-production of a cocktail of carbohydrases from a natural variant of Aspergillus niger was designed. The enzyme cocktail was found to have a noteworthy potential to eradicate/disperse the biofilms of selected pathogens. For application of enzymes as an antibiofilm agent, the enzyme productivities were enhanced by statistical modelling using response surface methodology (RSM). The antibiofilm potential of the enzyme cocktail was studied in terms of (i) in vitro cell dispersal assay (ii) release of reducing sugars from the biofilm polysaccharides (iii) the effect of enzyme treatment on biofilm cells and architecture by confocal laser scanning microscopy (CLSM). Potential of the enzyme cocktail to disrupt/disperse the biofilm of selected pathogens from biopolymer surfaces was also assessed by field emission scanning electron microscopy (FESEM) analysis. Further, their usage in conjunction with antibiotics was assessed and it was inferred from the results that the use of enzyme cocktail augmented the efficacy of the antibiotics. The study thus provides promising insights into the prospect of using multiple carbohydrases for management of heterogeneous biofilms formed in natural and clinical settings.


2009 ◽  
Vol 83 (5) ◽  
pp. 839-848 ◽  
Author(s):  
V. Blättel ◽  
K. Wirth ◽  
H. Claus ◽  
B. Schlott ◽  
P. Pfeiffer ◽  
...  

ChemCatChem ◽  
2016 ◽  
Vol 8 (18) ◽  
pp. 2898-2902 ◽  
Author(s):  
Hanan M. A. Moustafa ◽  
Eui-Jin Kim ◽  
Zhiguang Zhu ◽  
Chang-Hao Wu ◽  
Taha I. Zaghloul ◽  
...  

2018 ◽  
Vol 2 (5) ◽  
pp. 1048-1056 ◽  
Author(s):  
Rui Zhai ◽  
Jinguang Hu ◽  
Jack N. Saddler

The key enzyme activities were selectively inhibited and/or deactivated by water-soluble components derived from pretreated lignocellulosic biomass.


2018 ◽  
Vol 14 (3) ◽  
pp. 1700744 ◽  
Author(s):  
Musashi Takenaka ◽  
Jae M. Lee ◽  
Prihardi Kahar ◽  
Chiaki Ogino ◽  
Akihiko Kondo

2018 ◽  
Vol 37 (2) ◽  
pp. 149-156 ◽  
Author(s):  
C. Marzo ◽  
A.B. Díaz ◽  
I. Caro ◽  
A. Blandino

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L-1 were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.


Author(s):  
Flávio Augusto Cardozo ◽  
William Marcondes Facchinatto ◽  
Luiz Alberto Colnago ◽  
Sergio Paulo Campana-Filho ◽  
Adalberto Pessoa

Sign in / Sign up

Export Citation Format

Share Document