Effects of acute mGluR7 PAM on changes in short-time plasticity and mGluR7 levels after chronic treatment with mGluR5 antagonist and COX-2 inhibitor

Tumor microenvironment is composed of tumor cells, fibroblasts, and infiltrating immune cells, which all work together and create an inflammatory environment favoring tumor progression. The present study aimed to investigate the role of the desmoplastic stroma in pancreatic ductal adenocarcinoma (PDAC) regarding expression of inflammatory factors and infiltration of immune cells and their impact on the clinical outcome. The PDAC tissues examined expressed significantly increased levels of immunomodulatory and chemotactic factors (IL-6, TGFβ, IDO, COX-2, CCL2, and CCL20) and immune cell-specific markers corresponding to macrophages, myeloid, and plasmacytoid dendritic cells (DCs) as compared to controls. Furthermore, short-time survivors had the lowest levels of DC markers. Immunostainings indicated that the different immune cells and inflammatory factors are mainly localized to the desmoplastic stroma. Therapies modulating the inflammatory tumor microenvironment to promote the attraction of DCs and differentiation of monocytes into functional DCs might improve the survival of PDAC patients.

Download Full-text

Chronic co-administration of mGluR5 antagonist (MTEP) with COX-2 inhibitor (NS398) affects DSCAM expression in prefrontal cortex and hippocampus in C57Bl/6J mice

10.26226/morressier.5971be82d462b80290b52e0b ◽

2017 ◽

Author(s):

Katarzyna Stachowicz

Keyword(s):

Prefrontal Cortex ◽

Mglur5 Antagonist ◽

Cox 2

Download Full-text

Morphological Features of Rat Gastric Mucosa after Acute and Chronic Treatment with Amtolmetin Guacyl: Comparison with Non-Selective and COX-2-Selective NSAIDs

Digestion ◽

10.1159/000074726 ◽

2003 ◽

Vol 68 (2-3) ◽

pp. 124-132 ◽

Cited By ~ 3

Author(s):

Giuseppina Morini ◽

Elena Guaita ◽

Mirka Lazzaretti ◽

Daniela Grandi ◽

Gabriella Coruzzi

Keyword(s):

Gastric Mucosa ◽

Chronic Treatment ◽

Morphological Features ◽

Acute And Chronic Treatment ◽

Rat Gastric Mucosa ◽

Cox 2

Download Full-text

Chronic co-administration of mGluR5 antagonist (MTEP) with COX-2 inhibitor (NS398) affects DSCAM expression in prefrontal cortex and hippocampus in C57Bl/6J mice

European Neuropsychopharmacology ◽

10.1016/s0924-977x(17)31407-4 ◽

2017 ◽

Vol 27 ◽

pp. S771 ◽

Cited By ~ 1

Author(s):

K. Stachowicz ◽

M. Sowa-Kucma ◽

P. Pańczyszyn-Trzewik ◽

P. Misztak ◽

G. Nowak ◽

...

Keyword(s):

Prefrontal Cortex ◽

Mglur5 Antagonist ◽

Cox 2

Download Full-text

What the replication reformation wrought

Behavioral and Brain Sciences ◽

10.1017/s0140525x18000857 ◽

2018 ◽

Vol 41 ◽

Cited By ~ 2

Author(s):

Barbara A. Spellman ◽

Daniel Kahneman

Keyword(s):

Reform Movement ◽

Short Time

AbstractReplication failures were among the triggers of a reform movement which, in a very short time, has been enormously useful in raising standards and improving methods. As a result, the massive multilab multi-experiment replication projects have served their purpose and will die out. We describe other types of replications – both friendly and adversarial – that should continue to be beneficial.

Download Full-text

Periodic Variations in the Coronal Green Line Intensity and their Connection with the White-light Coronal Structures

International Astronomical Union Colloquium ◽

10.1017/s0252921100064484 ◽

2000 ◽

Vol 179 ◽

pp. 197-200

Author(s):

Milan Minarovjech ◽

Milan Rybanský ◽

Vojtech Rušin

Keyword(s):

Time Resolution ◽

White Light ◽

Short Time Scale ◽

High Time Resolution ◽

Green Line ◽

Periodic Intensity ◽

Coronal Green Line ◽

Significant Intensity ◽

Short Time ◽

Coronal Structures

AbstractWe present an analysis of short time-scale intensity variations in the coronal green line as obtained with high time resolution observations. The observed data can be divided into two groups. The first one shows periodic intensity variations with a period of 5 min. the second one does not show any significant intensity variations. We studied the relation between regions of coronal intensity oscillations and the shape of white-light coronal structures. We found that the coronal green-line oscillations occur mainly in regions where open white-light coronal structures are located.

Download Full-text

Destruction of Actin Filaments by Osmium Tetroxide

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079772 ◽

1977 ◽

Vol 35 ◽

pp. 466-467

Author(s):

P. Maupin-Szamier ◽

T. D. Pollard

Keyword(s):

Actin Filaments ◽

Time Course ◽

Osmium Tetroxide ◽

Rabbit Muscle ◽

Muscle Actin ◽

Sodium Phosphate Buffer ◽

Transmission Electron ◽

The Difference ◽

Rates Of Decay ◽

Short Time

We have studied the destruction of rabbit muscle actin filaments by osmium tetroxide (OSO4) to develop methods which will preserve the structure of actin filaments during preparation for transmission electron microscopy.Negatively stained F-actin, which appears as smooth, gently curved filaments in control samples (Fig. 1a), acquire an angular, distorted profile and break into progressively shorter pieces after exposure to OSO4 (Fig. 1b,c). We followed the time course of the reaction with viscometry since it is a simple, quantitative method to assess filament integrity. The difference in rates of decay in viscosity of polymerized actin solutions after the addition of four concentrations of OSO4 is illustrated in Fig. 2. Viscometry indicated that the rate of actin filament destruction is also dependent upon temperature, buffer type, buffer concentration, and pH, and requires the continued presence of OSO4. The conditions most favorable to filament preservation are fixation in a low concentration of OSO4 for a short time at 0°C in 100mM sodium phosphate buffer, pH 6.0.

Download Full-text