Molecular and behavioral assessment in larval zebrafish (Danio rerio) following exposure to environmentally relevant levels of the antineoplastic cyclophosphamide

2022 ◽  
pp. 103809
Author(s):  
Emma Ivantsova ◽  
Michelle Huang ◽  
Andrew S. Wengrovitz ◽  
Christopher L. Souders ◽  
Christopher J. Martyniuk
Keyword(s):  
Danio Rerio ◽  
Behavioral Assessment ◽  
Larval Zebrafish

Anatomical and Behavioral Assessment of Larval Zebrafish (Danio rerio) Reared in Anacostia River Water Samples

2020 ◽  
Vol 78 (4) ◽  
pp. 525-535
Author(s):  
Rebecca L. Wilken ◽  
Aikerim Imanalieva ◽  
Stephen MacAvoy ◽  
Victoria P. Connaughton
Keyword(s):  
River Water ◽  
Danio Rerio ◽  
Water Samples ◽  
Behavioral Assessment ◽  
Anacostia River ◽  
Larval Zebrafish ◽  
River Water Samples

Loss of M2 muscarinic receptor function inhibits development of hypoxic bradycardia and alters cardiac β-adrenergic sensitivity in larval zebrafish (Danio rerio)

2009 ◽  
Vol 297 (2) ◽  
pp. R412-R420 ◽  
Author(s):  
Shelby L. Steele ◽  
Kwok Hong Andy Lo ◽  
Vincent Wai Tsun Li ◽  
Shuk Han Cheng ◽  
Marc Ekker ◽  
...  
Keyword(s):  
Heart Rate ◽  
Muscarinic Receptor ◽  
Danio Rerio ◽  
Antisense Oligonucleotides ◽  
Adrenergic Receptor ◽  
Larval Zebrafish ◽  
M2 Muscarinic Receptor ◽  
Β Adrenergic Receptors ◽  
Relative Mrna Expression

Fish exposed to hypoxia develop decreased heart rate, or bradycardia, the physiological significance of which remains unknown. The general muscarinic receptor antagonist atropine abolishes the development of this hypoxic bradycardia, suggesting the involvement of muscarinic receptors. In this study, we tested the hypothesis that the hypoxic bradycardia is mediated specifically by stimulation of the M2 muscarinic receptor, the most abundant subtype in the vertebrate heart. Zebrafish ( Danio rerio) were reared at two levels of hypoxia (30 and 40 Torr Po2) from the point of fertilization. In hypoxic fish, the heart rate was significantly lower than in normoxic controls from 2 to 10 days postfertilization (dpf). At the more severe level of hypoxia (30 Torr Po2), there were significant increases in the relative mRNA expression of M 2 and the cardiac type β-adrenergic receptors ( β1AR, β2aAR, and β2bAR) at 4 dpf. The hypoxic bradycardia was abolished (at 40 Torr Po2) or significantly attenuated (at 30 Torr Po2) in larvae experiencing M2 receptor knockdown (using morpholino antisense oligonucleotides). Sham-injected larvae exhibited typical hypoxic bradycardia in both hypoxic regimens. The expression of β1AR, β2aAR, β2bAR, and M 2 mRNA was altered at various stages between 1 and 4 dpf in larvae experiencing M2 receptor knockdown. Interestingly, M2 receptor knockdown revealed a cardioinhibitory role for the β2-adrenergic receptor. This is the first study to demonstrate a specific role of the M2 muscarinic receptor in the initiation of hypoxic bradycardia in fish.

On the effects of iron ore tailings micro/nanoparticles in embryonic and larval zebrafish (Danio rerio)

2021 ◽  
Vol 759 ◽  
pp. 143456
Author(s):  
Vítor Otacílio de Almeida ◽  
Talita Carneiro Brandão Pereira ◽  
Lilian de Souza Teodoro ◽  
Manuella Escobar ◽  
Carolina Junqueira Ordovás ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Iron Ore ◽  
Larval Zebrafish ◽  
Iron Ore Tailings

scholarly journals Fumonisin B1 Production by Fusarium Species and Mycotoxigenic Effect on Larval Zebrafish

2020 ◽  
Vol 31 (3) ◽  
pp. 91-107 ◽  
Author(s):  
Najihah Azman ◽  
Nur Ain Izzati Mohd Zainudin ◽  
Wan Norhamidah Wan Ibrahim
Keyword(s):  
Liquid Chromatography ◽  
Danio Rerio ◽  
Morphological Changes ◽  
Fusarium Species ◽  
Fumonisin B1 ◽  
Larval Zebrafish ◽  
Zebrafish Larvae ◽  
Significant Difference ◽  
Successful Amplification ◽  
Fast Liquid Chromatography

Fumonisin B1 (FB1) is a common mycotoxin produced by Fusarium species particularly F. proliferatum and F. verticillioides. The toxin produced can cause adverse effects on humans and animals. The objectives of this study were to detect the production of FB1 based on the amplification of FUM1 gene, to quantify FB1 produced by the isolates using Ultra-fast Liquid Chromatography (UFLC) analysis, to examine the embryotoxicity effect of FB1 and to determine EC50 toward the larvae of zebrafish (Danio rerio). Fifty isolates of Fusarium species were isolated from different hosts throughout Malaysia. Successful amplification of the FUM1 gene showed the presence of this gene (800 bp) in the genome of 48 out of 50 isolates. The highest level of FB1 produced by F. proliferatum isolate B2433 was 6677.32 ppm meanwhile F. verticillioides isolate J1363 was 954.01 ppm. From the assessment of embryotoxicity test of FB1 on larvae of zebrafish, five concentrations of FB1 (0.43 ppm, 0.58 ppm, 0.72 ppm, 0.87 ppm and 1.00 ppm) were tested. Morphological changes of the FB1 exposed-larvae were observed at 24 to 168 hpf. The mortality rate and abnormality of zebrafish larvae were significantly increased at 144 hpf exposure. Meanwhile, the spontaneous tail coiling showed a significant difference. There were no significant differences in the heartbeat rate. As a conclusion, the presence of FUM1 in every isolate can be detected by FUM1 gene analysis and both of the species produced different concentrations of FB1. This is the first report of FB1 produced by Fusarium species gave a significant effect on zebrafish development.

Joint acute and endocrine disruptive toxicities of malathion, cypermethrin and prochloraz to embryo-larval zebrafish, Danio rerio

Chemosphere ◽  
2017 ◽  
Vol 166 ◽  
pp. 63-71 ◽  
Author(s):  
Dongmei Guo ◽  
Yanhua Wang ◽  
Yongzhong Qian ◽  
Chen Chen ◽  
Bining Jiao ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Larval Zebrafish

The pyrethroid esfenvalerate induces hypoactivity and decreases dopamine transporter expression in embryonic/larval zebrafish (Danio rerio)

Chemosphere ◽  
2020 ◽  
Vol 243 ◽  
pp. 125416 ◽  
Author(s):  
Xiao H. Wang ◽  
Christopher L. Souders ◽  
Priscilla Xavier ◽  
Xiao Y. Li ◽  
Bing Yan ◽  
...  
Keyword(s):  
Dopamine Transporter ◽  
Danio Rerio ◽  
Larval Zebrafish ◽  
Transporter Expression

Residual molecular and behavioral effects of the phenylpyrazole pesticide fipronil in larval zebrafish (Danio rerio) following a pulse embryonic exposure

2020 ◽  
Vol 36 ◽  
pp. 100743
Author(s):  
Ashley Eadie ◽  
Isabel Cristina Vasquez ◽  
Xuefang Liang ◽  
Xiaohong Wang ◽  
Christopher L. Souders ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Behavioral Effects ◽  
Larval Zebrafish ◽  
Embryonic Exposure
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